BBa_C0078
1
lasI
autoinducer synthetase for PAI from Pseudomonas aeruginosa
2004-01-27T12:00:00Z
2015-08-31T04:07:24Z
www.ncbi.nlm.nih.gov
Released HQ 2013
coding region for lasI protein, which produces the chemical signal AI-1
false
false
_1_
0
24
7
In stock
false
true
Chris Walsh (Fighting Darwins)
annotation2214003
1
Help:Barcodes
range2214003
1
643
667
annotation305970
1
LasI
range305970
1
1
603
annotation306608
1
LVA
range306608
1
604
636
BBa_K081016
1
BBa_K081016
lasI protein generator - PoPS->lasI
2008-10-18T11:00:00Z
2015-05-08T01:08:35Z
RBS:
lasI:
T:
Released HQ 2013
PoPS in -> lasI out.
<br>
Strong RBS (efficiency=0.6).
<br>
Artificial 39 bp terminator (efficiency=0.99).
false
false
_227_
0
2583
9
In stock
true
We used BioBrick Standard Assembly.
true
Lorenzo Pasotti, Paolo Magni
component2246162
1
BBa_C0078
component2246157
1
BBa_B0030
component2246167
1
BBa_B1006
annotation2246157
1
BBa_B0030
range2246157
1
1
15
annotation2246167
1
BBa_B1006
range2246167
1
697
735
annotation2246162
1
BBa_C0078
range2246162
1
22
688
BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898429
1
modified thr terminator
range1898429
1
10
31
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
BBa_K553004
1
BBa_K553004
OC12 HLA in response to OC8 HLA
2011-09-16T11:00:00Z
2015-05-08T01:12:40Z
wefrfewq
erf22f
false
false
_721_
0
8730
9
It's complicated
false
ewrfref
false
Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto
component2250658
1
BBa_K081016
component2250645
1
BBa_K553002
annotation2250645
1
BBa_K553002
range2250645
1
1
151
annotation2250658
1
BBa_K081016
range2250658
1
160
894
BBa_K553002
1
BBa_K553002
PTraI - TraR-OC8 HLA complex regulated promoter
2011-09-16T11:00:00Z
2015-05-08T01:12:40Z
http://www.ncbi.nlm.nih.gov/nucleotide/8572673?report=genbank&log$=nuclalign&blast_rank=1&RID=795BNUZN014
PTraI promoter was obtained by PCR from Agrobacterium Tumefaciens gDNA.
This part is positively regulated in presence of the TraR trans-activator and oc8 HLA.
false
false
_721_
0
8730
9
It's complicated
false
the pcr amplification worked well, few mutation observed after sequencing that are present in our strain
false
Giulio Bernardinelli
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K553004_sequence
1
aagtaacgcgaagtgcagatttgcacatgaaatcaacgctgcacgagatcgattgcacgcgcaaatcgctttttgaagactgactcagtagaatcactacgtgcagatctgcacatagccacaccctgaatgagatgttttctctccgctatactagagattaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_C0078_sequence
1
atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K081016_sequence
1
attaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K553002_sequence
1
aagtaacgcgaagtgcagatttgcacatgaaatcaacgctgcacgagatcgattgcacgcgcaaatcgctttttgaagactgactcagtagaatcactacgtgcagatctgcacatagccacaccctgaatgagatgttttctctccgcta
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z