BBa_K552003 1 BBa_K552003 CrtI Gene-Codon Optimized for Yeast 2011-09-27T11:00:00Z 2015-05-08T01:12:40Z The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm This DNA sequence codes for the enzyme CrtI, also known as phytoene desaturase. CrtI converts pytoene to lycopene which is an important step in the carotenoid biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator. false false _720_ 0 8813 9 Not in stock true While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence. false Jonathan Herrmann annotation2145134 1 ADH1 Terminator range2145134 1 2130 2258 annotation2145133 1 Stop Codon range2145133 1 2127 2129 annotation2145132 1 CrtI range2145132 1 381 2129 annotation2145131 1 Start Codon range2145131 1 381 383 annotation2145122 1 PGK Promoter range2145122 1 1 380 BBa_K552003_sequence 1 gactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtggccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagagatcccgactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagtttttcaagttcttagatgctttctttttctcttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaatgggtaaagaacaagatcaagataaaccaactgctattattgttggttgtggtattggtggtattgctactgctgctagattggctaaagaaggtttccaagttactgttttcgaaaaaaatgattattctggtggtagatgttctttgattgaaagagatggttatagattcgatcaaggtccatctttgttgttgttgccagatttgttcaaacaaactttcgaagatttgggtgaaaaaatggaagattgggttgatttgattaaatgtgaaccaaattatgtttgtcatttccatgatgaagaaactttcactttctctactgatatggctttgttgaaaagagaagttgaaagattcgaaggtaaagatggtttcgatagattcttgtctttcattcaagaagctcatagacattatgaattggctgttgttcatgttttgcaaaaaaatttcccaggtttcgctgctttcttgagattgcaattcattggtcaaattttggctttgcatccattcgaatctatttggactagagtttgtagatatttcaaaactgatagattgagaagagttttctctttcgctgttatgtatatgggtcaatctccatattctgctccaggtacttattctttgttgcaatatactgaattgactgaaggtatttggtatccaagaggtggtttctggcaagttccaaatactttgttgcaaattgttaaaagaaataatccatctgctaaattcaatttcaatgctccagtttctcaagttttgttgtctccagctaaagatagagctactggtgttagattggaatctggtgaagaacatcatgctgatgttgttattgttaatgctgatttggtttatgcttctgaacatttgattccagatgatgctagaaataaaattggtcaattgggtgaagttaaaagatcttggtgggctgatttggttggtggtaaaaaattgaaaggttcttgttcttctttgtctttctattggtctatggatagaattgttgatggtttgggtggtcataatattttcttggctgaagatttcaaaggttctttcgatactattttcgaagaattgggtttgccagctgatccatctttctatgttaatgttccatctagaattgatccatctgctgctccagaaggtaaagatgctattgttattttggttccatgtggtcatattgatgcttctaatccacaagattataataaattggttgctagagctagaaaattcgttattcaaactttgtctgctaaattgggtttgccagatttcgaaaaaatgattgttgctgaaaaagttcatgatgctccatcttgggaaaaagaattcaatttgaaagatggttctattttgggtttggctcataatttcatgcaagttttgggtttcagaccatctactagacatccaaaatatgataaattgttcttcgttggtgcttctactcatccaggtactggtgttccaattgttttggctggtgctaaattgactgctaatcaagttttggaatctttcgatagatctccagctccagatccaaatatgtctttgtctgttccatatggtaaaccattgaaatctaatggtactggtattgattctcaagttcaattgaaattcatggatttggaaagatgggtttatttgttggttttgttgattggtgctgttattgctagatctgttggtgttttggctttctaagcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z