LOCUS       BBa_K545003             2645 bp    DNA     linear       29-Nov-2024
DEFINITION  LacI repressor followed by pLac promoter regulating MerR and CinR
ACCESSION   BBa_K545003
VERSION     BBa_K545003.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     terminator      1180..1308
                     /label=BBa_B0015
     misc_feature    1319..1335
                     /label=lac O1
     promoter        1336..1341
                     /label=-35
     misc_feature    1342..1358
                     /label=lac O1
     promoter        1359..1364
                     /label=-10
     misc_feature    1317..1370
                     /label=BBa_R0011
     promoter        1317..1370
                     /label=BBa_R0011
     misc_feature    1..1371
                     /label=BBa_Q04121
     RBS             1380..1391
                     /label=B0034
     CDS             1398..1832
                     /label=merR
     misc_feature    1380..1832
                     /label=BBa_K346001
     misc_feature    1845..1848
                     /label=conserved
     RBS             1841..1852
                     /label=BBa_B0034
     CDS             1859..2581
                     /label=cinR
     misc_feature    2582..2614
                     /label=LVA
     misc_feature    2621..2645
                     /label=Help:Barcodes
     CDS             1859..2645
                     /label=BBa_C0077
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt
     1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga
     1381 aagaggagaa atactagatg gaaaataatt tggaaaacct gaccattggc gtttttgcca
     1441 aggcggccgg ggtcaacgtg gagacaatcc gcttctatca gcgcaagggc ctgttgcggg
     1501 aaccggacaa gccttacggc agcatccgcc gctatgggga ggcggacgtg gttcgggtga
     1561 aattcgtgaa atcggcacag cggctggggt tcagtctgga cgagattgcc gagctgttgc
     1621 ggctcgacga tggcacccac tgcgaggagg ccagcagcct ggccgaacac aagctcaagg
     1681 acgtgcgcga gaagatggcc gacttggcgc gcatggaaac cgtgctgtct gaactcgtgt
     1741 gcgcctgcca tgcacgaaag gggaatgttt cctgcccgtt gatcgcgtca ctacagggcg
     1801 aagcaggcct ggcaaggtca gctatgcctt agtactagag aaagaggaga aatactagat
     1861 gattgagaat acctatagcg aaaagttcga gtccgcgttc gaacagatca aggcggcggc
     1921 caacgtggat gccgccatcc gtattctcca ggcggaatat aacctcgatt tcgtcaccta
     1981 ccatctcgcc cagacgatcg cgagcaagat cgattcgccc ttcgtgcgca ccacctatcc
     2041 ggatgcctgg gtttcccgct acctcctcaa cagctatgtg aaggtcgatc cgatcgtcaa
     2101 gcagggcttc gaacgccagc tgcccttcga ctggagcgag gtcgaaccga cgccggaggc
     2161 ctatgccatg ctggtcgacg cccagaaaca cggcatcggt ggcaatggct actccatccc
     2221 cgtcgccgac aaggcgcagc gccgcgccct gctgtcgctg aatgcccgta taccggccga
     2281 cgaatggacc gagctcgtgc gccgctgccg caacgagtgg atcgagatcg cccatctgat
     2341 ccaccgcaag gccgtctatg agctgcatgg cgaaaacgat ccggtgccgg cattgtcgcc
     2401 gcgcgagatc gagtgtctgc actggaccgc cctcggcaag gattacaagg atatttcggt
     2461 catcctgggc atatcagagc ataccacacg cgattacctg aagaccgccc gcttcaagct
     2521 cggctgcgcc acgatctcgg ccgccgcgtc gcgggctgtt caattgcgca tcatcaatcc
     2581 cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag
     2641 atcgc
//