LOCUS       BBa_K546001             2135 bp    DNA     linear       14-May-2024
DEFINITION  Lux pL controlled luxR with lux pR controlled AHL degrading enzyme 
            AiiA(lva tag).
ACCESSION   BBa_K546001
VERSION     BBa_K546001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1171..1174
                     /label=conserved
     RBS             1167..1178
                     /label=BBa_B0034
     misc_feature    1185..1187
                     /label=start
     misc_feature    1968..1973
                     /label=2
     misc_feature    1935..1967
                     /label=LVA
     CDS             1185..1934
                     /label=aiiA
     misc_feature    1185..1973
                     /label=BBa_C0060
     misc_feature    1974..1998
                     /label=Help:Barcodes
     CDS             1185..1973
                     /label=BBa_C0060
     stem_loop       2018..2061
                     /label=stem_loop
     misc_feature    2007..2086
                     /label=BBa_B0010
     terminator      2007..2086
                     /label=BBa_B0010
     stem_loop       2102..2121
                     /label=T7 TE
     misc_feature    2128..2128
                     /label=stop
     polyA_site      2122..2135
                     /label=polya
     misc_feature    2095..2135
                     /label=BBa_B0012
     terminator      2095..2135
                     /label=BBa_B0012
     misc_feature    1167..2135
                     /label=BBa_I0460
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgaca gtaaagaagc
     1201 tttatttcgt cccagcaggt cgttgtatgt tggatcattc gtctgttaat agtacattaa
     1261 caccaggaga attattagac ttaccggttt ggtgttatct tttggagact gaagaaggac
     1321 ctattttagt agatacaggt atgccagaaa gtgcagttaa taatgaaggt ctttttaacg
     1381 gtacatttgt cgaagggcag gttttaccga aaatgactga agaagataga atcgtgaata
     1441 ttttaaaacg ggttggttat gagccggaag accttcttta tattattagt tctcacttgc
     1501 attttgatca tgcaggagga aatggcgctt ttataaatac accaatcatt gtacagcgtg
     1561 ctgaatatga ggcggcgcag catagcgaag aatatttgaa agaatgtata ttgccgaatt
     1621 taaactacaa aatcattgaa ggtgattatg aagtcgtacc aggagttcaa ttattgcata
     1681 caccaggcca tactccaggg catcaatcgc tattaattga gacagaaaaa tccggtcctg
     1741 tattattaac gattgatgca tcgtatacga aagagaattt tgaaaatgaa gtgccatttg
     1801 cgggatttga ttcagaatta gctttatctt caattaaacg tttaaaagaa gtggtgatga
     1861 aagagaagcc gattgttttc tttggacatg atatagagca ggaaagggga tgtaaagtgt
     1921 tccctgaata tatagctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat
     1981 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2101 tggctcacct tcgggtgggc ctttctgcgt ttata
//