BBa_K552002
1
BBa_K552002
CrtYB Gene-Codon Optimized for Yeast
2011-09-26T11:00:00Z
2015-05-08T01:12:40Z
The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
This DNA sequence codes for the binary enzyme CrtYB. CrtY (Lycopene Beta-cyclase) converts lycopene into beta-carotene and serves as the last step in beta-carotene biosynthesis in Saccharomyces cerevisiae. CrtB is acts further upstream of CrtY in the pathway and is called Phytoene Synthase. CrtB converts geranylgeranyl diphosphate to phytoene.
false
false
_720_
0
8813
9
Not in stock
true
While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
false
Jonathan Herrmann
annotation2145068
1
ADH1 Terminator
range2145068
1
2403
2531
annotation2145065
1
Start Codon
range2145065
1
381
383
annotation2145064
1
PGK Promoter
range2145064
1
1
380
annotation2145067
1
Stop Codon
range2145067
1
2400
2402
annotation2145066
1
CrtYB
range2145066
1
383
2402
BBa_K552002_sequence
1
gactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtggccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagagatcccgactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagtttttcaagttcttagatgctttctttttctcttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaatgactgctttggcttattatcaaattcatttgatttatactttgccaattttgggtttgttgggtttgttgacttctccaattttgactaaattcgatatttataaaatttctattttggttttcattgctttctctgctactactccatgggattcttggattattagaaatggtgcttggacttatccatctgctgaatctggtcaaggtgttttcggtactttcttggatgttccatatgaagaatatgctttcttcgttattcaaactgttattactggtttggtttatgttttggctactagacatttgttgccatctttggctttgccaaaaactagatcttctgctttgtctttggctttgaaagctttgattccattgccaattatttatttgttcactgctcatccatctccatctccagatccattggttactgatcattatttctatatgagagctttgtctttgttgattactccaccaactatgttgttggctgctttgtctggtgaatatgctttcgattggaaatctggtagagctaaatctactattgctgctattatgattccaactgtttatttgatttgggttgattatgttgctgttggtcaagattcttggtctattaatgatgaaaaaattgttggttggagattgggtggtgttttgccaattgaagaagctatgttcttcttgttgactaatttgatgattgttttgggtttgtctgcttgtgatcatactcaagctttgtatttgttgcatggtagaactatttatggtaataaaaaaatgccatcttctttcccattgattactccaccagttttgtctttgttcttctcttctagaccatattcttctcaaccaaaaagagatttggaattggctgttaaattgttggaagaaaaatctagatctttcttcgttgcttctgctggtttcccatctgaagttagagaaagattggttggtttgtatgctttctgtagagttactgatgatttgattgattctccagaagtttcttctaatccacatgctactattgatatggtttctgatttcttgactttgttgttcggtccaccattgcatccatctcaaccagataaaattttgtcttctccattgttgccaccatctcatccatctagaccaactggtatgtatccattgccaccaccaccatctttgtctccagctgaattggttcaattcttgactgaaagagttccagttcaatatcatttcgctttcagattgttggctaaattgcaaggtttgattccaagatatccattggatgaattgttgagaggttatactactgatttgattttcccattgtctactgaagctgttcaagctagaaaaactccaattgaaactactgctgatttgttggattatggtttgtgtgttgctggttctgttgctgaattgttggtttatgtttcttgggcttctgctccatctcaagttccagctactattgaagaaagagaagctgttttggttgcttctagagaaatgggtactgctttgcaattggttaatattgctagagatattaaaggtgatgctactgaaggtagattctatttgccattgtctttcttcggtttgagagatgaatctaaattggctattccaactgattggactgaaccaagaccacaagatttcgataaattgttgtctttgtctccatcttctactttgccatcttctaatgcttctgaatctttcagattcgaatggaaaacttattctttgccattggttgcttatgctgaagatttggctaaacattcttataaaggtattgatagattgccaactgaagttcaagctggtatgagagctgcttgtgcttcttatttgttgattggtagagaaattaaagttgtttggaaaggtgatgttggtgaaagaagaactgttgctggttggagaagagttagaaaagttttgtctgttgttatgtctggttgggaaggtcaataagcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z