LOCUS BBa_K543032 3615 bp DNA linear 22-Dec-2024 DEFINITION assay system of killing by conjugation ACCESSION BBa_K543032 VERSION BBa_K543032.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 oriT 44..398 /label=BBa_I714030 misc_feature 407..500 /label=BBa_J23008 misc_feature 509..605 /label=BBa_J23009 stem_loop 625..668 /label=stem_loop misc_feature 614..693 /label=BBa_B0010 terminator 614..693 /label=BBa_B0010 stem_loop 709..728 /label=T7 TE misc_feature 735..735 /label=stop polyA_site 729..742 /label=polya misc_feature 702..742 /label=BBa_B0012 terminator 702..742 /label=BBa_B0012 oriT 751..1105 /label=BBa_I714030 misc_feature 44..1105 /label=BBa_I714051 oriT 1114..1484 /label=BBa_J01003 misc_feature 1497..1500 /label=conserved RBS 1493..1504 /label=BBa_B0034 CDS 1511..2230 /label=GFP protein CDS 1511..2230 /label=BBa_E0040 stem_loop 2250..2293 /label=stem_loop misc_feature 2239..2318 /label=BBa_B0010 terminator 2239..2318 /label=BBa_B0010 stem_loop 2334..2353 /label=T7 TE misc_feature 2360..2360 /label=stop polyA_site 2354..2367 /label=polya misc_feature 2327..2367 /label=BBa_B0012 terminator 2327..2367 /label=BBa_B0012 misc_feature 1493..2367 /label=BBa_I13504 oriT 2376..2746 /label=BBa_J01003 misc_feature 2759..2762 /label=conserved RBS 2755..2766 /label=BBa_B0034 CDS 2773..3447 /label=mrfp1 misc_feature 3454..3478 /label=Help:Barcodes CDS 2773..3453 /label=BBa_E1010 stem_loop 3498..3541 /label=stem_loop misc_feature 3487..3566 /label=BBa_B0010 terminator 3487..3566 /label=BBa_B0010 stem_loop 3582..3601 /label=T7 TE misc_feature 3608..3608 /label=stop polyA_site 3602..3615 /label=polya misc_feature 3575..3615 /label=BBa_B0012 terminator 3575..3615 /label=BBa_B0012 misc_feature 2755..3615 /label=BBa_I13507 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagcctctgg tgactttatc 61 cgtaaataat ttaacccact ccacaaaaag gctcaacagg ttggtggttc tcaccaccaa 121 aagcaccaca ccccacgcaa aaacaagttt ttgctgattt ttctttataa atagagtgtt 181 atgaaaaatt agtttctctt actctcttta tgatatttaa aaaagcggtg tcggcgcggc 241 tacaacaacg cgccgacacc gttttgtagg ggtggtactg actattttta taaaaaacat 301 tattttatat taggggtgct gctagcggcg cggtgtgttt ttttatagga taccgctagg 361 ggcgctgcta gcggtgcgtc cctgtttgca ttatgaatta ctagagaccc aaaagcaaga 421 ggtgattcta gttggtggtt aatgaaaatt aacttactta ctagaaatat ctctaaaaag 481 ccagattatt aatccggctt tactagagcg ggcgggcggg cgggcgggat ccataaaaaa 541 aaaacccaaa agcaagaggt gattctagtt aaaaaaaaaa agcttcccgc ccgcccgccc 601 gcccgtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 661 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 721 ggtgggcctt tctgcgttta tatactagag cctctggtga ctttatccgt aaataattta 781 acccactcca caaaaaggct caacaggttg gtggttctca ccaccaaaag caccacaccc 841 cacgcaaaaa caagtttttg ctgatttttc tttataaata gagtgttatg aaaaattagt 901 ttctcttact ctctttatga tatttaaaaa agcggtgtcg gcgcggctac aacaacgcgc 961 cgacaccgtt ttgtaggggt ggtactgact atttttataa aaaacattat tttatattag 1021 gggtgctgct agcggcgcgg tgtgtttttt tataggatac cgctaggggc gctgctagcg 1081 gtgcgtccct gtttgcatta tgaattacta gaggccgcct tttcctcaat cgctcttcgt 1141 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca 1201 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca 1261 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc 1321 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag 1381 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa 1441 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaatactag agaaagagga 1501 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga 1561 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 1621 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 1681 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca 1741 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac 1801 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga 1861 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct 1921 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca 1981 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca 2041 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 2101 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca 2161 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata 2221 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 2281 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2341 cttcgggtgg gcctttctgc gtttatatac tagaggccgc cttttcctca atcgctcttc 2401 gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt 2461 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag 2521 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc 2581 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa 2641 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg 2701 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaatact agagaaagag 2761 gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt 2821 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt 2881 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc 2941 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg 3001 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt 3061 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt 3121 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg 3181 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct 3241 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa 3301 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac 3361 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt 3421 gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta 3481 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 3541 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 3601 ctttctgcgt ttata //