LOCUS BBa_K545042 3028 bp DNA linear 22-Nov-2024 DEFINITION Toggle Switch (LacI - TetR) which express GFP in one side ACCESSION BBa_K545042 VERSION BBa_K545042.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 terminator 1242..1370 /label=BBa_B0015 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 63..1433 /label=BBa_Q04121 misc_feature 1446..1449 /label=conserved RBS 1442..1453 /label=BBa_B0034 CDS 1463..2079 /label=tetR misc_feature 2080..2113 /label=SsrA misc_feature 2120..2144 /label=Help:Barcodes CDS 1460..2144 /label=BBa_C0040 RBS 2153..2165 /label=RBS-3\Weak RBS 2159..2162 /label=RBS misc_feature 2153..2165 /label=BBa_B0032 RBS 2153..2165 /label=BBa_B0032 CDS 2172..2891 /label=GFP protein CDS 2172..2891 /label=BBa_E0040 stem_loop 2911..2954 /label=stem_loop misc_feature 2900..2979 /label=BBa_B0010 terminator 2900..2979 /label=BBa_B0010 stem_loop 2995..3014 /label=T7 TE misc_feature 3021..3021 /label=stop polyA_site 3015..3028 /label=polya misc_feature 2988..3028 /label=BBa_B0012 terminator 2988..3028 /label=BBa_B0012 misc_feature 2153..3028 /label=BBa_E0240 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 1501 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 1561 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 1621 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 1681 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 1741 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 1801 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 1861 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 1921 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 1981 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 2041 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agtcacacag 2161 gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg 2221 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg 2281 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat 2341 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc 2401 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa 2461 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg 2521 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc 2581 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac 2641 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc 2701 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag 2761 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc 2821 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat 2881 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2941 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 3001 ccttcgggtg ggcctttctg cgtttata //