BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K553001
1
BBa_K553001
A. tumefaciens TraI - OC8 HLA synthase
2011-09-16T11:00:00Z
2015-05-08T01:12:40Z
http://www.ncbi.nlm.nih.gov/nucleotide/81176483?report=genbank&log$=nuclalign&blast_rank=1&RID=79664K7A015
This part was obtained by PCR from Agrobacterium Tumefaciens gDNA.
This trans-activator binds oc8 HLA and then positively regulates PTraI promoter
false
false
_721_
0
8730
9
It's complicated
false
this coding sequence become a standard Biobrick via per without any particular problems
false
Giulio Bernardinelli
annotation2130091
1
start
range2130091
1
1
3
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0079
1
LasR+PAI
Promoter (LasR & PAI regulated)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
"Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
Released HQ 2013
Binding region for LasR protein (positive regulation)
false
true
_1_
0
24
7
In stock
false
true
Alvin Carter Powers (Fighting Darwins)
annotation318496
1
-10
range318496
1
140
145
annotation300992
1
OP1
range300992
1
106
125
annotation300985
1
OP2
range300985
1
46
63
annotation318495
1
-35
range318495
1
117
122
BBa_K553008
1
BBa_K553008
OC8 HLA synthase induced by OC12 HLA
2011-09-16T11:00:00Z
2015-05-08T01:12:40Z
nol
jubbju
false
false
_721_
0
8730
9
It's complicated
true
nono
false
Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto
component2130236
1
BBa_B0034
component2130238
1
BBa_K553001
component2130234
1
BBa_R0079
component2130245
1
BBa_B0015
annotation2130234
1
BBa_R0079
range2130234
1
1
157
annotation2130238
1
BBa_K553001
range2130238
1
184
822
annotation2130236
1
BBa_B0034
range2130236
1
166
177
annotation2130245
1
BBa_B0015
range2130245
1
831
959
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K553008_sequence
1
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatactagatgctgattctgaccgtctcgcccgaccaataccaacaccagaacagttacctcaagcaaatgcaccggcttcgagccgaagtgtttggaaaccgtctgaaatgggatgtcgcaatagaggacggcggcgaacgggatcaatacgacgagctcagcccgacctacattctcgcgacgttcggtgggcagagggtggtcggctgcgcacggcttcttgcgccatcaggaccgaccatgctggagcggacgtttccgcaattgctggcaaccggctccctcagcgcaaccacggcaatgatcgaaacgtcacgcttctgcgtcgacacgacattgccgaccgggagggcagggaggcaactgcatctcgcgacgctcaccatgttcgccggcatcatcgaatggtcgatggcaaacggctacgacgaaattgtcacggcaaccgatcttcgcttcgaacgcatcttgaagcgcgccggttggccgatgacgcgactgggcgaacccgtcgcgattggcaacaccgtcgccgtcgccggacatttgcccgccgaccgaaaaagcttcgaacgggtctgcccgccgggataccgttcgattatcgctgacgataacggccgcccgttgaggagtgcggcgtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K553001_sequence
1
atgctgattctgaccgtctcgcccgaccaataccaacaccagaacagttacctcaagcaaatgcaccggcttcgagccgaagtgtttggaaaccgtctgaaatgggatgtcgcaatagaggacggcggcgaacgggatcaatacgacgagctcagcccgacctacattctcgcgacgttcggtgggcagagggtggtcggctgcgcacggcttcttgcgccatcaggaccgaccatgctggagcggacgtttccgcaattgctggcaaccggctccctcagcgcaaccacggcaatgatcgaaacgtcacgcttctgcgtcgacacgacattgccgaccgggagggcagggaggcaactgcatctcgcgacgctcaccatgttcgccggcatcatcgaatggtcgatggcaaacggctacgacgaaattgtcacggcaaccgatcttcgcttcgaacgcatcttgaagcgcgccggttggccgatgacgcgactgggcgaacccgtcgcgattggcaacaccgtcgccgtcgccggacatttgcccgccgaccgaaaaagcttcgaacgggtctgcccgccgggataccgttcgattatcgctgacgataacggccgcccgttgaggagtgcggcgtga
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0079_sequence
1
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z