##gff-version 3
##sequence-region BBa_K545042 1 3028
BBa_K545042	.	ribosome_entry_site	1442	1453	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K545042	.	conserved_region	1446	1449	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K545042	.	engineered_region	63	1433	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_K545042	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_K545042	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K545042	.	terminator	1242	1370	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_K545042	.	terminator	1242	1321	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K545042	.	stem_loop	1253	1296	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K545042	.	engineered_region	1242	1321	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K545042	.	terminator	1330	1370	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K545042	.	polyA_site	1357	1370	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K545042	.	stop_codon	1363	1363	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K545042	.	engineered_region	1330	1370	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K545042	.	stem_loop	1337	1356	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K545042	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_K545042	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K545042	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K545042	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K545042	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K545042	.	promoter	1379	1432	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_K545042	.	promoter	1421	1426	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K545042	.	operator	1381	1397	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K545042	.	operator	1404	1420	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K545042	.	promoter	1398	1403	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K545042	.	engineered_region	1379	1432	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K545042	.	engineered_region	2153	3028	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K545042	.	ribosome_entry_site	2153	2165	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K545042	.	ribosome_entry_site	2153	2165	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K545042	.	ribosome_entry_site	2159	2162	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K545042	.	engineered_region	2153	2165	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K545042	.	CDS	2172	2891	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K545042	.	CDS	2172	2891	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K545042	.	terminator	2900	2979	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K545042	.	stem_loop	2911	2954	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K545042	.	engineered_region	2900	2979	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K545042	.	terminator	2988	3028	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K545042	.	polyA_site	3015	3028	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K545042	.	stop_codon	3021	3021	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K545042	.	engineered_region	2988	3028	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K545042	.	stem_loop	2995	3014	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K545042	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K545042	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K545042	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K545042	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K545042	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K545042	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K545042	.	CDS	1460	2144	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K545042	.	CDS	1463	2079	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K545042	.	tag	2080	2113	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K545042	.	engineered_tag	2120	2144	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_K545042
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaaga
tactgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcatta
gagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacat
tgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcactt
ttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcat
cgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttt
tatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtatt
ggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaa
gctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaa
aacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgcta
gtgtagatcactactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacgga
aaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatg
gtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgt
acaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccctt
gttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactata
actcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattga
agatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaac
cattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaa
cagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttata