BBa_K557008
type: ComponentDefinition
persistent identity: BBa K557008
display id: BBa_K557008
version: 1
was generated by: iGEM to SBOL conversion
description: Reversed Toggle switch+Aptamer-CheZ+lasI-lasR
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The part is made up of the standard parts from partsregistry.
owned by: james
owned by: myers
mutable description: This part is composed of improved device in automatically division and quorum sensing parts which will form a negative feedback and helps the forming of the second colony.
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Yinghong Lan Siwei Luo
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K557006
component: BBa_K557005
sequence annotation: BBa_K557005
sequence annotation: BBa_K557006
sequence: https://synbiohub.org/public/igem/BBa_K557008_sequence/1

BBa_K557006
type: Component
persistent identity: component2149381
display id: component2149381
version: 1
top level: BBa_K557008
definition: BBa_K557006
access: public

BBa_K557005
type: Component
persistent identity: component2149373
display id: component2149373
version: 1
top level: BBa_K557008
definition: BBa_K557005
access: public

BBa_K557005
type: SequenceAnnotation
persistent identity: annotation2149373
display id: annotation2149373
version: 1
top level: BBa_K557008
location: https://synbiohub.org/public/igem/BBa_K557008/annotation2149373/range2149373/1
component: BBa_K557005

https://synbiohub.org/public/igem/BBa_K557008/annotation2149373/range2149373/1
type: Range
persistent identity: range2149373
display id: range2149373
version: 1
top level: BBa_K557008
direction: direction/reverse
start: 1
end: 3644
orientation: inline

BBa_K557006
type: SequenceAnnotation
persistent identity: annotation2149381
display id: annotation2149381
version: 1
top level: BBa_K557008
location: https://synbiohub.org/public/igem/BBa_K557008/annotation2149381/range2149381/1
component: BBa_K557006

https://synbiohub.org/public/igem/BBa_K557008/annotation2149381/range2149381/1
type: Range
persistent identity: range2149381
display id: range2149381
version: 1
top level: BBa_K557008
direction: direction/reverse
start: 3653
end: 5028
orientation: inline

BBa_K557005
type: ComponentDefinition
persistent identity: BBa K557005
display id: BBa_K557005
version: 1
was generated by: iGEM to SBOL conversion
description: Reversed Toggle switch+Aptamer-CheZ
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The part is made up of the standard parts from partsregistry.
owned by: james
owned by: myers
mutable description: This part resulted from the results of experience with part BBa_K557002. More details could be read on the page of BBa_K557002. Given that we expected higher ratios of bacteria moving away, we inserted aptamer-CheZ in the opposite side of the toggle switch.
discontinued: false
dominant: true
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: NONE
star: false
creator: Yinghong Lan
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K557001
component: BBa_K557004
sequence annotation: BBa_K557004
sequence annotation: BBa_K557001
sequence: https://synbiohub.org/public/igem/BBa_K557005_sequence/1

BBa_K557001
type: Component
persistent identity: component2149353
display id: component2149353
version: 1
top level: BBa_K557005
definition: BBa_K557001
access: public

BBa_K557004
type: Component
persistent identity: component2149349
display id: component2149349
version: 1
top level: BBa_K557005
definition: BBa_K557004
access: public

BBa_K557004
type: SequenceAnnotation
persistent identity: annotation2149349
display id: annotation2149349
version: 1
top level: BBa_K557005
location: https://synbiohub.org/public/igem/BBa_K557005/annotation2149349/range2149349/1
component: BBa_K557004

https://synbiohub.org/public/igem/BBa_K557005/annotation2149349/range2149349/1
type: Range
persistent identity: range2149349
display id: range2149349
version: 1
top level: BBa_K557005
direction: direction/reverse
start: 1
end: 2938
orientation: inline

BBa_K557001
type: SequenceAnnotation
persistent identity: annotation2149353
display id: annotation2149353
version: 1
top level: BBa_K557005
location: https://synbiohub.org/public/igem/BBa_K557005/annotation2149353/range2149353/1
component: BBa_K557001

https://synbiohub.org/public/igem/BBa_K557005/annotation2149353/range2149353/1
type: Range
persistent identity: range2149353
display id: range2149353
version: 1
top level: BBa_K557005
direction: direction/reverse
start: 2947
end: 3644
orientation: inline

BBa_K557006
type: ComponentDefinition
persistent identity: BBa K557006
display id: BBa_K557006
version: 1
was generated by: iGEM to SBOL conversion
description: lasI+lasR
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The part is made up of the standard parts from partsregistry.
owned by: james
owned by: myers
mutable description: This part is a translational unit of BBa_K557008 and is constructed of two essential parts in quorum sensing: lasI protein, which produces the chemical signal AI-1, and lasR protein, which accepts chemical signal AI-1. Combined with the lasbox in BBa_K557007, it will form a negative feedback which helps better patterning of the automatically division of bacterial colonies.
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: NONE
star: false
creator: Yinghong Lan
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0179
component: BBa_B0034
component: BBa_C0178
sequence annotation: BBa_B0034
sequence annotation: BBa_C0178
sequence annotation: BBa_C0179
sequence: https://synbiohub.org/public/igem/BBa_K557006_sequence/1

BBa_C0179
type: Component
persistent identity: component2149360
display id: component2149360
version: 1
top level: BBa_K557006
definition: lasR
access: public

BBa_B0034
type: Component
persistent identity: component2149359
display id: component2149359
version: 1
top level: BBa_K557006
definition: BBa_B0034
access: public

BBa_C0178
type: Component
persistent identity: component2149357
display id: component2149357
version: 1
top level: BBa_K557006
definition: lasI
access: public

BBa_B0034
type: Component
persistent identity: component2149355
display id: component2149355
version: 1
top level: BBa_K557006
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2149355
display id: annotation2149355
version: 1
top level: BBa_K557006
location: https://synbiohub.org/public/igem/BBa_K557006/annotation2149355/range2149355/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K557006/annotation2149355/range2149355/1
type: Range
persistent identity: range2149355
display id: range2149355
version: 1
top level: BBa_K557006
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation2149357
display id: annotation2149357
version: 1
top level: BBa_K557006
location: https://synbiohub.org/public/igem/BBa_K557006/annotation2149357/range2149357/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_K557006/annotation2149357/range2149357/1
type: Range
persistent identity: range2149357
display id: range2149357
version: 1
top level: BBa_K557006
direction: direction/reverse
start: 19
end: 627
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2149359
display id: annotation2149359
version: 1
top level: BBa_K557006
location: https://synbiohub.org/public/igem/BBa_K557006/annotation2149359/range2149359/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K557006/annotation2149359/range2149359/1
type: Range
persistent identity: range2149359
display id: range2149359
version: 1
top level: BBa_K557006
direction: direction/reverse
start: 636
end: 647
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2149360
display id: annotation2149360
version: 1
top level: BBa_K557006
location: https://synbiohub.org/public/igem/BBa_K557006/annotation2149360/range2149360/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K557006/annotation2149360/range2149360/1
type: Range
persistent identity: range2149360
display id: range2149360
version: 1
top level: BBa_K557006
direction: direction/reverse
start: 654
end: 1376
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0178
display id: BBa_C0178
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0078 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: lasI
sequence: https://synbiohub.org/public/igem/BBa_C0178_sequence/1

lasI
type: SequenceAnnotation
persistent identity: annotation1937124
display id: annotation1937124
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1
type: Range
persistent identity: range1937124
display id: range1937124
version: 1
top level: lasI
direction: direction/forward
start: 1
end: 609
orientation: inline

BBa_K557001
type: ComponentDefinition
persistent identity: BBa K557001
display id: BBa_K557001
version: 1
was generated by: iGEM to SBOL conversion
description: Aptamer-CheZ: theophylline-sensitive synthetic riboswitch+phosphatase which dephosphorylates CheY-P
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: Escherichia coli, the strain has always been kept in the lab of USTC-IGEM
owned by: james
owned by: myers
mutable description: This part is composed of a theophylline-sensitive aptamer,which will pair the ribosome binding site of the downstream gene in the absence of theophylline and thus inhibit the translation of the gene while expose the ribosome binding site in the presence of theophylline, and the CheZ gene, which is a phosphatase which dephosphorylates CheY-P and causes the flagellum to rotate counterclockwise and is essential for keeping the motility of E.coli. Thus this part provides a ligand-inducible motility and makes it possible to guide E.coli by theophylline.
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We found the sequence of this part in literature and tried to design primers to construct it. But we were faced with a challenging problem in primer design, which comes from the basic properties of the aptamer involved. The aptamer tends to pair the ribosome binding site in the absence of theophylline, which is true in PCR, and enables primers to pair to the corresponding parts and the new strand to elongate. We made contact with the author of our reference and found they took different strategies in assembling the aptamer to CheZ instead of PCR. But thanks to the warm help of Professor J. S. Parkinson and Professor J. P. Gallivan, we acquired the sequence of primers used in assembling the aptamer to another gene and designed our own after analyzing them: forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGGGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACCCCGCTGCAAGACAACAAG ATGCAACCATCAATCAAACC-3' reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTATTATTAAAATCCAAGACTATCCAACAAATCGT-3' Besides, we used special protocal of PCR: 96℃ 10min 96℃ 30s, 56℃ 30s, 72℃ 1min for 16 cycles and each cycle the annealing temperature increases by 1℃ 96℃ 30s, 72℃ 30s, 72℃ 1min for 20 cycles 72℃ 10min
star: false
creator: Yinghong Lan
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: aptamer
sequence annotation: rbs
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K557001_sequence/1

aptamer
type: SequenceAnnotation
persistent identity: annotation2149326
display id: annotation2149326
version: 1
top level: BBa_K557001
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149326/range2149326/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K557001/annotation2149326/range2149326/1
type: Range
persistent identity: range2149326
display id: range2149326
version: 1
top level: BBa_K557001
direction: direction/forward
start: 1
end: 46
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2149327
display id: annotation2149327
version: 1
top level: BBa_K557001
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149327/range2149327/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K557001/annotation2149327/range2149327/1
type: Range
persistent identity: range2149327
display id: range2149327
version: 1
top level: BBa_K557001
direction: direction/forward
start: 47
end: 57
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2149328
display id: annotation2149328
version: 1
top level: BBa_K557001
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149328/range2149328/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K557001/annotation2149328/range2149328/1
type: Range
persistent identity: range2149328
display id: range2149328
version: 1
top level: BBa_K557001
direction: direction/forward
start: 58
end: 60
orientation: inline

BBa_K557004
type: ComponentDefinition
persistent identity: BBa K557004
display id: BBa_K557004
version: 1
was generated by: iGEM to SBOL conversion
description: Reversed Toggle switch for the convenience of insertion of genes
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The part is made by PCR of the part BBa_K228003.
owned by: james
owned by: myers
mutable description: This part is the reverse version of toggle switch so that with the standard prefix and suffix it's more convenient to insert genes on both sides of toggle switch.
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Yinghong Lan
type: DnaRegion
role: SO:0000804
role: partType/Device
sequence: https://synbiohub.org/public/igem/BBa_K557004_sequence/1

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0079 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

https://synbiohub.org/public/igem/BBa_K557008_sequence/1
type: Sequence
persistent identity: BBa K557008 sequence
display id: BBa_K557008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K557005_sequence/1
type: Sequence
persistent identity: BBa K557005 sequence
display id: BBa_K557005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K557006_sequence/1
type: Sequence
persistent identity: BBa K557006 sequence
display id: BBa_K557006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttcctaataatactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0178_sequence/1
type: Sequence
persistent identity: BBa C0178 sequence
display id: BBa_C0178_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttcctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K557001_sequence/1
type: Sequence
persistent identity: BBa K557001 sequence
display id: BBa_K557001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagacttggattttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K557004_sequence/1
type: Sequence
persistent identity: BBa K557004 sequence
display id: BBa_K557004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0179_sequence/1
type: Sequence
persistent identity: BBa C0179 sequence
display id: BBa_C0179_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z