BBa_K557010
1
BBa_K557010
ColicinE7+Toggle-switch+Aptamer-CheZ+lysis
2011-10-01T11:00:00Z
2015-05-08T01:12:41Z
The part is made up of the standard parts from partsregistry.
AIIS. To be continued...
false
false
_725_
0
8574
9
It's complicated
true
None
false
Yinghong Lan Siwei Luo
component2220858
1
BBa_K557009
component2220839
1
BBa_K117001
annotation2220839
1
BBa_K117001
range2220839
1
1
1899
annotation2220858
1
BBa_K557009
range2220858
1
1908
5701
BBa_K117001
1
BBa_K117001
ColicinE7 with Immunity
2008-10-06T11:00:00Z
2015-05-08T01:09:34Z
to be updated
This gene sequence once expressed will produce colicin E7. This is an endonuclease with random cutting site and can kill various strains of EHEC bacteria. However, inside the host cell, immediatle after being produced, its toxicity is inhibited by Immunity protein (which is also expressed at the same time). These 2 proteins form a complex which is harmless to the host cell.
Under SOS response, the host cell produces lysis protein, which cause the cell to lyze. At the same time, lysis protein remove Immunity protein from the complex with colE7. As a result, colE7 regains its toxicity and ability to kill the EHEC bacteria nearby.
For information on lysis gene, please refer to our BBa_K117000 part.
false
false
_209_
0
2774
9
It's complicated
true
to be updated
true
Nguyen Xuan Hung
annotation1989190
1
caeE7 toxicity domain
range1989190
1
478
587
annotation1989189
1
caeE7 RBS
range1989189
1
117
124
annotation1989191
1
Immunity (ceiE7)
range1989191
1
1862
1899
BBa_K557001
1
BBa_K557001
Aptamer-CheZ: theophylline-sensitive synthetic riboswitch+phosphatase which dephosphorylates CheY-P
2011-10-01T11:00:00Z
2015-05-08T01:12:41Z
Escherichia coli, the strain has always been kept in the lab of USTC-IGEM
This part is composed of a theophylline-sensitive aptamer,which will pair the ribosome binding site of the downstream gene in the absence of theophylline and thus inhibit the translation of the gene while expose the ribosome binding site in the presence of theophylline, and the CheZ gene, which is a phosphatase which dephosphorylates CheY-P and causes the flagellum to rotate counterclockwise and is essential for keeping the motility of E.coli. Thus this part provides a ligand-inducible motility and makes it possible to guide E.coli by theophylline.
false
false
_725_
0
8574
9
It's complicated
true
We found the sequence of this part in literature and tried to design primers to construct it. But we were faced with a challenging problem in primer design, which comes from the basic properties of the aptamer involved. The aptamer tends to pair the ribosome binding site in the absence of theophylline, which is true in PCR, and enables primers to pair to the corresponding parts and the new strand to elongate. We made contact with the author of our reference and found they took different strategies in assembling the aptamer to CheZ instead of PCR. But thanks to the warm help of Professor J. S. Parkinson and Professor J. P. Gallivan, we acquired the sequence of primers used in assembling the aptamer to another gene and designed our own after analyzing them:
<html>
<div style="padding-left:25px;background-color:#FFFACD">
<p>forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGGGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACCCCGCTGCAAGACAACAAG ATGCAACCATCAATCAAACC-3'</p>
<p>reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTATTATTAAAATCCAAGACTATCCAACAAATCGT-3'</p>
</div>
</html>
Besides, we used special protocal of PCR:
96℃ 10min
96℃ 30s, 56℃ 30s, 72℃ 1min for 16 cycles and each cycle the annealing temperature increases by 1℃
96℃ 30s, 72℃ 30s, 72℃ 1min for 20 cycles
72℃ 10min
false
Yinghong Lan
annotation2149328
1
start codon
range2149328
1
58
60
annotation2149326
1
aptamer
range2149326
1
1
46
annotation2149327
1
rbs
range2149327
1
47
57
BBa_K117000
1
BBa_K117000
Lysis gene (promotes lysis in colicin-producing bacteria strain)
2008-10-06T11:00:00Z
2015-05-08T01:09:34Z
to be updated
Released HQ 2013
This lysis gene encodes for the lysis protein in colicin-producing strains of bacteria. Once activated, it causes the host cell to lyze. It also removes the immunity protein out of colicin, and hence, activates the endonuclease activity of the colicin.
false
false
_209_
0
2774
9
In stock
true
to be updated
true
Nguyen Xuan Hung
BBa_K557009
1
BBa_K557009
Toggle-switch+Aptamer+CheZ+Lysis
2011-10-01T11:00:00Z
2015-05-08T01:12:41Z
The part is made up of the standard parts from partsregistry.
Part of AIIS. To be continued....
false
false
_725_
0
8574
9
It's complicated
false
None
false
Yinghong Lan
component2220025
1
BBa_K557002
component2220026
1
BBa_K117000
annotation2220026
1
BBa_K117000
range2220026
1
3651
3794
annotation2220025
1
BBa_K557002
range2220025
1
1
3644
BBa_K557002
1
BBa_K557002
Toggle-switch+Aptamer+CheZ
2011-10-01T11:00:00Z
2015-05-08T01:12:41Z
The part is made up of the standard parts from partsregistry.
This part is the assembly of riboswitches followed by an important gene CheZ in keeping E.coli motile (BBa_K557001) finely tuned by small molecules and toggle switches (BBa_K228003) pushed on and off while memorizing current state. It would make it possible that bacterial colonies will automatically divide into two parts after certain time, during which riboswitches would act as the main power to drive two parts away from each other while toggle switches would play the role of giving birth to two 'different' kinds of bacteria in one colony.
false
true
_725_
0
8574
9
It's complicated
true
None
false
Yinghong Lan
component2219057
1
BBa_K557001
component2219053
1
BBa_K228003
annotation2219053
1
BBa_K228003
range2219053
1
1
2938
annotation2219057
1
BBa_K557001
range2219057
1
2947
3644
BBa_K228003
1
BBa_K228003
Bistable part
2009-09-05T11:00:00Z
2015-05-08T01:11:32Z
The part is made up of the standard parts from partsregistry, and some of the sub-parts are inverted by PCR.
It is a part which has two stable states--CI and CI434 and the orginal state can be either of the two. CI repressor is expressed after a CI434 repressible promoter, while the CI434 is expressed after a CI repressible promoter, thus the system can only stay in one state, when one is expressed and the other is repressed. The two states can be swicthed in certain conditions. In other words, the orginal state can be changed by exogenetic triggering. For example, the orginal state is CI, then you can use an gene which exogenetically expresses CI434 to trigger the bistable part into the state of CI434.
false
true
_353_
0
4403
9
It's complicated
true
false
Shan Shen
annotation2028125
1
rbs
range2028125
1
2203
2211
annotation2035614
1
rbs
range2035614
1
1546
1554
annotation2028122
1
mRFP
range2028122
1
1
681
annotation2028128
1
rbs
range2028128
1
1426
1432
annotation2028124
1
CI434 repressor
range2028124
1
1561
2196
annotation2035764
1
CI434 regulated promoter
range2035764
1
1439
1490
annotation2035515
1
CI regulated promoter
range2035515
1
1491
1539
annotation2035763
1
CI434 OR1
range2035763
1
1439
1454
annotation2028123
1
eGFP
range2028123
1
2219
2938
annotation2028127
1
rbs
range2028127
1
688
700
annotation2028126
1
lamda CI repressor
range2028126
1
707
1420
BBa_K228003_sequence
1
ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataaactagaagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
BBa_K557002_sequence
1
ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataaactagaagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagacttggattttga
BBa_K557009_sequence
1
ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataaactagaagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagacttggattttgatactagatgaaaaaaataacagggattattttattgcttcttgcagccattattcttgctgcatgtcaggcaaactatatccgtgatgttcagggcgggacagtatcaccgtcgtcaactgctgaactgaccggagtggaaacgcagtaa
BBa_K557001_sequence
1
ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagacttggattttga
BBa_K117000_sequence
1
atgaaaaaaataacagggattattttattgcttcttgcagccattattcttgctgcatgtcaggcaaactatatccgtgatgttcagggcgggacagtatcaccgtcgtcaactgctgaactgaccggagtggaaacgcagtaa
BBa_K117001_sequence
1
aatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtacagtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaacagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggctccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatggcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgctttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatctttgcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaagatgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttccgctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagttgcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctggtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataaaggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagtgggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaacgcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaatcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaacttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatggctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgcatttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaaaataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaaaacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaactacgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagtaaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcagggaagagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgtggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacagaggc
BBa_K557010_sequence
1
aatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtacagtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaacagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggctccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatggcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgctttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatctttgcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaagatgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttccgctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagttgcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctggtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataaaggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagtgggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaacgcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaatcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaacttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatggctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgcatttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaaaataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaaaacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaactacgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagtaaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcagggaagagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgtggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacagaggctactagagttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataaactagaagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagacttggattttgatactagatgaaaaaaataacagggattattttattgcttcttgcagccattattcttgctgcatgtcaggcaaactatatccgtgatgttcagggcgggacagtatcaccgtcgtcaactgctgaactgaccggagtggaaacgcagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z