LOCUS BBa_K554013 5325 bp DNA linear 25-Nov-2024 DEFINITION SoxS HlyB HlyD TolC device ACCESSION BBa_K554013 VERSION BBa_K554013.1 FEATURES Location/Qualifiers promoter 1..62 /label=SoxS promoter 1..62 /label=BBa_K554000 RBS 71..85 /label=RBS CDS 86..2206 /label=HlyB misc_feature 2207..2212 /label=stop CDS 71..2212 /label=BBa_K554007 RBS 2221..2235 /label=RBS CDS 2236..3669 /label=HlyD misc_feature 3670..3675 /label=stop CDS 2221..3675 /label=BBa_K554008 RBS 3684..3698 /label=RBS CDS 3699..5216 /label=TolC misc_feature 5217..5222 /label=stop CDS 3684..5222 /label=BBa_K554009 stem_loop 5238..5257 /label=T7 TE misc_feature 5264..5264 /label=stop polyA_site 5258..5271 /label=polya misc_feature 5231..5271 /label=BBa_B0012 terminator 5231..5271 /label=BBa_B0012 stem_loop 5292..5314 /label=stem_loop misc_feature 5280..5325 /label=BBa_B0011 terminator 5280..5325 /label=BBa_B0011 terminator 5231..5325 /label=BBa_B0014 ORIGIN 1 aatcgcttta cctcaagtta acttgaggaa ttatactccc caacagatga attaacgaac 61 tgtactagag attaaagagg agaaaatgga ttcctgtcac aagattgatt acggcctgta 121 tgcactggaa atcctggcac aatatcataa cgtctccgtc aacccggagg aaattaaaca 181 tcgcttcgat actgacggca ctggcctggg tctgaccagc tggctgctgg cagcgaagtc 241 cctggagctg aaggtaaagc aagtgaaaaa gacgattgat cgtctgaact tcatcagcct 301 gccggcactg gtttggcgtg aagatggccg tcacttcatc ctgacgaaag tgtccaaaga 361 agcgaaccgc tacctgatct ttgatctgga gcagcgcaac ccgcgcgtac tggaacagag 421 cgagttcgag gctctgtatc agggccacat cattctgatt gcatctcgtt cttctgttgc 481 aggtaaactg gcgaaattcg acttcacctg gttcattccg gctattatca agtaccgtcg 541 tattttcatc gaaaccctgg ttgtatctgt tttcctgcaa ctgttcgctc tgattactcc 601 gctgttcttt caggttgtca tggacaaagt tctggttcac cgtggctttt ccaccctgaa 661 cgtcattact gttgcgctga gcgttgttgt tgtgttcgaa atcattctgt ccggtctgcg 721 tacctacatc ttcgcgcact ctacctcccg catcgacgtt gaactgggtg cgaagctgtt 781 ccgtcacctg ctggcactgc ctatcagcta cttcgaatct cgtcgtgtgg gcgatactgt 841 ggcccgtgtt cgtgaactgg atcagatccg caactttctg accggccagg ccctgaccag 901 cgttctggat ctgctgttca gctttatctt cttcgcagta atgtggtact acagcccgaa 961 gctgactctg gtgattctgt tctctctgcc gtgttacgca gcttggtccg tcttcatctc 1021 tccaatcctg cgtcgccgtc tggacgacaa atttagccgt aatgcggata atcagtcctt 1081 cctggtcgaa tctgtgactg ctatcaacac tatcaaagcc atggcggttt ctccacagat 1141 gactaacatc tgggataagc agctggcagg ttacgttgct gcgggcttca aagtgaccgt 1201 tctggcaacc attggtcaac agggcattca gctgattcag aaaaccgtga tgatcattaa 1261 cctgtggctg ggtgcgcacc tggtcatcag cggtgacctg tccattggcc agctgatcgc 1321 tttcaacatg ctggcgggtc aaattgttgc cccagtaatc cgtctggccc agatctggca 1381 ggacttccag caggtcggta tttctgtgac ccgcctgggt gacgtcctga actctccgac 1441 cgaaagctac cacggtaaac tggctctgcc ggagatcaac ggcgatatta ctttccgtaa 1501 catccgtttt cgttacaaac cggattcccc ggtaatcctg gataacatca atctgtctat 1561 caaacagggc gaagtcatcg gcatcgtggg ccgtagcggc agcggcaagt ccactctgac 1621 taaactgatt cagcgctttt acattccaga aaacggtcag gttctgattg acggtcacga 1681 cctggctctg gcggacccga actggctgcg tcgtcaggtg ggcgtagtgc tgcaagataa 1741 cgtgctgctg aaccgtagca tcatcgataa catcagcctg gctaacccgg gcatgtccgt 1801 ggaaaaagtt atctatgctg cgaaactggc aggcgcgcac gatttcatct ctgaactgcg 1861 tgaaggctac aacaccatcg taggcgaaca gggcgctggc ctgtccggcg gtcaacgtca 1921 gcgtatcgct attgcacgtg cactggtgaa caacccgaaa atcctgatct tcgacgaggc 1981 cacttctgct ctggattacg aatctgaaca tattatcatg cgtaatatgc acaagatctg 2041 caaaggtcgc accgtaatca tcatcgccca ccgtctgagc accgtaaaga acgccgaccg 2101 catcatcgtg atggaaaaag gtaagatcgt tgaacagggt aaacataaag agctgctgtc 2161 cgaaccggaa tctctgtatt cttatctgta ccagctgcaa tctgattaat aatactagag 2221 attaaagagg agaaaatgaa aacttggctg atgggcttct ctgagttcct gctgcgttat 2281 aagctggtat ggagcgaaac ttggaaaatc cgtaagcaac tggatacccc ggtacgtgaa 2341 aaagacgaaa acgaatttct gccagcacat ctggaactga ttgagacgcc ggtttctcgc 2401 cgtccacgtc tggttgctta cttcattatg ggcttcctgg ttattgctgt aatcctgtct 2461 gttctgggtc aggtggaaat cgttgcgacc gcgaatggta aactgaccct gtccggtcgt 2521 tctaaagaaa tcaaaccgat cgaaaactcc atcgttaaag agattatcgt taaagaaggt 2581 gaatccgtac gcaaaggcga tgttctgctg aaactgaccg cactgggcgc agaagcggac 2641 actctgaaaa ctcagtcctc tctgctgcaa acccgtctgg aacagacccg ttaccaaatc 2701 ctgagccgtt ctattgaact gaacaaactg ccggaactga aactgccgga tgaaccatac 2761 ttccagaacg taagcgagga ggaagttctg cgcctgactt ccctgatcaa agagcagttc 2821 tctacttggc agaaccagaa ataccaaaag gaactgaacc tggacaaaaa gcgtgcggag 2881 cgcctgacta tcctggcccg catcaaccgt tacgagaacc tgtctcgtgt tgaaaaatct 2941 cgcctggacg acttccgtag cctgctgcac aaacaggcca tcgcaaaaca cgctgtgctg 3001 gaacaggaaa ataaatacgt ggaagctgcc aacgaactgc gtgtatataa aagccagctg 3061 gagcagatcg agagcgaaat cctgtctgcg aaggaggagt accagctggt gacgcagctg 3121 ttcaaaaatg aaatcctgga caaactgcgt caaaccacgg actctatcga actgctgacc 3181 ctggaactgg agaaaaacga ggagcgccag caggccagcg tcatccgcgc tccggtatct 3241 ggtaaagttc agcaactgaa agttcacacc gaaggtggcg ttgttaccac cgcagaaacc 3301 ctgatggtta ttgtgccgga agatgacacc ctggaagtta ccgctctggt gcagaacaaa 3361 gatatcggtt tcatcaacgt cggtcagaac gcgatcatca aagtagaggc tttcccgtac 3421 actcgttacg gctacctggt gggcaaggtc aaaaacatca acctggacgc tatcgaagat 3481 cagaaactgg gcctggtgtt caacgtgatc gttagcgtag aagagaacga tctgtctacc 3541 ggtaacaaac acattccact gtcctccggc atggcggtta ctgctgaaat caaaaccggt 3601 atgcgtagcg tgatctccta tctgctgtct ccgctggaag aaagcgtcac cgaatctctg 3661 cacgaacgct aataatacta gagattaaag aggagaaaat gattgccctg aacactgcct 3721 cgccacaggg gatgcagatg aagaagctcc tgccgatcct gatcgggcta agtctttccg 3781 ggttcagctc actttcacaa gccgagaatc ttatgcaagt atatcagcag gcacggctgt 3841 cgaatccaga gcttcgtaaa tcagcggccg atcgagatgc tgcgttcgag aaaattaatg 3901 aggcccggtc accgctgtta ccacagctgg gtctcggcgc ggattataca tacagcaacg 3961 gctaccgtga tgcgaatgga ataaatagta acgcaaccag cgcaagctta caactgacac 4021 agtcgatctt cgatatgtcg aaatggcgcg cactaacgct tcaggaaaaa gcagccggga 4081 tccaagatgt aacttatcag accgaccagc aaacattgat tttgaatact gcaaccgctt 4141 actttaacgt tctgaatgcc atcgacgtgc tgagctacac ccaggcacaa aaggaagcca 4201 tttatcgtca acttgaccag actacgcagc ggtttaacgt tgggttagta gcaatcaccg 4261 atgttcaaaa tgcgcgtgcg cagtatgata ccgttctggc caatgaggtc accgcacgca 4321 acaatcttga taatgcggtt gaacagttac gtcagattac cgggaactat tatcccgagc 4381 tggcagcgct gaatgtggaa aattttaaaa ctgacaaacc tcaacctgtc aataccctgc 4441 tcaaggaagc cgagaaacgc aacctgtctc tattgcaagc tcgcctgtct caagacctgg 4501 ctcgggagca aatacgccag gcacaggacg gacatctgcc cacactggac ctgaccgcaa 4561 gtactgggat atccgacacc agttactctg gatcaaaaac tcgtggcgcc gccggaacac 4621 aatacgatga ctcaaatatg ggccagaaca aggttggcct gtccttcagt ttaccgatct 4681 accagggcgg gatggtgaat agtcaagtaa aacaggcgca gtataacttt gtcggtgcat 4741 cagagcaact ggaatcagct caccgtagcg tcgtacagac tgtccggagt agctttaaca 4801 acattaacgc gagtatctca tcaatcaatg cttacaagca agcagtggtt tctgcgcagt 4861 cttctttgga tgccatggaa gcaggctatt cagtgggtac tcgcacgatt gttgatgttc 4921 tcgacgctac cacaactctc tacaacgcga aacaggaatt agcgaatgcc cgttataact 4981 acttaattaa ccaactgaat atcaaatctg ccctaggtac tctgaatgag caagacttgc 5041 ttgcgttaaa taacgccctg tccaaacctg tctcaaccaa tcctgagaac gtagcacctc 5101 agacccctga acagaacgcc attgcggacg gttacgcccc tgacagtcct gcgcctgtgg 5161 tccagcagac gtcagcgcgc actaccactt caaacgggca taatccgttc cgcaattaat 5221 aatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga 5281 gagaatataa aaagccagat tattaatccg gcttttttat tattt //