LOCUS       BBa_K543030             1105 bp    DNA     linear       22-Nov-2024
DEFINITION  [I714030][J23066][I714030] (Constitutive expression)
ACCESSION   BBa_K543030
VERSION     BBa_K543030.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     oriT            44..398
                     /label=BBa_I714030
     misc_feature    407..500
                     /label=BBa_J23008
     misc_feature    509..605
                     /label=BBa_J23009
     stem_loop       625..668
                     /label=stem_loop
     misc_feature    614..693
                     /label=BBa_B0010
     terminator      614..693
                     /label=BBa_B0010
     stem_loop       709..728
                     /label=T7 TE
     misc_feature    735..735
                     /label=stop
     polyA_site      729..742
                     /label=polya
     misc_feature    702..742
                     /label=BBa_B0012
     terminator      702..742
                     /label=BBa_B0012
     oriT            751..1105
                     /label=BBa_I714030
     misc_feature    44..1105
                     /label=BBa_I714051
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagcctctgg tgactttatc
       61 cgtaaataat ttaacccact ccacaaaaag gctcaacagg ttggtggttc tcaccaccaa
      121 aagcaccaca ccccacgcaa aaacaagttt ttgctgattt ttctttataa atagagtgtt
      181 atgaaaaatt agtttctctt actctcttta tgatatttaa aaaagcggtg tcggcgcggc
      241 tacaacaacg cgccgacacc gttttgtagg ggtggtactg actattttta taaaaaacat
      301 tattttatat taggggtgct gctagcggcg cggtgtgttt ttttatagga taccgctagg
      361 ggcgctgcta gcggtgcgtc cctgtttgca ttatgaatta ctagagaccc aaaagcaaga
      421 ggtgattcta gttggtggtt aatgaaaatt aacttactta ctagaaatat ctctaaaaag
      481 ccagattatt aatccggctt tactagagcg ggcgggcggg cgggcgggat ccataaaaaa
      541 aaaacccaaa agcaagaggt gattctagtt aaaaaaaaaa agcttcccgc ccgcccgccc
      601 gcccgtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      661 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      721 ggtgggcctt tctgcgttta tatactagag cctctggtga ctttatccgt aaataattta
      781 acccactcca caaaaaggct caacaggttg gtggttctca ccaccaaaag caccacaccc
      841 cacgcaaaaa caagtttttg ctgatttttc tttataaata gagtgttatg aaaaattagt
      901 ttctcttact ctctttatga tatttaaaaa agcggtgtcg gcgcggctac aacaacgcgc
      961 cgacaccgtt ttgtaggggt ggtactgact atttttataa aaaacattat tttatattag
     1021 gggtgctgct agcggcgcgg tgtgtttttt tataggatac cgctaggggc gctgctagcg
     1081 gtgcgtccct gtttgcatta tgaat
//