LOCUS BBa_K557008 5028 bp DNA linear 01-Jun-2024 DEFINITION Reversed Toggle switch+Aptamer-CheZ+lasI-lasR ACCESSION BBa_K557008 VERSION BBa_K557008.1 FEATURES Location/Qualifiers misc_feature 1..2938 /label=BBa_K557004 misc_feature 2947..2992 /label=aptamer RBS 2993..3003 /label=rbs misc_feature 3004..3006 /label=start codon misc_feature 2947..3644 /label=BBa_K557001 misc_feature 1..3644 /label=BBa_K557005 misc_feature 3657..3660 /label=conserved RBS 3653..3664 /label=BBa_B0034 CDS 3671..4279 /label=lasI CDS 3671..4279 /label=BBa_C0178 misc_feature 4292..4295 /label=conserved RBS 4288..4299 /label=BBa_B0034 CDS 4306..5028 /label=BBa_C0179 misc_feature 3653..5028 /label=BBa_K557006 ORIGIN 1 ttattaagca ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta 61 gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg 121 aaccggtttt ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt 181 cagtttcaga cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc 241 ggtggaagct tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt 301 ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt 361 aacggtaaca acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg 421 gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc 481 gtactggaac tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac 541 tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc 601 gaactcgtga ccgttaacgg aaccttccat acgaactttg aaacgcatga actctttgat 661 aacgtcttcg gaggaagcca tctagtattt ctcctctttc actagatcag ccaaacgtct 721 cttcaggcca ctgactagcg ataactttcc ccacaacgga acaactctca ttgcatggga 781 tcattgggta ctgtgggttt agtggttgta aaaacacctg accgctatcc ctgatcagtt 841 tcttgaaggt aaactcatca cccccaagtc tggctatgca gaaatcacct ggctcaacag 901 cctgctcagg gtcaacgaga attaacattc cgtcaggaaa gcttggcttg gagcctgttg 961 gtgcggtcat ggaattacct tcaacctcaa gccagaatgc agaatcactg gcttttttgg 1021 ttgtgcttac ccatctctcc gcatcacctt tggtaaaggt tctaagctca ggtgagaaca 1081 tccctgcctg aacatgagaa aaaacagggt actcatactc acttctaagt gacggctgca 1141 tactaaccgc ttcatacatc tcgtagattt ctctggcgat tgaagggcta aattcttcaa 1201 cgctaacttt gagaattttt gcaagcaatg cggcgttata agcatttaat gcattgatgc 1261 cattaaataa agcaccaacg cctgactgcc ccatccccat cttgtctgcg acagattcct 1321 gggataagcc aagttcattt ttcttttttt cataaattgc tttaaggcga cgtgcgtcct 1381 caagctgctc ttgtgttaat ggtttctttt ttgtgctcat ttttttcctc ctactagaaa 1441 caaactttct tgtacatacg ttaaatctaa caaactttct tgtaaatatc taacaccgtg 1501 cgtgttgact attttacctc tggcggtgat aatggttgca ctagatacta aggaggttgt 1561 atgagtattt cttccagggt aaaaagcaaa agaatccagc ttggacttaa ccaggctgaa 1621 cttgctcaaa aggtggggac tacccagcag tctatagagc agctcgaaaa cggtaaaact 1681 aagcgaccac gctttttacc agaacttgcg tcagctcttg gcgtaagtgt tgactggctg 1741 ctcaatggca cctctgattc gaatgttaga tttgttgggc acgttgagcc caaagggaaa 1801 tatccattga ttagcatggt tagagctggt tcgtggtgtg aagcttgtga accctacgat 1861 atcaaggaca ttgatgaatg gtatgacagt gacgttaact tattaggcaa tggattctgg 1921 ctgaaggttg aaggtgattc catgacctca cctgtaggtc aaagcatccc tgaaggtcat 1981 atggtgttag tagatactgg acgggagcca gtgaatggaa gccttgttgt agccaaactg 2041 actgacgcga acgaagcaac attcaagaaa ctggtcatag atggcggtca gaagtacctg 2101 aaaggcctga atccttcatg gcctatgact cctatcaacg gaaactgcaa gattatcggt 2161 gttgtcgtgg aagcgagggt aaaattcgta taataaacta gaagaggaga aattaagcat 2221 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 2281 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 2341 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 2401 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 2461 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 2521 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 2581 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 2641 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 2701 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 2761 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 2821 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 2881 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata 2941 ctagagggtg ataccagcat cgtcttgatg cccttggcag caccccgctg caagacaaca 3001 agatgcaacc atcaatcaaa cctgctgacg agcattcagc tggcgatatc attgcgcgca 3061 tcggcagcct gacgcgtatg ctgcgcgaca gtttgcggga actggggctg gatcaggcca 3121 ttgccgaagc ggcggaagcc atccccgatg cgcgcgatcg tttgtactat gttgtgcaga 3181 tgaccgccca ggctgcggag cgggcgctga acagtgttga ggcgtcacaa ccgcatcagg 3241 atcaaatgga gaaatcagca aaagcgttaa cccaacgttg ggatgactgg tttgccgatc 3301 cgattgacct tgccgacgcc cgtgaactgg taacagatac acgacaattt ctggcagatg 3361 tacccgcgca taccagcttt actaacgcgc aactgctgga aatcatgatg gcgcaggatt 3421 ttcaggatct caccgggcag gtcattaagc ggatgatgga tgtcattcag gagatcgaac 3481 gccagttgct gatggtgctg ttggaaaaca tcccggaaca ggagtcgcgt ccaaaacgtg 3541 aaaaccagag tttgcttaat ggacctcagg tcgataccag caaagccggt gtggtagcca 3601 gtcaggatca ggtggacgat ttgttggata gacttggatt ttgatactag agaaagagga 3661 gaaatactag atgatcgttc agatcggtcg tcgtgaagag ttcgacaaaa aactgctggg 3721 tgaaatgcac aaactgcgtg ctcaggtttt caaagaacgt aaaggttggg acgtttccgt 3781 tatcgacgaa atggaaatcg acggttacga cgctctgtcc ccgtactaca tgctgatcca 3841 ggaagacacc ccggaagctc aggttttcgg ttgctggcgt atcttcgaca ccaccggtcc 3901 gtacatgctg aaaaacacct tcccggaact gctgcacggt aaagaagctc cgtgctcccc 3961 gcacatctgg gaactgtccc gtttcgctat caactccggt cagaaaggtt ccctgggttt 4021 ctccgactgc accctggaag ctatgcgtgc tctggctcgt tactccttgc agaacgacat 4081 ccagaccctg gttaccgtta ccaccgttgg tgttgaaaaa atgatgatcc gtgctggtct 4141 ggacgtttcc cgtttcggtc cgcacctgaa aatcggtatc gaacgtgctg ttgctctgcg 4201 tatcgaactg aacgctaaaa cccagatcgc tctgtacggt ggtgttctgg ttgaacagcg 4261 tctggctgtt tcctaataat actagagaaa gaggagaaat actagatggc cttggttgac 4321 ggttttcttg agctggaacg ctcaagtgga aaattggagt ggagcgccat cctccagaag 4381 atggcgagcg accttggatt ctcgaagatc ctgttcggcc tgttgcctaa ggacagccag 4441 gactacgaga acgccttcat cgtcggcaac tacccggccg cctggcgcga gcattacgac 4501 cgggctggct acgcgcgggt cgacccgacg gtcagtcact gtacccagag cgtactgccg 4561 attttctggg aaccgtccat ctaccagacg cgaaagcagc acgagttctt cgaggaagcc 4621 tcggccgccg gcctggtgta tgggctgacc atgccgctgc atggtgctcg cggcgaactc 4681 ggcgcgctga gcctcagcgt ggaagcggaa aaccgggccg aggccaaccg tttcatagag 4741 tcggtcctgc cgaccctgtg gatgctcaag gactacgcac tgcaaagcgg tgccggactg 4801 gccttcgaac atccggtcag caaaccggtg gttctgacca gccgggagaa ggaagtgttg 4861 cagtggtgcg ccatcggcaa gaccagttgg gagatatcgg ttatctgcaa ctgctcggaa 4921 gccaatgtga acttccatat gggaaatatt cggcggaagt tcggtgtgac ctcccgccgc 4981 gtagcggcca ttatggccgt taatttgggt cttattactc tctaataa //