LOCUS BBa_K547000 3735 bp DNA linear 03-May-2024 DEFINITION ready-to-inject backbone for T3SS, SlrP taged, with Bsa I cloning site ACCESSION BBa_K547000 VERSION BBa_K547000.1 FEATURES Location/Qualifiers misc_feature 8..136 /label=BBa_B0015 misc_feature 137..157 /label=Biobrick Suffix stem_loop 158..229 /label=E. coli his operon terminator stem_loop 166..200 /label=Stem loop misc_feature 397..1011 /label=rep (pMB1) CDS 1233..2048 /label=Kanamycin resistance marker misc_feature 2016..2016 /label=G ->C Silent mutation misc_feature 2153..2196 /label=repeat regeion stem_loop 2279..2282 /label=End of terminator stem_loop 2292..2311 /label=Stem loop misc_feature 2319..2340 /label=Biobrick Prefix misc_feature 2341..2394 /label=BBa_R0040 misc_feature 2402..2988 /label=BBa_K496003 misc_feature 2402..2413 /label=BBa_B0034 misc_feature 2416..2988 /label=SlrP CDS 2416..3729 /label=mat_peputide misc_feature 2586..2586 /label=T -> A Silent mutation misc_feature 2991..3001 /label=BsaI Cloning Site misc_feature 3010..3729 /label=BBa_E0040 misc_feature 3732..3735 /label=BsaI Cloning Site misc_feature 1..7 /label=BsaI Cloning Site misc_feature 157..2319 /label=pSB1K3 ORIGIN 1 agagacccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 61 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 121 cctttctgcg tttatatact agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt 181 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct 241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc 421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 1081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1141 gtatatatga gtaaacttgg tctgacagct cgagtcccgt caagtcagcg taatgctctg 1201 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa 1261 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa 1321 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc 1381 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt 1441 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg 1501 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc 1561 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct 1621 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc 1681 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc 1741 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt 1801 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt 1861 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa 1921 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa 1981 atcagcatcc atgttggaat ttaatcgcgg cctggagcaa gacgtttccc gttgaatatg 2041 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga 2101 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg 2161 aataaatcga acttttgctg agttgaagga tcagctcgag tgccacctga cgtctaagaa 2221 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga 2281 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag 2341 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 2401 aaaagaggag aaaatatgtt taatattact aatatacaat ctacggcaag gcatcaaagt 2461 attagcaatg aggcctcaac agaggtgcct ttaaaagaag agatatggaa taaaataagt 2521 gcctttttct cttcagaaca tcaggttgaa gcacaaaact gcatcgctta tctttgtcat 2581 ccacctgaaa ccgcctcgcc agaagagatc aaaagcaagt ttgaatgttt aaggatgtta 2641 gctttcccgg cgtatgcgga taatattcag tatagtagag gaggggcaga ccaatactgt 2701 attttgagtg aaaatagtca ggaaattctg tctatagttt ttaatacaga gggctatacc 2761 gttgagggag ggggaaagtc agtcacctat acccgtgtga cagaaagcga gcaggcgagt 2821 agcgcttccg gctccaaaga tgctgtgaat tatgagttaa tctggtctga gtgggtaaaa 2881 gaggcgccag cgaaagaggc agcaaatcgt gaagaagccg tacaacggat gcgtgactgc 2941 ctgaaaaata ataagacgga acttcgtctg aaaatattag gacttaccgg ggtctcaggc 3001 cgcttcgaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 3061 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 3121 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 3181 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 3241 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 3301 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 3361 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 3421 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 3481 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 3541 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 3601 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 3661 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 3721 aaataataat actag //