LOCUS BBa_K545000 3624 bp DNA linear 15-Nov-2024 DEFINITION pCin + CrtEBI (lycopene enzymes) ACCESSION BBa_K545000 VERSION BBa_K545000.1 FEATURES Location/Qualifiers RBS 226..231 /label=embedded RBS stem_loop 35..74 /label=stem_loop stem_loop 91..136 /label=stem_loop promoter 1..220 /label=unidentified/uncharacterized CinR dependent promoter 1..231 /label=BBa_R0077 RBS 240..243 /label=BBa_K118012 CDS 250..1161 /label=BBa_I742151 misc_feature 1170..1172 /label=SacI RBS 1173..1179 /label=rbs RBS 1170..1179 /label=BBa_J15001 CDS 1186..2115 /label=crtB coding sequence CDS 1186..2118 /label=BBa_K118002 misc_feature 2127..2129 /label=SacI RBS 2130..2136 /label=rbs RBS 2127..2136 /label=BBa_J15001 misc_feature 2620..2625 /label=PstI site removed misc_feature 3031..3036 /label=PstI site removed CDS 2143..3621 /label=crtI coding sequence CDS 2143..3624 /label=BBa_K118003 misc_feature 240..3624 /label=BBa_K274100 ORIGIN 1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc 61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac 121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga 181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctggag gtactagagg 241 aggtactaga tgacggtctg cgcaaaaaaa cacgttcatc tcactcgcga tgctgcggag 301 cagttactgg ctgatattga tcgacgcctt gatcagttat tgcccgtgga gggagaacgg 361 gatgttgtgg gtgccgcgat gcgtgaaggt gcgctggcac cgggaaaacg tattcgcccc 421 atgttgctgt tgctgaccgc ccgcgatctg ggttgcgctg tcagccatga cggattactg 481 gatttggcct gtgcggtgga aatggtccac gcggcttcgc tgatccttga cgatatgccc 541 tgcatggacg atgcgaagct gcggcgcgga cgccctacca ttcattctca ttacggagag 601 catgtggcaa tactggcggc ggttgccttg ctgagtaaag cctttggcgt aattgccgat 661 gcagatggcc tcacgccgct ggcaaaaaat cgggcggttt ctgaactgtc aaacgccatc 721 ggcatgcaag gattggttca gggtcagttc aaggatctgt ctgaagggga taagccgcgc 781 agcgctgaag ctattttgat gacgaatcac tttaaaacca gcacgctgtt ttgtgcctcc 841 atgcagatgg cctcgattgt tgcgaatgcc tccagcgaag cgcgtgattg cctgcatcgt 901 ttttcacttg atcttggtca ggcatttcaa ctgctggacg atttgaccga tggcatgacc 961 gacaccggta aggatagcaa tcaggacgcc ggtaaatcga cgctggtcaa tctgttaggc 1021 ccgagggcgg ttgaagaacg tctgagacaa catcttcagc ttgccagtga gcatctctct 1081 gcggcctgcc aacacgggca cgccactcaa cattttattc aggcctggtt tgacaaaaaa 1141 ctcgctgccg tcagttaata atactagagc tcaaggaggt actagatgaa taatccgtcg 1201 ttactcaatc atgcggtcga aacgatggca gttggctcga aaagttttgc gacagcctca 1261 aagttatttg atgcaaaaac ccggcgcagc gtactgatgc tctacgcctg gtgccgccat 1321 tgtgacgatg ttattgacga tcagacgctg ggctttcagg cccggcagcc tgccttacaa 1381 acgcccgaac aacgtctgat gcaacttgag atgaaaacgc gccaggccta tgcaggatcg 1441 cagatgcacg aaccggcgtt tgcggctttt caggaagtgg ctatggctca tgatatcgcc 1501 ccggcttacg cgtttgatca tctggaaggc ttcgccatgg atgtacgcga agcgcaatac 1561 agccaactgg atgatacgct gcgctattgc tatcacgttg caggcgttgt cggcttgatg 1621 atggcgcaaa tcatgggcgt gcgggataac gccacgctgg accgcgcctg tgaccttggg 1681 ctggcatttc agttgaccaa tattgctcgc gatattgtgg acgatgcgca tgcgggccgc 1741 tgttatctgc cggcaagctg gctggagcat gaaggtctga acaaagagaa ttatgcggca 1801 cctgaaaacc gtcaggcgct gagccgtatc gcccgtcgtt tggtgcagga agcagaacct 1861 tactatttgt ctgccacagc cggcctggca gggttgcccc tgcgttccgc ctgggcaatc 1921 gctacggcga agcaggttta ccggaaaata ggtgtcaaag ttgaacaggc cggtcagcaa 1981 gcctgggatc agcggcagtc aacgaccacg cccgaaaaat taacgctgct gctggccgcc 2041 tctggtcagg cccttacttc ccggatgcgg gctcatcctc cccgccctgc gcatctctgg 2101 cagcgcccgc tctaataata ctagagctca aggaggtact agatgaaacc aactacggta 2161 attggtgcag gcttcggtgg cctggcactg gcaattcgtc tacaagctgc ggggatcccc 2221 gtcttactgc ttgaacaacg tgataaaccc ggcggtcggg cttatgtcta cgaggatcag 2281 gggtttacct ttgatgcagg cccgacggtt atcaccgatc ccagtgccat tgaagaactg 2341 tttgcactgg caggaaaaca gttaaaagag tatgtcgaac tgctgccggt tacgccgttt 2401 taccgcctgt gttgggagtc agggaaggtc tttaattacg ataacgatca aacccggctc 2461 gaagcgcaga ttcagcagtt taatccccgc gatgtcgaag gttatcgtca gtttctggac 2521 tattcacgcg cggtgtttaa agaaggctat ctaaagctcg gtactgtccc ttttttatcg 2581 ttcagagaca tgcttcgcgc cgcacctcaa ctggcgaaac tgcaagcatg gagaagcgtt 2641 tacagtaagg ttgccagtta catcgaagat gaacatctgc gccaggcgtt ttctttccac 2701 tcgctgttgg tgggcggcaa tcccttcgcc acctcatcca tttatacgtt gatacacgcg 2761 ctggagcgtg agtggggcgt ctggtttccg cgtggcggca ccggcgcatt agttcagggg 2821 atgataaagc tgtttcagga tctgggtggc gaagtcgtgt taaacgccag agtcagccat 2881 atggaaacga caggaaacaa gattgaagcc gtgcatttag aggacggtcg caggttcctg 2941 acgcaagccg tcgcgtcaaa tgcagatgtg gttcatacct atcgcgacct gttaagccag 3001 caccctgccg cggttaagca gtccaacaaa ctgcaaacta agcgcatgag taactctctg 3061 tttgtgctct attttggttt gaatcaccat catgatcagc tcgcgcatca cacggtttgt 3121 ttcggcccgc gttaccgcga gctgattgac gaaattttta atcatgatgg cctcgcagag 3181 gacttctcac tttatctgca cgcgccctgt gtcacggatt cgtcactggc gcctgaaggt 3241 tgcggcagtt actatgtgtt ggcgccggtg ccgcatttag gcaccgcgaa cctcgactgg 3301 acggttgagg ggccaaaact acgcgaccgt atttttgcgt accttgagca gcattacatg 3361 cctggcttac ggagtcagct ggtcacgcac cggatgttta cgccgtttga ttttcgcgac 3421 cagcttaatg cctatcatgg ctcagccttt tctgtggagc ccgttcttac ccagagcgcc 3481 tggtttcggc cgcataaccg cgataaaacc attactaatc tctacctggt cggcgcaggc 3541 acgcatcccg gcgcaggcat tcctggcgtc atcggctcgg caaaagcgac agcaggtttg 3601 atgctggagg atctgatata ataa //