BBa_K546546
type: ComponentDefinition
persistent identity: BBa K546546
display id: BBa_K546546
version: 1
was generated by: iGEM to SBOL conversion
description: The Synchroscillator: A Tunable Synchronized Oscillatory System
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
mutable provenance: The lux pR promoter originates from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
mutable description: This BioBrick device yields an oscillating pattern of GFP (with an LVA Tag) expression. It contains a plux-lac hybrid promoter that enables fine tuning of the oscillation by LacI presence.
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Some parameters of the signalling molecule formation and degradation were unknown. Modeling of the BioBrick device was therefore hard. The addtion of extra promoters should enable stablization of the oscillation.
star: false
creator: M. Hesselman and B. Ryback
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K176028
component: BBa_K182102
component: BBa_I0460
component: BBa_B0015
component: BBa_C0061
component: BBa_I763020
component: BBa_R0062
sequence annotation: BBa_K176028
sequence annotation: BBa_I0460
sequence annotation: BBa_K182102
sequence annotation: BBa_C0061
sequence annotation: BBa_B0015
sequence annotation: BBa_R0062
sequence annotation: BBa_I763020
sequence: https://synbiohub.org/public/igem/BBa_K546546_sequence/1

BBa_K176028
type: Component
persistent identity: component2320870
display id: component2320870
version: 1
top level: BBa_K546546
definition: BBa_K176028
access: public

BBa_K182102
type: Component
persistent identity: component2320895
display id: component2320895
version: 1
top level: BBa_K546546
definition: BBa_K182102
access: public

BBa_I0460
type: Component
persistent identity: component2320885
display id: component2320885
version: 1
top level: BBa_K546546
definition: BBa_I0460
access: public

BBa_B0015
type: Component
persistent identity: component2320906
display id: component2320906
version: 1
top level: BBa_K546546
definition: BBa_B0015
access: public

BBa_C0061
type: Component
persistent identity: component2320897
display id: component2320897
version: 1
top level: BBa_K546546
definition: luxI
access: public

BBa_I763020
type: Component
persistent identity: component2320925
display id: component2320925
version: 1
top level: BBa_K546546
definition: RBS-GFP-Te
access: public

BBa_R0062
type: Component
persistent identity: component2320908
display id: component2320908
version: 1
top level: BBa_K546546
definition: lux pR
access: public

BBa_K176028
type: SequenceAnnotation
persistent identity: annotation2320870
display id: annotation2320870
version: 1
top level: BBa_K546546
location: https://synbiohub.org/public/igem/BBa_K546546/annotation2320870/range2320870/1
component: BBa_K176028

https://synbiohub.org/public/igem/BBa_K546546/annotation2320870/range2320870/1
type: Range
persistent identity: range2320870
display id: range2320870
version: 1
top level: BBa_K546546
direction: direction/reverse
start: 1
end: 1059
orientation: inline

BBa_I0460
type: SequenceAnnotation
persistent identity: annotation2320885
display id: annotation2320885
version: 1
top level: BBa_K546546
location: https://synbiohub.org/public/igem/BBa_K546546/annotation2320885/range2320885/1
component: BBa_I0460

https://synbiohub.org/public/igem/BBa_K546546/annotation2320885/range2320885/1
type: Range
persistent identity: range2320885
display id: range2320885
version: 1
top level: BBa_K546546
direction: direction/reverse
start: 1068
end: 2036
orientation: inline

BBa_K182102
type: SequenceAnnotation
persistent identity: annotation2320895
display id: annotation2320895
version: 1
top level: BBa_K546546
location: https://synbiohub.org/public/igem/BBa_K546546/annotation2320895/range2320895/1
component: BBa_K182102

https://synbiohub.org/public/igem/BBa_K546546/annotation2320895/range2320895/1
type: Range
persistent identity: range2320895
display id: range2320895
version: 1
top level: BBa_K546546
direction: direction/reverse
start: 2045
end: 2138
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2320897
display id: annotation2320897
version: 1
top level: BBa_K546546
location: https://synbiohub.org/public/igem/BBa_K546546/annotation2320897/range2320897/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K546546/annotation2320897/range2320897/1
type: Range
persistent identity: range2320897
display id: range2320897
version: 1
top level: BBa_K546546
direction: direction/forward
start: 2145
end: 2762
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2320906
display id: annotation2320906
version: 1
top level: BBa_K546546
location: https://synbiohub.org/public/igem/BBa_K546546/annotation2320906/range2320906/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K546546/annotation2320906/range2320906/1
type: Range
persistent identity: range2320906
display id: range2320906
version: 1
top level: BBa_K546546
direction: direction/reverse
start: 2796
end: 2924
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2320908
display id: annotation2320908
version: 1
top level: BBa_K546546
location: https://synbiohub.org/public/igem/BBa_K546546/annotation2320908/range2320908/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K546546/annotation2320908/range2320908/1
type: Range
persistent identity: range2320908
display id: range2320908
version: 1
top level: BBa_K546546
direction: direction/forward
start: 2933
end: 2987
orientation: inline

BBa_I763020
type: SequenceAnnotation
persistent identity: annotation2320925
display id: annotation2320925
version: 1
top level: BBa_K546546
location: https://synbiohub.org/public/igem/BBa_K546546/annotation2320925/range2320925/1
component: BBa_I763020

https://synbiohub.org/public/igem/BBa_K546546/annotation2320925/range2320925/1
type: Range
persistent identity: range2320925
display id: range2320925
version: 1
top level: BBa_K546546
direction: direction/reverse
start: 2996
end: 3909
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I0460
type: ComponentDefinition
persistent identity: BBa I0460
display id: BBa_I0460
version: 1
was generated by: iGEM to SBOL conversion
description: aiiA Device (B0034.C0060.B0015)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: true
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_C0060
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0060
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0460_sequence/1

BBa_B0012
type: Component
persistent identity: component943127
display id: component943127
version: 1
top level: BBa_I0460
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component943117
display id: component943117
version: 1
top level: BBa_I0460
definition: BBa_B0010
access: public

BBa_C0060
type: Component
persistent identity: component943109
display id: component943109
version: 1
top level: BBa_I0460
definition: aiiA
access: public

BBa_B0034
type: Component
persistent identity: component943094
display id: component943094
version: 1
top level: BBa_I0460
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943094
display id: annotation943094
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943094/range943094/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0460/annotation943094/range943094/1
type: Range
persistent identity: range943094
display id: range943094
version: 1
top level: BBa_I0460
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation943109
display id: annotation943109
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943109/range943109/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I0460/annotation943109/range943109/1
type: Range
persistent identity: range943109
display id: range943109
version: 1
top level: BBa_I0460
direction: direction/forward
start: 19
end: 807
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943117
display id: annotation943117
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943117/range943117/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0460/annotation943117/range943117/1
type: Range
persistent identity: range943117
display id: range943117
version: 1
top level: BBa_I0460
direction: direction/forward
start: 841
end: 920
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943127
display id: annotation943127
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943127/range943127/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0460/annotation943127/range943127/1
type: Range
persistent identity: range943127
display id: range943127
version: 1
top level: BBa_I0460
direction: direction/forward
start: 929
end: 969
orientation: inline

RBS-GFP-Te
type: ComponentDefinition
persistent identity: BBa I763020
display id: BBa_I763020
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-GFP (+LVA Tag)-Terminators
created: 2007-08-26T11:00:00Z
modified: 2015-08-31T04:08:08Z
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Intermediate part composed by RBS (B0034), GFP with LVA tag and terminators.
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Intermediate
star: false
creator: Alice Pasini
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J04031
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J04031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I763020_sequence/1

BBa_J04031
type: Component
persistent identity: component1942525
display id: component1942525
version: 1
top level: RBS-GFP-Te
definition: BBa_J04031
access: public

BBa_B0034
type: Component
persistent identity: component1942520
display id: component1942520
version: 1
top level: RBS-GFP-Te
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1942528
display id: component1942528
version: 1
top level: RBS-GFP-Te
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1942526
display id: component1942526
version: 1
top level: RBS-GFP-Te
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1942520
display id: annotation1942520
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942520/range1942520/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I763020/annotation1942520/range1942520/1
type: Range
persistent identity: range1942520
display id: range1942520
version: 1
top level: RBS-GFP-Te
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation1942525
display id: annotation1942525
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942525/range1942525/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_I763020/annotation1942525/range1942525/1
type: Range
persistent identity: range1942525
display id: range1942525
version: 1
top level: RBS-GFP-Te
direction: direction/reverse
start: 19
end: 777
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1942526
display id: annotation1942526
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942526/range1942526/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I763020/annotation1942526/range1942526/1
type: Range
persistent identity: range1942526
display id: range1942526
version: 1
top level: RBS-GFP-Te
direction: direction/forward
start: 786
end: 865
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1942528
display id: annotation1942528
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942528/range1942528/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I763020/annotation1942528/range1942528/1
type: Range
persistent identity: range1942528
display id: range1942528
version: 1
top level: RBS-GFP-Te
direction: direction/forward
start: 874
end: 914
orientation: inline

BBa_K182102
type: ComponentDefinition
persistent identity: BBa K182102
display id: BBa_K182102
version: 1
was generated by: iGEM to SBOL conversion
description: pLux-Lac with RBS attached
created: 2009-07-06T11:00:00Z
modified: 2015-05-08T01:11:06Z
mutable provenance: BBa_I751502 and BBa_B0034)
owned by: james
owned by: myers
mutable description: pLux-Lac hybrid promoter (BBa_I751502) upstream with RBS (BBa_B0034) attached downstream.
discontinued: false
dominant: false
experience: experience/None
group u list: _279_
m user id: 0
owner id: 4115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: N/A atm
star: false
creator: Calum de Burgh and Zuzana Hruskova
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_B0034
component: BBa_I751502
sequence annotation: BBa_I751502
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K182102_sequence/1

BBa_B0034
type: Component
persistent identity: component2010165
display id: component2010165
version: 1
top level: BBa_K182102
definition: BBa_B0034
access: public

BBa_I751502
type: Component
persistent identity: component2010163
display id: component2010163
version: 1
top level: BBa_K182102
definition: BBa_I751502
access: public

BBa_I751502
type: SequenceAnnotation
persistent identity: annotation2010163
display id: annotation2010163
version: 1
top level: BBa_K182102
location: https://synbiohub.org/public/igem/BBa_K182102/annotation2010163/range2010163/1
component: BBa_I751502

https://synbiohub.org/public/igem/BBa_K182102/annotation2010163/range2010163/1
type: Range
persistent identity: range2010163
display id: range2010163
version: 1
top level: BBa_K182102
direction: direction/reverse
start: 1
end: 74
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2010165
display id: annotation2010165
version: 1
top level: BBa_K182102
location: https://synbiohub.org/public/igem/BBa_K182102/annotation2010165/range2010165/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K182102/annotation2010165/range2010165/1
type: Range
persistent identity: range2010165
display id: range2010165
version: 1
top level: BBa_K182102
direction: direction/reverse
start: 83
end: 94
orientation: inline

BBa_K176028
type: ComponentDefinition
persistent identity: BBa K176028
display id: BBa_K176028
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible AHL->PoPS Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->PoPS
created: 2009-07-19T11:00:00Z
modified: 2015-05-08T01:11:02Z
mutable provenance: K176025 K176000
owned by: james
owned by: myers
mutable description: AHL -> PoPS Receiver TetR repressible Similar to F2620, but use pLux/Tet hybrid promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Input: AHL Output: PoPS Design: pCon 0.70(J23101) generate LuxR pLux/Tet(K176000) generate PoPS output, activated by LuxR and AHL, repressed by TetR and no aTc
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K176000
component: BBa_K176025
sequence annotation: BBa_K176025
sequence annotation: BBa_K176000
sequence: https://synbiohub.org/public/igem/BBa_K176028_sequence/1

BBa_K176000
type: Component
persistent identity: component2262163
display id: component2262163
version: 1
top level: BBa_K176028
definition: pLux/Tet
access: public

BBa_K176025
type: Component
persistent identity: component2262156
display id: component2262156
version: 1
top level: BBa_K176028
definition: LuxR
access: public

BBa_K176025
type: SequenceAnnotation
persistent identity: annotation2262156
display id: annotation2262156
version: 1
top level: BBa_K176028
location: https://synbiohub.org/public/igem/BBa_K176028/annotation2262156/range2262156/1
component: BBa_K176025

https://synbiohub.org/public/igem/BBa_K176028/annotation2262156/range2262156/1
type: Range
persistent identity: range2262156
display id: range2262156
version: 1
top level: BBa_K176028
direction: direction/reverse
start: 1
end: 979
orientation: inline

BBa_K176000
type: SequenceAnnotation
persistent identity: annotation2262163
display id: annotation2262163
version: 1
top level: BBa_K176028
location: https://synbiohub.org/public/igem/BBa_K176028/annotation2262163/range2262163/1
component: BBa_K176000

https://synbiohub.org/public/igem/BBa_K176028/annotation2262163/range2262163/1
type: Range
persistent identity: range2262163
display id: range2262163
version: 1
top level: BBa_K176028
direction: direction/reverse
start: 988
end: 1059
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: <genbank>AF196486</genbank> from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: aiiA
sequence annotation: BBa_C0060
sequence annotation: start
sequence annotation: LVA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 750
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 789
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
top level: aiiA
direction: direction/forward
start: 751
end: 783
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
top level: aiiA
direction: direction/forward
start: 784
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
top level: aiiA
direction: direction/forward
start: 790
end: 814
orientation: inline

BBa_J04031
type: ComponentDefinition
persistent identity: BBa J04031
display id: BBa_J04031
version: 1
was generated by: iGEM to SBOL conversion
description: GFP w/LVA tag
created: 2005-06-07T11:00:00Z
modified: 2015-08-31T04:08:13Z
mutable provenance: Davidson Synth Aces
owned by: james
owned by: myers
mutable description: BBa_E0040 with an LVA tag added via PCR. BBa_E0040 is GFP(mut3b).
discontinued: false
dominant: true
experience: experience/None
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0040
sequence annotation: Start
sequence annotation: LVA
sequence annotation: Double Stop
sequence: https://synbiohub.org/public/igem/BBa_J04031_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1507202
display id: annotation1507202
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507202/range1507202/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J04031/annotation1507202/range1507202/1
type: Range
persistent identity: range1507202
display id: range1507202
version: 1
top level: BBa_J04031
direction: direction/forward
start: 1
end: 720
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation1507246
display id: annotation1507246
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507246/range1507246/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J04031/annotation1507246/range1507246/1
type: Range
persistent identity: range1507246
display id: range1507246
version: 1
top level: BBa_J04031
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1507235
display id: annotation1507235
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507235/range1507235/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J04031/annotation1507235/range1507235/1
type: Range
persistent identity: range1507235
display id: range1507235
version: 1
top level: BBa_J04031
direction: direction/forward
start: 715
end: 753
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation1507242
display id: annotation1507242
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507242/range1507242/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J04031/annotation1507242/range1507242/1
type: Range
persistent identity: range1507242
display id: range1507242
version: 1
top level: BBa_J04031
direction: direction/forward
start: 754
end: 759
orientation: inline

BBa_I751502
type: ComponentDefinition
persistent identity: BBa I751502
display id: BBa_I751502
version: 1
was generated by: iGEM to SBOL conversion
description: plux-lac hybrid promoter
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
star: false
creator: Kenichiro Iwasaki
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: luxR binding site
sequence annotation: plac
sequence annotation: -35
sequence annotation: O1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I751502_sequence/1

luxR binding site
type: SequenceAnnotation
persistent identity: annotation1955564
display id: annotation1955564
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
type: Range
persistent identity: range1955564
display id: range1955564
version: 1
top level: BBa_I751502
direction: direction/forward
start: 1
end: 19
orientation: inline

plac
type: SequenceAnnotation
persistent identity: annotation1955559
display id: annotation1955559
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
type: Range
persistent identity: range1955559
display id: range1955559
version: 1
top level: BBa_I751502
direction: direction/forward
start: 20
end: 74
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955560
display id: annotation1955560
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
type: Range
persistent identity: range1955560
display id: range1955560
version: 1
top level: BBa_I751502
direction: direction/forward
start: 20
end: 26
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955562
display id: annotation1955562
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
type: Range
persistent identity: range1955562
display id: range1955562
version: 1
top level: BBa_I751502
direction: direction/forward
start: 27
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955561
display id: annotation1955561
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
type: Range
persistent identity: range1955561
display id: range1955561
version: 1
top level: BBa_I751502
direction: direction/forward
start: 42
end: 48
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955563
display id: annotation1955563
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
type: Range
persistent identity: range1955563
display id: range1955563
version: 1
top level: BBa_I751502
direction: direction/forward
start: 49
end: 74
orientation: inline

pLux/Tet
type: ComponentDefinition
persistent identity: BBa K176000
display id: BBa_K176000
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS
created: 2009-05-05T11:00:00Z
modified: 2015-05-08T01:11:00Z
mutable provenance: Tet Operator sequence from BBa_R0040
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by tetR
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 4010
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: How to arrange the two parts together?
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: luxR/HSL
sequence annotation: R0062
sequence annotation: -35
sequence annotation: -10
sequence annotation: +1
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K176000_sequence/1

luxR/HSL
type: SequenceAnnotation
persistent identity: annotation2002612
display id: annotation2002612
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
type: Range
persistent identity: range2002612
display id: range2002612
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 20
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2002607
display id: annotation2002607
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
type: Range
persistent identity: range2002607
display id: range2002607
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002608
display id: annotation2002608
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
type: Range
persistent identity: range2002608
display id: range2002608
version: 1
top level: pLux/Tet
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002609
display id: annotation2002609
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
type: Range
persistent identity: range2002609
display id: range2002609
version: 1
top level: pLux/Tet
direction: direction/forward
start: 42
end: 47
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation2002611
display id: annotation2002611
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
type: Range
persistent identity: range2002611
display id: range2002611
version: 1
top level: pLux/Tet
direction: direction/forward
start: 53
end: 53
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2002610
display id: annotation2002610
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
type: Range
persistent identity: range2002610
display id: range2002610
version: 1
top level: pLux/Tet
direction: direction/forward
start: 54
end: 72
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa K176025
display id: BBa_K176025
version: 1
was generated by: iGEM to SBOL conversion
description: pCon 0.70(J23101)->RBS+luxR+T
created: 2009-07-15T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: J23101 I0462
owned by: james
owned by: myers
mutable description: pCon 0.70(J23101)+luxR(I0462)
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: pCon 0.70(J23101) generate LuxR
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23101
component: BBa_I0462
sequence annotation: BBa_J23101
sequence annotation: BBa_I0462
sequence: https://synbiohub.org/public/igem/BBa_K176025_sequence/1

BBa_J23101
type: Component
persistent identity: component2255337
display id: component2255337
version: 1
top level: LuxR
definition: BBa_J23101
access: public

BBa_I0462
type: Component
persistent identity: component2255352
display id: component2255352
version: 1
top level: LuxR
definition: LuxR
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2255337
display id: annotation2255337
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_K176025/annotation2255337/range2255337/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K176025/annotation2255337/range2255337/1
type: Range
persistent identity: range2255337
display id: range2255337
version: 1
top level: LuxR
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2255352
display id: annotation2255352
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_K176025/annotation2255352/range2255352/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_K176025/annotation2255352/range2255352/1
type: Range
persistent identity: range2255352
display id: range2255352
version: 1
top level: LuxR
direction: direction/reverse
start: 44
end: 979
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: iGEM to SBOL conversion
description: luxR Protein Generator
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
top level: LuxR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
top level: LuxR
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
top level: LuxR
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
top level: LuxR
direction: direction/forward
start: 896
end: 936
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0062
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

https://synbiohub.org/public/igem/BBa_K546546_sequence/1
type: Sequence
persistent identity: BBa K546546 sequence
display id: BBa_K546546_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0460_sequence/1
type: Sequence
persistent identity: BBa I0460 sequence
display id: BBa_I0460_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I763020_sequence/1
type: Sequence
persistent identity: BBa I763020 sequence
display id: BBa_I763020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K182102_sequence/1
type: Sequence
persistent identity: BBa K182102 sequence
display id: BBa_K182102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatttactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176028_sequence/1
type: Sequence
persistent identity: BBa K176028 sequence
display id: BBa_K176028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0060_sequence/1
type: Sequence
persistent identity: BBa C0060 sequence
display id: BBa_C0060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04031_sequence/1
type: Sequence
persistent identity: BBa J04031 sequence
display id: BBa_J04031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I751502_sequence/1
type: Sequence
persistent identity: BBa I751502 sequence
display id: BBa_I751502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176000_sequence/1
type: Sequence
persistent identity: BBa K176000 sequence
display id: BBa_K176000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176025_sequence/1
type: Sequence
persistent identity: BBa K176025 sequence
display id: BBa_K176025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0462_sequence/1
type: Sequence
persistent identity: BBa I0462 sequence
display id: BBa_I0462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z