LOCUS       BBa_K553009             1867 bp    DNA     linear       29-Jun-2024
DEFINITION  constitutive production of beta-glucosidase
ACCESSION   BBa_K553009
VERSION     BBa_K553009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    44..46
                     /label=SacI
     RBS             47..53
                     /label=rbs
     RBS             44..53
                     /label=BBa_J15001
     misc_feature    60..1730
                     /label=BBa_K392008
     stem_loop       1750..1793
                     /label=stem_loop
     misc_feature    1739..1818
                     /label=BBa_B0010
     terminator      1739..1818
                     /label=BBa_B0010
     stem_loop       1834..1853
                     /label=T7 TE
     misc_feature    1860..1860
                     /label=stop
     polyA_site      1854..1867
                     /label=polya
     misc_feature    1827..1867
                     /label=BBa_B0012
     terminator      1827..1867
                     /label=BBa_B0012
     terminator      1739..1867
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagctcaagg aggtactaga
       61 tgggcgaccg gttccagcag gccggtcgcc cacgccgccg cggcccggcg agggccgtta
      121 accgtaccgg tcaagaagac gcgtcgacgg ggtcgaggga gcggtcccac gcgtgtatcg
      181 tatcgtttcg acaccgccac ccggccaccg ggcacgcacc ggggacgcag cagtccccgc
      241 cccggccacc ccctgtcacc gaaacccgaa ggaccctcat gaccaccacg cgcccctcgg
      301 gccgccagtt ctccgacgac ttcctgtggg gctcggccac ggcctcctac cagatcgagg
      361 gcgcgcacga cgagggcggt cgcggcccgt cgatctggga cacgttctcc cgcaccccgg
      421 gcaaggtcct caacggcgac accggcgacg tcgcggtcga ccactaccac cgcgtccccg
      481 aggacgtcga gatcatgaag agcctcggcc tccaggccta ccggttctcg atcgcctggc
      541 cccgcatcca gccgaccggc tcgggcgagt tcaaccaggc cgggctcgac ttctactccg
      601 acctcgtcga ccggctcatc gccgccggca tcaagcccgt cgcgacgctg taccactggg
      661 acctcccgca gcccctggag gacgagggcg gctgggcgaa ccgcgcgacg gcgtaccggt
      721 tcgtcgagta cgcgcgcaag ctcgccgagg tcctcggcaa gcgcgtcgac ctgtggacca
      781 cgctcaacga gccgtggtgc tccgcgttcc tcggctacgc ctcaggcgtg cacgccccgg
      841 cgtcaccgac ccggtcaagg ccctgcgcgc cgtccaccac ctcaacctcg cgcacggcct
      901 cgcgggccgc gcgatccgcg aggtcctcgg cgagaacgcg cccgtctcga tcacgtgaac
      961 ctgcacgtga cgcgcgccgc cgacgactcc gccgactccg tcgaggccaa gcgccgcatc
     1021 gacaccatcg cgaacgaggt cttcctcggc ccgctgctcg acggcgagta ccccaaggag
     1081 gtcttcgcgg acaccgcgca ccacaccgac tggtccttcg tcgagccggg cgacctcgag
     1141 ctcatccgca tcccgctcac cgtgctcggc gtcaactact actcgaccgg ccgcgtgaag
     1201 aagggcagcg cgcgggcgcc gacccgggca agccgggccc cgacggccac cgcgcctccg
     1261 agcactcctc gtgggtcggc gccgacgagg tcgagtggct cccgcagccc ggcccgcaca
     1321 ccgcgatggg ctggaacatc gagcccgacg gcctcgtcga cctgctgctc gagctgcgcg
     1381 accgttaccc cagccagccc ctggcgatca cggagaacgg tgccgcgttc tacgacacgg
     1441 tcagcgagga cggccgcgtg cacgaccccg agcgcgtcgg ctacctgcac gaccacgtcg
     1501 acgccgtcgg cgaggccatc gacaagggcg ccgacgtgcg cgcctacttc gtgtggtcgc
     1561 tgctcgacaa cttcgagtgg cgctacggct acgaccgccg gttcggcatc gtgcgcgtcg
     1621 actacgacac gcacgagcgc atcgtgaagg actcgggcct ctggtaccgc gagctcgtgc
     1681 gcacccgcac catcgctccg gccgaggacg ccgcgaccta ccagccctga tactagagcc
     1741 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1801 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1861 gtttata
//