LOCUS       BBa_K546000             1964 bp    DNA     linear       25-Nov-2024
DEFINITION  Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
ACCESSION   BBa_K546000
VERSION     BBa_K546000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1171..1174
                     /label=conserved
     RBS             1167..1178
                     /label=BBa_B0034
     misc_feature    1764..1795
                     /label=LVA
     CDS             1185..1763
                     /label=luxI
     misc_feature    1185..1802
                     /label=BBa_C0061
     misc_feature    1803..1827
                     /label=Help:Barcodes
     CDS             1185..1802
                     /label=BBa_C0061
     misc_feature    1167..1827
                     /label=BBa_C0261
     stem_loop       1847..1890
                     /label=stem_loop
     misc_feature    1836..1915
                     /label=BBa_B0010
     terminator      1836..1915
                     /label=BBa_B0010
     stem_loop       1931..1950
                     /label=T7 TE
     misc_feature    1957..1957
                     /label=stop
     polyA_site      1951..1964
                     /label=polya
     misc_feature    1924..1964
                     /label=BBa_B0012
     terminator      1924..1964
                     /label=BBa_B0012
     terminator      1836..1964
                     /label=BBa_B0015
     misc_feature    1167..1964
                     /label=BBa_K082014
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact ataatgataa
     1201 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt
     1261 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag
     1321 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa
     1381 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg
     1441 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg
     1501 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat
     1561 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat
     1621 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca
     1681 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac
     1741 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat
     1801 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc
     1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//