##gff-version 3
##sequence-region BBa_K546546 1 3909
BBa_K546546	.	CDS	2145	2762	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K546546	.	CDS	2145	2723	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K546546	.	engineered_tag	2763	2787	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K546546	.	engineered_region	2145	2762	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K546546	.	tag	2724	2755	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K546546	.	engineered_region	2996	3909	.	+	0	ID=BBa_I763020;Name=BBa_I763020
BBa_K546546	.	engineered_region	3014	3772	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_K546546	.	start_codon	3014	3016	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K546546	.	tag	3728	3766	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K546546	.	engineered_region	3014	3733	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K546546	.	stop_codon	3767	3772	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K546546	.	terminator	3781	3860	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_K546546	.	engineered_region	3781	3860	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K546546	.	stem_loop	3792	3835	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K546546	.	terminator	3869	3909	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_K546546	.	engineered_region	3869	3909	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K546546	.	stop_codon	3902	3902	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K546546	.	stem_loop	3876	3895	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K546546	.	polyA_site	3896	3909	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K546546	.	ribosome_entry_site	2996	3007	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_K546546	.	conserved_region	3000	3003	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K546546	.	promoter	2933	2987	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K546546	.	promoter	2974	2979	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K546546	.	engineered_region	2933	2987	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K546546	.	promoter	2952	2957	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K546546	.	start_codon	2985	2985	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K546546	.	non_covalent_binding_site	2933	2952	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K546546	.	promoter	2045	2138	.	+	0	ID=BBa_K182102;Name=BBa_K182102
BBa_K546546	.	ribosome_entry_site	2127	2138	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K182102
BBa_K546546	.	conserved_region	2131	2134	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K546546	.	promoter	2045	2118	.	+	0	ID=BBa_I751502;Name=BBa_I751502;Parent=BBa_K182102
BBa_K546546	.	promoter	2064	2118	.	+	0	ID=annotation1955559;Name=plac;Parent=BBa_I751502
BBa_K546546	.	sequence_feature	2064	2070	.	+	0	ID=annotation1955560;Name=-35;Parent=BBa_I751502
BBa_K546546	.	non_covalent_binding_site	2093	2118	.	+	0	ID=annotation1955563;Name=O1;Parent=BBa_I751502
BBa_K546546	.	non_covalent_binding_site	2071	2085	.	+	0	ID=annotation1955562;Name=O1;Parent=BBa_I751502
BBa_K546546	.	non_covalent_binding_site	2045	2063	.	+	0	ID=annotation1955564;Name=luxR binding site;Parent=BBa_I751502
BBa_K546546	.	sequence_feature	2086	2092	.	+	0	ID=annotation1955561;Name=-10;Parent=BBa_I751502
BBa_K546546	.	engineered_region	1068	2036	.	+	0	ID=BBa_I0460;Name=BBa_I0460
BBa_K546546	.	ribosome_entry_site	1068	1079	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_K546546	.	conserved_region	1072	1075	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K546546	.	terminator	1908	1987	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_K546546	.	engineered_region	1908	1987	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K546546	.	stem_loop	1919	1962	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K546546	.	terminator	1996	2036	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_K546546	.	engineered_region	1996	2036	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K546546	.	stop_codon	2029	2029	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K546546	.	stem_loop	2003	2022	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K546546	.	polyA_site	2023	2036	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K546546	.	CDS	1086	1874	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_K546546	.	engineered_region	1086	1874	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K546546	.	start_codon	1086	1088	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K546546	.	engineered_tag	1875	1899	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K546546	.	CDS	1086	1835	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K546546	.	tag	1836	1868	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K546546	.	stop_codon	1869	1874	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K546546	.	terminator	2796	2924	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K546546	.	terminator	2796	2875	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K546546	.	engineered_region	2796	2875	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K546546	.	stem_loop	2807	2850	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K546546	.	terminator	2884	2924	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K546546	.	engineered_region	2884	2924	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K546546	.	stop_codon	2917	2917	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K546546	.	stem_loop	2891	2910	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K546546	.	polyA_site	2911	2924	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K546546	.	engineered_region	1	1059	.	+	0	ID=BBa_K176028;Name=BBa_K176028
BBa_K546546	.	engineered_region	1	979	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176028
BBa_K546546	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K546546	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K546546	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K546546	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K546546	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K546546	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K546546	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K546546	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K546546	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K546546	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K546546	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K546546	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K546546	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K546546	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K546546	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K546546	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K546546	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K546546	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K546546	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K546546	.	promoter	988	1059	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176028
BBa_K546546	.	promoter	1007	1012	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K546546	.	non_covalent_binding_site	1041	1059	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K546546	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K546546	.	start_codon	1040	1040	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K546546	.	promoter	1029	1034	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K546546	.	engineered_region	988	1040	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
>BBa_K546546
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaatatccctatcagtgatagagatactagagaaagaggagaaatactagatgacagtaaagaagctttat
ttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttac
cggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataa
tgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatatt
ttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaa
atggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaa
agaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcat
acaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatg
catcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaa
acgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaa
gtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagat
cgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtagga
tcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatttactagagaaagagg
agaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaag
tcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgat
aactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacag
gtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaatt
aagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagct
atatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagc
gtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctat
gcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactc
tgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagagg
agaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaa
tgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcact
actggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccag
atcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaaga
tgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtatt
gattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacca
ttatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctt
tcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata