##gff-version 3
##sequence-region BBa_K553010 1 778
BBa_K553010	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K553010	.	engineered_region	44	778	.	+	0	ID=BBa_K081016;Name=BBa_K081016
BBa_K553010	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081016
BBa_K553010	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K553010	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K553010	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K553010	.	CDS	65	731	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K081016
BBa_K553010	.	tag	668	700	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K553010	.	CDS	65	667	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K553010	.	engineered_tag	707	731	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K553010	.	terminator	740	778	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081016
BBa_K553010	.	polyA_site	771	778	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K553010	.	stem_loop	749	770	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K553010	.	engineered_region	740	778	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K553010	.	polyA_site	740	748	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
>BBa_K553010
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgatcgttcagatc
ggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaag
gttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccagga
agacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttc
ccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtc
agaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacat
ccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggt
ccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacg
gtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctg
atagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt