##gff-version 3
##sequence-region BBa_K547000 1 3735
BBa_K547000	.	sequence_feature	3732	3735	.	+	0	ID=annotation2155720;Name=BsaI Cloning Site
BBa_K547000	.	stem_loop	158	229	.	+	0	ID=annotation2155678;Name=E. coli his operon terminator
BBa_K547000	.	point_mutation	2016	2016	.	+	0	ID=annotation2155684;Name=G ->C Silent mutation
BBa_K547000	.	engineered_region	8	136	.	+	0	ID=annotation2155676;Name=BBa_B0015
BBa_K547000	.	sequence_feature	2991	3001	.	+	0	ID=annotation2155718;Name=BsaI Cloning Site
BBa_K547000	.	stem_loop	2279	2282	.	+	0	ID=annotation2155688;Name=End of terminator
BBa_K547000	.	engineered_region	3010	3729	.	+	0	ID=annotation2155719;Name=BBa_E0040
BBa_K547000	.	CDS	1233	2048	.	+	0	ID=annotation2155681;Name=Kanamycin resistance marker
BBa_K547000	.	engineered_region	2341	2394	.	+	0	ID=annotation2155691;Name=BBa_R0040
BBa_K547000	.	engineered_region	157	2319	.	+	0	ID=annotation2155722;Name=pSB1K3
BBa_K547000	.	CDS	2416	3729	.	+	0	ID=annotation2155716;Name=mat_peputide
BBa_K547000	.	sequence_feature	2319	2340	.	+	0	ID=annotation2155690;Name=Biobrick Prefix
BBa_K547000	.	stem_loop	2292	2311	.	+	0	ID=annotation2155689;Name=Stem loop
BBa_K547000	.	sequence_feature	2416	2988	.	+	0	ID=annotation2155715;Name=SlrP
BBa_K547000	.	sequence_feature	137	157	.	+	0	ID=annotation2155677;Name=Biobrick Suffix
BBa_K547000	.	point_mutation	2586	2586	.	+	0	ID=annotation2155717;Name=T -> A Silent mutation
BBa_K547000	.	sequence_feature	397	1011	.	+	0	ID=annotation2155680;Name=rep (pMB1)
BBa_K547000	.	sequence_feature	2153	2196	.	+	0	ID=annotation2155685;Name=repeat regeion
BBa_K547000	.	engineered_region	2402	2413	.	+	0	ID=annotation2155693;Name=BBa_B0034
BBa_K547000	.	stem_loop	166	200	.	+	0	ID=annotation2155679;Name=Stem loop
BBa_K547000	.	engineered_region	2402	2988	.	+	0	ID=annotation2155692;Name=BBa_K496003
BBa_K547000	.	sequence_feature	1	7	.	+	0	ID=annotation2155721;Name=BsaI Cloning Site
>BBa_K547000
agagaccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagt
ccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggctt
cctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggtt
atccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggcc
gcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaa
acccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgct
taccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcg
gtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaact
atcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgag
gtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgc
gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtg
gtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtc
tgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctt
ttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagt
cagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgc
aatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggc
agttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttccc
ctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgc
atttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattca
ttcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccg
gcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttc
ccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaatt
ccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactc
tggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttataccca
tataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacacccc
ttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagag
attttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacg
tctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatc
cttagctttcgctaaggatgatttctggaattcgcggccgcttctagagtccctatcagtgatagagattgacatccct
atcagtgatagagatactgagcactactagaaaagaggagaaaatatgtttaatattactaatatacaatctacggcaa
ggcatcaaagtattagcaatgaggcctcaacagaggtgcctttaaaagaagagatatggaataaaataagtgccttttt
ctcttcagaacatcaggttgaagcacaaaactgcatcgcttatctttgtcatccacctgaaaccgcctcgccagaagag
atcaaaagcaagtttgaatgtttaaggatgttagctttcccggcgtatgcggataatattcagtatagtagaggagggg
cagaccaatactgtattttgagtgaaaatagtcaggaaattctgtctatagtttttaatacagagggctataccgttga
gggagggggaaagtcagtcacctatacccgtgtgacagaaagcgagcaggcgagtagcgcttccggctccaaagatgct
gtgaattatgagttaatctggtctgagtgggtaaaagaggcgccagcgaaagaggcagcaaatcgtgaagaagccgtac
aacggatgcgtgactgcctgaaaaataataagacggaacttcgtctgaaaatattaggacttaccggggtctcaggccg
cttcgagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatggg
cacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactg
gaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatca
tatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgac
gggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatt
ttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaa
acaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattat
caacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcga
aagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatga
actatacaaataataatactag