LOCUS       BBa_K557003              996 bp    DNA     linear       13-Jun-2024
DEFINITION  luxpR+cI: for modulating the ratio of Toggle switch
ACCESSION   BBa_K557003
VERSION     BBa_K557003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..78
                     /label=RBS-1\Strong
     RBS             71..75
                     /label=RBS
     misc_feature    64..78
                     /label=BBa_B0030
     RBS             64..78
                     /label=BBa_B0030
     CDS             88..795
                     /label=cI lambda
     misc_feature    796..828
                     /label=LVA
     misc_feature    835..859
                     /label=Help:Barcodes
     CDS             85..859
                     /label=BBa_C0051
     stem_loop       879..922
                     /label=stem_loop
     misc_feature    868..947
                     /label=BBa_B0010
     terminator      868..947
                     /label=BBa_B0010
     stem_loop       963..982
                     /label=T7 TE
     misc_feature    989..989
                     /label=stop
     polyA_site      983..996
                     /label=polya
     misc_feature    956..996
                     /label=BBa_B0012
     terminator      956..996
                     /label=BBa_B0012
     terminator      868..996
                     /label=BBa_B0015
     misc_feature    85..996
                     /label=BBa_K228236
     misc_feature    64..996
                     /label=BBa_K228237
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagattaaag aggagaaata ctagatgagc acaaaaaaga aaccattaac acaagagcag
      121 cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta
      181 tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt
      241 aatggcatca atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt
      301 agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt
      361 agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca
      421 gggatgttct cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc
      481 acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc
      541 gcaccaacag gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag
      601 caggctgttg agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc
      661 aagaaactga tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca
      721 atgatcccat gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct
      781 gaagagacgt ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga
      841 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      901 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      961 ctggctcacc ttcgggtggg cctttctgcg tttata
//