##gff-version 3
##sequence-region BBa_K557010 1 5701
BBa_K557010	.	engineered_region	1	1899	.	+	0	ID=BBa_K117001;Name=BBa_K117001
BBa_K557010	.	CDS	1862	1899	.	+	0	ID=annotation1989191;Name=Immunity (ceiE7);Parent=BBa_K117001
BBa_K557010	.	sequence_feature	478	587	.	+	0	ID=annotation1989190;Name=caeE7 toxicity domain;Parent=BBa_K117001
BBa_K557010	.	ribosome_entry_site	117	124	.	+	0	ID=annotation1989189;Name=caeE7 RBS;Parent=BBa_K117001
BBa_K557010	.	engineered_region	1908	5701	.	+	0	ID=BBa_K557009;Name=BBa_K557009
BBa_K557010	.	engineered_region	1908	5551	.	+	0	ID=BBa_K557002;Name=BBa_K557002;Parent=BBa_K557009
BBa_K557010	.	engineered_region	1908	4845	.	+	0	ID=BBa_K228003;Name=BBa_K228003;Parent=BBa_K557002
BBa_K557010	.	ribosome_entry_site	4110	4118	.	+	0	ID=annotation2028125;Name=rbs;Parent=BBa_K228003
BBa_K557010	.	promoter	3346	3397	.	+	0	ID=annotation2035764;Name=CI434 regulated promoter;Parent=BBa_K228003
BBa_K557010	.	ribosome_entry_site	3453	3461	.	+	0	ID=annotation2035614;Name=rbs;Parent=BBa_K228003
BBa_K557010	.	CDS	3468	4103	.	+	0	ID=annotation2028124;Name=CI434 repressor;Parent=BBa_K228003
BBa_K557010	.	CDS	2614	3327	.	+	0	ID=annotation2028126;Name=lamda CI repressor;Parent=BBa_K228003
BBa_K557010	.	ribosome_entry_site	3333	3339	.	+	0	ID=annotation2028128;Name=rbs;Parent=BBa_K228003
BBa_K557010	.	promoter	3398	3446	.	+	0	ID=annotation2035515;Name=CI regulated promoter;Parent=BBa_K228003
BBa_K557010	.	non_covalent_binding_site	3346	3361	.	+	0	ID=annotation2035763;Name=CI434 OR1;Parent=BBa_K228003
BBa_K557010	.	CDS	4126	4845	.	+	0	ID=annotation2028123;Name=eGFP;Parent=BBa_K228003
BBa_K557010	.	CDS	1908	2588	.	+	0	ID=annotation2028122;Name=mRFP;Parent=BBa_K228003
BBa_K557010	.	ribosome_entry_site	2595	2607	.	+	0	ID=annotation2028127;Name=rbs;Parent=BBa_K228003
BBa_K557010	.	engineered_region	4854	5551	.	+	0	ID=BBa_K557001;Name=BBa_K557001;Parent=BBa_K557002
BBa_K557010	.	non_covalent_binding_site	4854	4899	.	+	0	ID=annotation2149326;Name=aptamer;Parent=BBa_K557001
BBa_K557010	.	start_codon	4911	4913	.	+	0	ID=annotation2149328;Name=start codon;Parent=BBa_K557001
BBa_K557010	.	ribosome_entry_site	4900	4910	.	+	0	ID=annotation2149327;Name=rbs;Parent=BBa_K557001
BBa_K557010	.	CDS	5558	5701	.	+	0	ID=BBa_K117000;Name=BBa_K117000;Parent=BBa_K557009
>BBa_K557010
aatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtac
agtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaa
cagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggc
tccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatg
gcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgc
tttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatcttt
gcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaag
atgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttcc
gctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagtt
gcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctg
gtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataa
aggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagt
gggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaac
gcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaa
tcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaa
cttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatgg
ctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgc
atttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaa
aataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaa
aacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaact
acgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagt
aaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcaggga
agagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgt
ggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacaga
ggctactagagttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgt
tgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggt
tttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttcc
gggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtac
cacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttc
gaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgttta
acgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactt
tcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacgga
accttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttc
actagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatggg
atcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcat
cacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcagg
aaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggctttt
ttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacat
gagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctct
ggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgca
ttgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaa
gttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttt
tgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatc
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatt
tcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagc
agtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaag
tgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatcca
ttgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatg
acagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaag
catccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgact
gacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggc
ctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataaactag
aagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacgg
cgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttc
atctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagcc
gctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatctt
cttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctg
aagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctata
tcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagct
cgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccag
tccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactc
tcggcatggacgagctgtacaagtaatactagagggtgataccagcatcgtcttgatgcccttggcagcaccccgctgc
aagacaacaagatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcct
gacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgat
gcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcac
aaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattga
ccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaac
gcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtca
ttcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaa
ccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttg
ttggatagacttggattttgatactagatgaaaaaaataacagggattattttattgcttcttgcagccattattcttg
ctgcatgtcaggcaaactatatccgtgatgttcagggcgggacagtatcaccgtcgtcaactgctgaactgaccggagt
ggaaacgcagtaa