BBa_K552004 1 BBa_K552004 CCD1 Gene-Codon Optimized for Yeast 2011-09-27T11:00:00Z 2015-05-08T01:12:40Z The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm This DNA sequence codes for the enzyme CCD1, also known as carotenoid cleavage dioxygenase 1. CCD1 cleaves beta-caroten to form two molecules of beta-ionone. This enzyme completes the beta-ionone biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator. false false _720_ 0 8813 9 Not in stock false While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence. false Jonathan Herrmann annotation2145139 1 Start Codon range2145139 1 381 383 annotation2145144 1 ADH1 Terminator range2145144 1 2012 2141 annotation2145143 1 Stop Codon range2145143 1 2010 2012 annotation2145142 1 CCD1 range2145142 1 381 2012 annotation2145140 1 PGK Promoter range2145140 1 1 380 BBa_K552004_sequence 1 gactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtggccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagagatcccgactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagtttttcaagttcttagatgctttctttttctcttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaatgggtatgcaaggtgaagatgctcaaagaactggtaatattgttgctgttaaaccaaaaccatctcaaggtttgacttctaaagctattgattggttggaatggttgttcgttaaaatgatgcatgattctaaacaaccattgcattatttgtctggtaatttcgctccagttgatgaaactccaccattgaaagatttgccagttactggtcatttgccagaatgtttgaatggtgaattcgttagagttggtccaaatccaaaattcgcttctattgctggttatcattggttcgatggtgatggtatgattcatggtatgagaattaaagatggtaaagctacttatgtttctagatatgttcaaacttctagattgaaacaagaagaattcttcggtagagctatgttcatgaaaattggtgatttgaaaggtatgttcggtttgttgatggttaatatgcaaatgttgagagctaaattgaaagttttggatatttcttatggtattggtactgctaatactgctttggtttatcatcatggtaaattgttggctttgtctgaagctgataaaccatatgctattaaagttttggaagatggtgatttgcaaactattggtttgttggattatgataaaagattggctcattctttcactgctcatccaaaagttgatccattcactggtgaaatgttcactttcggttattctcatactccaccatatgttacttatagagttatttctaaagatggtgctatgaatgatccagttccaattactgtttctggtccaattatgatgcatgatttcgctattactgaaaattatgctattttcatggatttgccattgtatttcaaaccaaaagaaatggttaaagataaaaaattcattttctctttcgatgctactcaaaaagctagattcggtattttgccaagatatgctaaaaatgaattgttgattaaatggttcgaattgccaaattgtttcattttccataatgctaatgcttgggaagaaggtgatgaagttgttttgattacttgtagattggaaaatccagatttggatatggttaattctactgttaaagaaagattggataatttcaaaaatgaattgtatgaaatgagattcaatttgcaaaatggtttggcttctcaaaaaaaattgtctgtttctgctgttgatttcccaagagttaatgaatcttatactactagaaaacaaagatatgtttatggtactactttggataaaattgctaaagttactggtattattaaattcgatttgcatgctgaaccagaaactggtaaagaaaaattggaattgggtggtaatgttaaaggtattttcgatttgggtccaggtagattcggttctgaagctgttttcgttccaagacatccaggtattacttctgaagaagatgatggttatttgattttcttcgttcatgatgaaaatactggtaaatctgctgttaatgttattgatgctaaaactatgtctccagatccagttgctgttgttgaattgccaaaaagagttccatatggtttccatgctttcttcgttactgaagatcaattgcaagaacaagctaaagtttaagcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z