LOCUS       BBa_K554014             2007 bp    DNA     linear       22-Dec-2024
DEFINITION  flhDC IL12 HlyA device
ACCESSION   BBa_K554014
VERSION     BBa_K554014.1  
FEATURES             Location/Qualifiers
     promoter        1..85
                     /label=flhDC
     promoter        1..85
                     /label=BBa_K554001
     RBS             94..108
                     /label=RBS
     CDS             109..1703
                     /label=IL12
     misc_feature    1704..1710
                     /label=stop
     CDS             94..1710
                     /label=BBa_K554005
     CDS             1719..1898
                     /label=HlyA
     misc_feature    1899..1904
                     /label=Stop
     CDS             1719..1904
                     /label=BBa_K554002
     stem_loop       1920..1939
                     /label=T7 TE
     misc_feature    1946..1946
                     /label=stop
     polyA_site      1940..1953
                     /label=polya
     misc_feature    1913..1953
                     /label=BBa_B0012
     terminator      1913..1953
                     /label=BBa_B0012
     stem_loop       1974..1996
                     /label=stem_loop
     misc_feature    1962..2007
                     /label=BBa_B0011
     terminator      1962..2007
                     /label=BBa_B0011
     terminator      1913..2007
                     /label=BBa_B0014
ORIGIN
        1 ttttttaccg gggcttcccg gcgacatcac ggggtgcggt gaaaccgcat aaaaataaag
       61 ttggttattc tgggtgggaa taatgtacta gagattaaag aggagaaaat gtgccaccag
      121 cagctggtaa tctcctggtt ctccctggta ttcctggcat ccccgctggt agcaatctgg
      181 gaactgaaaa aagacgtata cgtagtagaa ctggactggt acccggacgc accgggcgaa
      241 atggtagtac tgacgtgcga cacgccggaa gaagacggca tcacgtggac gctggaccag
      301 tcctccgaag tactgggctc cggcaaaacg ctgacgatcc aggtaaaaga atttggcgac
      361 gcaggccagt acacgtgcca caaaggcggc gaagtactgt cccactccct gctgctgctg
      421 cacaaaaaag aagacggcat ctggtccacg gacatcctga aagaccagaa agaaccgaaa
      481 aacaaaacgt tcctgcgttg cgaagcaaaa aactactccg gccgtttcac gtgctggtgg
      541 ctgacgacga tctccacgga cctgacgttc tccgtaaaat cctcccgtgg ctcctccgac
      601 ccgcagggcg taacgtgcgg cgcagcaacg ctgtccgcag aacgtgtacg tggcgacaac
      661 aaagaatacg aatactccgt agaatgccag gaagactccg catgcccggc agcagaagaa
      721 tccctgccga tcgaagtaat ggtagacgca gtacacaaac tgaaatacga aaactacacg
      781 tcctccttct tcatccgtga catcatcaaa ccggacccgc cgaaaaacct gcaactgaaa
      841 ccgctgaaaa actcccgtca ggtagaagta tcctgggaat acccggacac gtggtccacg
      901 ccgcactcct acttctccct gacgttctgc gtacaggtac agggcaaatc caaacgtgaa
      961 aaaaaagacc gtgtattcac ggacaaaacg tccgcaacgg taatctgccg taaaaacgca
     1021 tccatctccg tacgtgcaca ggaccgttac tactcctcct cctggtccga atgggcatcc
     1081 gtaccgtgct ccggcggcgg cggcggcggc tcccgtaacc tgccggtagc aacgccggac
     1141 ccgggcatgt tcccgtgcct gcaccactcc cagaacctgc tgcgtgcagt atccaacatg
     1201 ctgcaaaaag cacgtcagac gctggaattt tacccgtgca cgtccgaaga aatcgaccac
     1261 gaagacatca cgaaagacaa aacgtccacg gtagaagcat gcctgccgct ggaactgacg
     1321 aaaaacgaat cctgcctgaa ctcccgtgaa acgtccttca tcacgaacgg ctcctgcctg
     1381 gcatcccgta aaacgtcctt catgatggca ctgtgcctgt cctccatcta cgaagacctg
     1441 aaaatgtacc aggtagaatt taaaacgatg aacgcaaaac tgctgatgga cccgaaacgt
     1501 cagatcttcc tggaccagaa catgctggca gtaatcgacg aactgatgca ggcactgaac
     1561 ttcaactccg aaacggtacc gcagaaatcc tccctggaag aaccggactt ctacaaaacg
     1621 aaaatcaaac tgtgcatcct gctgcacgca ttccgtatcc gtgcagtaac gatcgaccgt
     1681 gtaatgtcct acctgaacgc atcctaataa tactagagtt agcctatgga agtcagggtg
     1741 atcttaatcc attaattaat gaaatcagca aaatcatttc agcagcaggt agcttcgatg
     1801 ttaaagagga aagaaccgca gcttctttat tgcagttgtc cggtaatgcc agtgattttt
     1861 catatggacg gaactcaata accctgacca catcagcata ataatactag agtcacactg
     1921 gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag
     1981 attattaatc cggctttttt attattt
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