LOCUS BBa_K553006 1989 bp DNA linear 05-Nov-2024 DEFINITION C. firmi beta-glucosidase PlasB regulated ACCESSION BBa_K553006 VERSION BBa_K553006.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 166..168 /label=SacI RBS 169..175 /label=rbs RBS 166..175 /label=BBa_J15001 misc_feature 182..1852 /label=BBa_K392008 stem_loop 1872..1915 /label=stem_loop misc_feature 1861..1940 /label=BBa_B0010 terminator 1861..1940 /label=BBa_B0010 stem_loop 1956..1975 /label=T7 TE misc_feature 1982..1982 /label=stop polyA_site 1976..1989 /label=polya misc_feature 1949..1989 /label=BBa_B0012 terminator 1949..1989 /label=BBa_B0012 terminator 1861..1989 /label=BBa_B0015 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagctcaa ggaggtacta 181 gatgggcgac cggttccagc aggccggtcg cccacgccgc cgcggcccgg cgagggccgt 241 taaccgtacc ggtcaagaag acgcgtcgac ggggtcgagg gagcggtccc acgcgtgtat 301 cgtatcgttt cgacaccgcc acccggccac cgggcacgca ccggggacgc agcagtcccc 361 gccccggcca ccccctgtca ccgaaacccg aaggaccctc atgaccacca cgcgcccctc 421 gggccgccag ttctccgacg acttcctgtg gggctcggcc acggcctcct accagatcga 481 gggcgcgcac gacgagggcg gtcgcggccc gtcgatctgg gacacgttct cccgcacccc 541 gggcaaggtc ctcaacggcg acaccggcga cgtcgcggtc gaccactacc accgcgtccc 601 cgaggacgtc gagatcatga agagcctcgg cctccaggcc taccggttct cgatcgcctg 661 gccccgcatc cagccgaccg gctcgggcga gttcaaccag gccgggctcg acttctactc 721 cgacctcgtc gaccggctca tcgccgccgg catcaagccc gtcgcgacgc tgtaccactg 781 ggacctcccg cagcccctgg aggacgaggg cggctgggcg aaccgcgcga cggcgtaccg 841 gttcgtcgag tacgcgcgca agctcgccga ggtcctcggc aagcgcgtcg acctgtggac 901 cacgctcaac gagccgtggt gctccgcgtt cctcggctac gcctcaggcg tgcacgcccc 961 ggcgtcaccg acccggtcaa ggccctgcgc gccgtccacc acctcaacct cgcgcacggc 1021 ctcgcgggcc gcgcgatccg cgaggtcctc ggcgagaacg cgcccgtctc gatcacgtga 1081 acctgcacgt gacgcgcgcc gccgacgact ccgccgactc cgtcgaggcc aagcgccgca 1141 tcgacaccat cgcgaacgag gtcttcctcg gcccgctgct cgacggcgag taccccaagg 1201 aggtcttcgc ggacaccgcg caccacaccg actggtcctt cgtcgagccg ggcgacctcg 1261 agctcatccg catcccgctc accgtgctcg gcgtcaacta ctactcgacc ggccgcgtga 1321 agaagggcag cgcgcgggcg ccgacccggg caagccgggc cccgacggcc accgcgcctc 1381 cgagcactcc tcgtgggtcg gcgccgacga ggtcgagtgg ctcccgcagc ccggcccgca 1441 caccgcgatg ggctggaaca tcgagcccga cggcctcgtc gacctgctgc tcgagctgcg 1501 cgaccgttac cccagccagc ccctggcgat cacggagaac ggtgccgcgt tctacgacac 1561 ggtcagcgag gacggccgcg tgcacgaccc cgagcgcgtc ggctacctgc acgaccacgt 1621 cgacgccgtc ggcgaggcca tcgacaaggg cgccgacgtg cgcgcctact tcgtgtggtc 1681 gctgctcgac aacttcgagt ggcgctacgg ctacgaccgc cggttcggca tcgtgcgcgt 1741 cgactacgac acgcacgagc gcatcgtgaa ggactcgggc ctctggtacc gcgagctcgt 1801 gcgcacccgc accatcgctc cggccgagga cgccgcgacc taccagccct gatactagag 1861 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1921 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1981 gcgtttata //