##gff-version 3
##sequence-region BBa_K543032 1 3615
BBa_K543032	.	engineered_region	44	1105	.	+	0	ID=BBa_I714051;Name=BBa_I714051
BBa_K543032	.	oriT	44	398	.	+	0	ID=BBa_I714030;Name=BBa_I714030;Parent=BBa_I714051
BBa_K543032	.	terminator	702	742	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I714051
BBa_K543032	.	polyA_site	729	742	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543032	.	stem_loop	709	728	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543032	.	engineered_region	702	742	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K543032	.	stop_codon	735	735	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K543032	.	terminator	614	693	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I714051
BBa_K543032	.	stem_loop	625	668	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543032	.	engineered_region	614	693	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543032	.	oriT	751	1105	.	+	0	ID=BBa_I714030;Name=BBa_I714030;Parent=BBa_I714051
BBa_K543032	.	mature_transcript_region	407	500	.	+	0	ID=BBa_J23008;Name=BBa_J23008;Parent=BBa_I714051
BBa_K543032	.	mature_transcript_region	509	605	.	+	0	ID=BBa_J23009;Name=BBa_J23009;Parent=BBa_I714051
BBa_K543032	.	oriT	2376	2746	.	+	0	ID=BBa_J01003;Name=BBa_J01003
BBa_K543032	.	oriT	1114	1484	.	+	0	ID=BBa_J01003;Name=BBa_J01003
BBa_K543032	.	engineered_region	2755	3615	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K543032	.	CDS	2773	3453	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K543032	.	CDS	2773	3447	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K543032	.	engineered_tag	3454	3478	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K543032	.	terminator	3575	3615	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K543032	.	polyA_site	3602	3615	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543032	.	stem_loop	3582	3601	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543032	.	engineered_region	3575	3615	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K543032	.	stop_codon	3608	3608	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K543032	.	ribosome_entry_site	2755	2766	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K543032	.	conserved_region	2759	2762	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K543032	.	terminator	3487	3566	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K543032	.	stem_loop	3498	3541	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543032	.	engineered_region	3487	3566	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543032	.	engineered_region	1493	2367	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_K543032	.	ribosome_entry_site	1493	1504	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_K543032	.	conserved_region	1497	1500	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K543032	.	terminator	2327	2367	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_K543032	.	polyA_site	2354	2367	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543032	.	stem_loop	2334	2353	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543032	.	engineered_region	2327	2367	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K543032	.	stop_codon	2360	2360	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K543032	.	terminator	2239	2318	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_K543032	.	stem_loop	2250	2293	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543032	.	engineered_region	2239	2318	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543032	.	CDS	1511	2230	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_K543032	.	CDS	1511	2230	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K543032	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K543032
ttgacggctagctcagtcctaggtacagtgctagctactagagcctctggtgactttatccgtaaataatttaacccac
tccacaaaaaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgat
ttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgc
ggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattagggg
tgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatg
aattactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctct
aaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagc
aagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagcctctggtgactttatccgtaaataatttaacccactcca
caaaaaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgattttt
ctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggct
acaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgct
gctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaatt
actagaggccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctg
gttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggat
tcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctat
cctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaa
aaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaatactagagaaagaggag
aaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatg
ggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactac
tggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagat
catatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatg
acgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattga
ttttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagac
aaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatt
atcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttc
gaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggat
gaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatac
tagaggccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggt
tggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattc
ccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcc
tgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaa
ggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaatactagagaaagaggagaa
atactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggt
cacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaag
gtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggc
tgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggt
gttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcc
cgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgc
tctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatg
gctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactaca
ccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatc
gctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata