LOCUS       BBa_K546004             2110 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive LacI and RFP expression
ACCESSION   BBa_K546004
VERSION     BBa_K546004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     misc_feature    63..1233
                     /label=BBa_S03520
     misc_feature    1246..1249
                     /label=conserved
     RBS             1242..1253
                     /label=BBa_B0034
     CDS             1260..1970
                     /label=mCherry
     misc_feature    1611..1611
                     /label=C->T (removing PstI site)
     misc_feature    1260..1973
                     /label=BBa_J06504
     stem_loop       1993..2036
                     /label=stem_loop
     misc_feature    1982..2061
                     /label=BBa_B0010
     terminator      1982..2061
                     /label=BBa_B0010
     stem_loop       2077..2096
                     /label=T7 TE
     misc_feature    2103..2103
                     /label=stop
     polyA_site      2097..2110
                     /label=polya
     misc_feature    2070..2110
                     /label=BBa_B0012
     terminator      2070..2110
                     /label=BBa_B0012
     misc_feature    1242..2110
                     /label=BBa_J06702
     misc_feature    63..2110
                     /label=BBa_J24675
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag aaatactaga
     1261 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     1321 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1381 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     1441 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     1501 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     1561 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg
     1621 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     1681 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     1741 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     1801 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     1861 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     1921 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa taatactaga
     1981 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2041 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2101 tgcgtttata
//