BBa_K552002 1 BBa_K552002 CrtYB Gene-Codon Optimized for Yeast 2011-09-26T11:00:00Z 2015-05-08T01:12:40Z The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm This DNA sequence codes for the binary enzyme CrtYB. CrtY (Lycopene Beta-cyclase) converts lycopene into beta-carotene and serves as the last step in beta-carotene biosynthesis in Saccharomyces cerevisiae. CrtB is acts further upstream of CrtY in the pathway and is called Phytoene Synthase. CrtB converts geranylgeranyl diphosphate to phytoene. false false _720_ 0 8813 9 Not in stock true While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence. false Jonathan Herrmann annotation2145068 1 ADH1 Terminator range2145068 1 2403 2531 annotation2145065 1 Start Codon range2145065 1 381 383 annotation2145064 1 PGK Promoter range2145064 1 1 380 annotation2145067 1 Stop Codon range2145067 1 2400 2402 annotation2145066 1 CrtYB range2145066 1 383 2402 BBa_K552002_sequence 1 gactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtggccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagagatcccgactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagtttttcaagttcttagatgctttctttttctcttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaatgactgctttggcttattatcaaattcatttgatttatactttgccaattttgggtttgttgggtttgttgacttctccaattttgactaaattcgatatttataaaatttctattttggttttcattgctttctctgctactactccatgggattcttggattattagaaatggtgcttggacttatccatctgctgaatctggtcaaggtgttttcggtactttcttggatgttccatatgaagaatatgctttcttcgttattcaaactgttattactggtttggtttatgttttggctactagacatttgttgccatctttggctttgccaaaaactagatcttctgctttgtctttggctttgaaagctttgattccattgccaattatttatttgttcactgctcatccatctccatctccagatccattggttactgatcattatttctatatgagagctttgtctttgttgattactccaccaactatgttgttggctgctttgtctggtgaatatgctttcgattggaaatctggtagagctaaatctactattgctgctattatgattccaactgtttatttgatttgggttgattatgttgctgttggtcaagattcttggtctattaatgatgaaaaaattgttggttggagattgggtggtgttttgccaattgaagaagctatgttcttcttgttgactaatttgatgattgttttgggtttgtctgcttgtgatcatactcaagctttgtatttgttgcatggtagaactatttatggtaataaaaaaatgccatcttctttcccattgattactccaccagttttgtctttgttcttctcttctagaccatattcttctcaaccaaaaagagatttggaattggctgttaaattgttggaagaaaaatctagatctttcttcgttgcttctgctggtttcccatctgaagttagagaaagattggttggtttgtatgctttctgtagagttactgatgatttgattgattctccagaagtttcttctaatccacatgctactattgatatggtttctgatttcttgactttgttgttcggtccaccattgcatccatctcaaccagataaaattttgtcttctccattgttgccaccatctcatccatctagaccaactggtatgtatccattgccaccaccaccatctttgtctccagctgaattggttcaattcttgactgaaagagttccagttcaatatcatttcgctttcagattgttggctaaattgcaaggtttgattccaagatatccattggatgaattgttgagaggttatactactgatttgattttcccattgtctactgaagctgttcaagctagaaaaactccaattgaaactactgctgatttgttggattatggtttgtgtgttgctggttctgttgctgaattgttggtttatgtttcttgggcttctgctccatctcaagttccagctactattgaagaaagagaagctgttttggttgcttctagagaaatgggtactgctttgcaattggttaatattgctagagatattaaaggtgatgctactgaaggtagattctatttgccattgtctttcttcggtttgagagatgaatctaaattggctattccaactgattggactgaaccaagaccacaagatttcgataaattgttgtctttgtctccatcttctactttgccatcttctaatgcttctgaatctttcagattcgaatggaaaacttattctttgccattggttgcttatgctgaagatttggctaaacattcttataaaggtattgatagattgccaactgaagttcaagctggtatgagagctgcttgtgcttcttatttgttgattggtagagaaattaaagttgtttggaaaggtgatgttggtgaaagaagaactgttgctggttggagaagagttagaaaagttttgtctgttgttatgtctggttgggaaggtcaataagcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z