LOCUS       BBa_K546002             2080 bp    DNA     linear       16-Feb-2025
DEFINITION  Lux pL controlled luxR with lux pR controlled GFP(lva tag).
ACCESSION   BBa_K546002
VERSION     BBa_K546002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1171..1174
                     /label=conserved
     RBS             1167..1178
                     /label=BBa_B0034
     misc_feature    1185..1904
                     /label=BBa_E0040
     misc_feature    1899..1937
                     /label=LVA
     misc_feature    1938..1943
                     /label=Double Stop
     misc_feature    1185..1187
                     /label=Start
     misc_feature    1185..1943
                     /label=BBa_J04031
     stem_loop       1963..2006
                     /label=stem_loop
     misc_feature    1952..2031
                     /label=BBa_B0010
     terminator      1952..2031
                     /label=BBa_B0010
     stem_loop       2047..2066
                     /label=T7 TE
     misc_feature    2073..2073
                     /label=stop
     polyA_site      2067..2080
                     /label=polya
     misc_feature    2040..2080
                     /label=BBa_B0012
     terminator      2040..2080
                     /label=BBa_B0012
     misc_feature    1167..2080
                     /label=BBa_I763020
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgcgt aaaggagaag
     1201 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     1261 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
     1321 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
     1381 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
     1441 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
     1501 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     1561 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     1621 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
     1681 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     1741 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     1801 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     1861 ctgctgggat tacacatggc atggatgaac tatacaaaag gcctgctgca aacgacgaaa
     1921 actacgcttt agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1981 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2041 cacactggct caccttcggg tgggcctttc tgcgtttata
//