BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K554009 1 BBa_K554009 TolC 2011-09-20T11:00:00Z 2015-05-08T01:12:40Z Synthesized sequence. Released HQ 2013 TolC is ... false false _722_ 0 7971 9 In stock true false UNICAMP EMSE Brazil team annotation2136928 1 stop range2136928 1 1534 1539 annotation2136927 1 TolC range2136927 1 16 1533 annotation2136926 1 RBS range2136926 1 1 15 BBa_K554007 1 BBa_K554007 Hemolysin B - HlyB 2011-09-20T11:00:00Z 2015-05-08T01:12:40Z Synthesized sequence. HylB is ... false false _722_ 0 7971 9 It's complicated true false UNICAMP EMSE Brazil team annotation2136911 1 HlyB range2136911 1 16 2136 annotation2136910 1 RBS range2136910 1 1 15 annotation2136912 1 stop range2136912 1 2137 2142 BBa_K554013 1 BBa_K554013 SoxS HlyB HlyD TolC device 2011-09-25T11:00:00Z 2015-05-08T01:12:40Z Synthesized sequence and Registry entries. SoxS HlyB HlyD TolC device is ... false false _722_ 0 7971 9 It's complicated true false UNICAMP EMSE Brazil team component2141740 1 BBa_K554009 component2141736 1 BBa_K554008 component2141728 1 BBa_K554000 component2141732 1 BBa_K554007 component2141747 1 BBa_B0014 annotation2141747 1 BBa_B0014 range2141747 1 5231 5325 annotation2141736 1 BBa_K554008 range2141736 1 2221 3675 annotation2141732 1 BBa_K554007 range2141732 1 71 2212 annotation2141740 1 BBa_K554009 range2141740 1 3684 5222 annotation2141728 1 BBa_K554000 range2141728 1 1 62 BBa_K554008 1 BBa_K554008 Hemolysin D - HlyD 2011-09-20T11:00:00Z 2015-05-08T01:12:40Z Synthesized sequence. HlyD is ... false false _722_ 0 7971 9 It's complicated true false UNICAMP EMSE Brazil team annotation2136919 1 stop range2136919 1 1450 1455 annotation2136918 1 HlyD range2136918 1 16 1449 annotation2136917 1 RBS range2136917 1 1 15 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K554000 1 BBa_K554000 SoxS promoter 2011-09-20T11:00:00Z 2015-05-08T01:12:40Z Synthesized sequence. Released HQ 2013 SoxS promoter false false _722_ 0 7971 9 In stock true false UNICAMP_EMSE Brazil team annotation2136873 1 SoxS range2136873 1 1 62 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K554013_sequence 1 aatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagagattaaagaggagaaaatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaataatactagagattaaagaggagaaaatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaataatactagagattaaagaggagaaaatgattgccctgaacactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaattaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K554007_sequence 1 attaaagaggagaaaatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaataa BBa_K554008_sequence 1 attaaagaggagaaaatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaataa BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K554000_sequence 1 aatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactg BBa_K554009_sequence 1 attaaagaggagaaaatgattgccctgaacactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z