BBa_K557010
type: ComponentDefinition
persistent identity: BBa K557010
display id: BBa_K557010
version: 1
was generated by: iGEM to SBOL conversion
description: ColicinE7+Toggle-switch+Aptamer-CheZ+lysis
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The part is made up of the standard parts from partsregistry.
owned by: james
owned by: myers
mutable description: AIIS. To be continued...
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None
star: false
creator: Yinghong Lan Siwei Luo
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K557009
component: BBa_K117001
sequence annotation: BBa_K117001
sequence annotation: BBa_K557009
sequence: https://synbiohub.org/public/igem/BBa_K557010_sequence/1

BBa_K557009
type: Component
persistent identity: component2220858
display id: component2220858
version: 1
top level: BBa_K557010
definition: BBa_K557009
access: public

BBa_K117001
type: Component
persistent identity: component2220839
display id: component2220839
version: 1
top level: BBa_K557010
definition: BBa_K117001
access: public

BBa_K117001
type: SequenceAnnotation
persistent identity: annotation2220839
display id: annotation2220839
version: 1
top level: BBa_K557010
location: https://synbiohub.org/public/igem/BBa_K557010/annotation2220839/range2220839/1
component: BBa_K117001

https://synbiohub.org/public/igem/BBa_K557010/annotation2220839/range2220839/1
type: Range
persistent identity: range2220839
display id: range2220839
version: 1
top level: BBa_K557010
direction: direction/reverse
start: 1
end: 1899
orientation: inline

BBa_K557009
type: SequenceAnnotation
persistent identity: annotation2220858
display id: annotation2220858
version: 1
top level: BBa_K557010
location: https://synbiohub.org/public/igem/BBa_K557010/annotation2220858/range2220858/1
component: BBa_K557009

https://synbiohub.org/public/igem/BBa_K557010/annotation2220858/range2220858/1
type: Range
persistent identity: range2220858
display id: range2220858
version: 1
top level: BBa_K557010
direction: direction/reverse
start: 1908
end: 5701
orientation: inline

BBa_K117001
type: ComponentDefinition
persistent identity: BBa K117001
display id: BBa_K117001
version: 1
was generated by: iGEM to SBOL conversion
description: ColicinE7 with Immunity
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
mutable provenance: to be updated
owned by: james
owned by: myers
mutable description: This gene sequence once expressed will produce colicin E7. This is an endonuclease with random cutting site and can kill various strains of EHEC bacteria. However, inside the host cell, immediatle after being produced, its toxicity is inhibited by Immunity protein (which is also expressed at the same time). These 2 proteins form a complex which is harmless to the host cell. Under SOS response, the host cell produces lysis protein, which cause the cell to lyze. At the same time, lysis protein remove Immunity protein from the complex with colE7. As a result, colE7 regains its toxicity and ability to kill the EHEC bacteria nearby. For information on lysis gene, please refer to our BBa_K117000 part.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: to be updated
star: true
creator: Nguyen Xuan Hung
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: caeE7 RBS
sequence annotation: caeE7 toxicity domain
sequence annotation: Immunity (ceiE7)
sequence: https://synbiohub.org/public/igem/BBa_K117001_sequence/1

caeE7 RBS
type: SequenceAnnotation
persistent identity: annotation1989189
display id: annotation1989189
version: 1
top level: BBa_K117001
location: https://synbiohub.org/public/igem/BBa_K117001/annotation1989189/range1989189/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K117001/annotation1989189/range1989189/1
type: Range
persistent identity: range1989189
display id: range1989189
version: 1
top level: BBa_K117001
direction: direction/forward
start: 117
end: 124
orientation: inline

caeE7 toxicity domain
type: SequenceAnnotation
persistent identity: annotation1989190
display id: annotation1989190
version: 1
top level: BBa_K117001
location: https://synbiohub.org/public/igem/BBa_K117001/annotation1989190/range1989190/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K117001/annotation1989190/range1989190/1
type: Range
persistent identity: range1989190
display id: range1989190
version: 1
top level: BBa_K117001
direction: direction/forward
start: 478
end: 587
orientation: inline

Immunity (ceiE7)
type: SequenceAnnotation
persistent identity: annotation1989191
display id: annotation1989191
version: 1
top level: BBa_K117001
location: https://synbiohub.org/public/igem/BBa_K117001/annotation1989191/range1989191/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K117001/annotation1989191/range1989191/1
type: Range
persistent identity: range1989191
display id: range1989191
version: 1
top level: BBa_K117001
direction: direction/forward
start: 1862
end: 1899
orientation: inline

BBa_K557009
type: ComponentDefinition
persistent identity: BBa K557009
display id: BBa_K557009
version: 1
was generated by: iGEM to SBOL conversion
description: Toggle-switch+Aptamer+CheZ+Lysis
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The part is made up of the standard parts from partsregistry.
owned by: james
owned by: myers
mutable description: Part of AIIS. To be continued....
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Yinghong Lan
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K117000
component: BBa_K557002
sequence annotation: BBa_K557002
sequence annotation: BBa_K117000
sequence: https://synbiohub.org/public/igem/BBa_K557009_sequence/1

BBa_K117000
type: Component
persistent identity: component2220026
display id: component2220026
version: 1
top level: BBa_K557009
definition: BBa_K117000
access: public

BBa_K557002
type: Component
persistent identity: component2220025
display id: component2220025
version: 1
top level: BBa_K557009
definition: BBa_K557002
access: public

BBa_K557002
type: SequenceAnnotation
persistent identity: annotation2220025
display id: annotation2220025
version: 1
top level: BBa_K557009
location: https://synbiohub.org/public/igem/BBa_K557009/annotation2220025/range2220025/1
component: BBa_K557002

https://synbiohub.org/public/igem/BBa_K557009/annotation2220025/range2220025/1
type: Range
persistent identity: range2220025
display id: range2220025
version: 1
top level: BBa_K557009
direction: direction/reverse
start: 1
end: 3644
orientation: inline

BBa_K117000
type: SequenceAnnotation
persistent identity: annotation2220026
display id: annotation2220026
version: 1
top level: BBa_K557009
location: https://synbiohub.org/public/igem/BBa_K557009/annotation2220026/range2220026/1
component: BBa_K117000

https://synbiohub.org/public/igem/BBa_K557009/annotation2220026/range2220026/1
type: Range
persistent identity: range2220026
display id: range2220026
version: 1
top level: BBa_K557009
direction: direction/reverse
start: 3651
end: 3794
orientation: inline

BBa_K117000
type: ComponentDefinition
persistent identity: BBa K117000
display id: BBa_K117000
version: 1
was generated by: iGEM to SBOL conversion
description: Lysis gene (promotes lysis in colicin-producing bacteria strain)
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
mutable provenance: to be updated
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This lysis gene encodes for the lysis protein in colicin-producing strains of bacteria. Once activated, it causes the host cell to lyze. It also removes the immunity protein out of colicin, and hence, activates the endonuclease activity of the colicin.
discontinued: false
dominant: false
experience: experience/Works
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: to be updated
star: true
creator: Nguyen Xuan Hung
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K117000_sequence/1

BBa_K557002
type: ComponentDefinition
persistent identity: BBa K557002
display id: BBa_K557002
version: 1
was generated by: iGEM to SBOL conversion
description: Toggle-switch+Aptamer+CheZ
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The part is made up of the standard parts from partsregistry.
owned by: james
owned by: myers
mutable description: This part is the assembly of riboswitches followed by an important gene CheZ in keeping E.coli motile (BBa_K557001) finely tuned by small molecules and toggle switches (BBa_K228003) pushed on and off while memorizing current state. It would make it possible that bacterial colonies will automatically divide into two parts after certain time, during which riboswitches would act as the main power to drive two parts away from each other while toggle switches would play the role of giving birth to two 'different' kinds of bacteria in one colony.
discontinued: false
dominant: true
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None
star: false
creator: Yinghong Lan
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K557001
component: BBa_K228003
sequence annotation: BBa_K228003
sequence annotation: BBa_K557001
sequence: https://synbiohub.org/public/igem/BBa_K557002_sequence/1

BBa_K557001
type: Component
persistent identity: component2219057
display id: component2219057
version: 1
top level: BBa_K557002
definition: BBa_K557001
access: public

BBa_K228003
type: Component
persistent identity: component2219053
display id: component2219053
version: 1
top level: BBa_K557002
definition: BBa_K228003
access: public

BBa_K228003
type: SequenceAnnotation
persistent identity: annotation2219053
display id: annotation2219053
version: 1
top level: BBa_K557002
location: https://synbiohub.org/public/igem/BBa_K557002/annotation2219053/range2219053/1
component: BBa_K228003

https://synbiohub.org/public/igem/BBa_K557002/annotation2219053/range2219053/1
type: Range
persistent identity: range2219053
display id: range2219053
version: 1
top level: BBa_K557002
direction: direction/reverse
start: 1
end: 2938
orientation: inline

BBa_K557001
type: SequenceAnnotation
persistent identity: annotation2219057
display id: annotation2219057
version: 1
top level: BBa_K557002
location: https://synbiohub.org/public/igem/BBa_K557002/annotation2219057/range2219057/1
component: BBa_K557001

https://synbiohub.org/public/igem/BBa_K557002/annotation2219057/range2219057/1
type: Range
persistent identity: range2219057
display id: range2219057
version: 1
top level: BBa_K557002
direction: direction/reverse
start: 2947
end: 3644
orientation: inline

BBa_K557001
type: ComponentDefinition
persistent identity: BBa K557001
display id: BBa_K557001
version: 1
was generated by: iGEM to SBOL conversion
description: Aptamer-CheZ: theophylline-sensitive synthetic riboswitch+phosphatase which dephosphorylates CheY-P
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: Escherichia coli, the strain has always been kept in the lab of USTC-IGEM
owned by: james
owned by: myers
mutable description: This part is composed of a theophylline-sensitive aptamer,which will pair the ribosome binding site of the downstream gene in the absence of theophylline and thus inhibit the translation of the gene while expose the ribosome binding site in the presence of theophylline, and the CheZ gene, which is a phosphatase which dephosphorylates CheY-P and causes the flagellum to rotate counterclockwise and is essential for keeping the motility of E.coli. Thus this part provides a ligand-inducible motility and makes it possible to guide E.coli by theophylline.
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We found the sequence of this part in literature and tried to design primers to construct it. But we were faced with a challenging problem in primer design, which comes from the basic properties of the aptamer involved. The aptamer tends to pair the ribosome binding site in the absence of theophylline, which is true in PCR, and enables primers to pair to the corresponding parts and the new strand to elongate. We made contact with the author of our reference and found they took different strategies in assembling the aptamer to CheZ instead of PCR. But thanks to the warm help of Professor J. S. Parkinson and Professor J. P. Gallivan, we acquired the sequence of primers used in assembling the aptamer to another gene and designed our own after analyzing them: forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGGGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACCCCGCTGCAAGACAACAAG ATGCAACCATCAATCAAACC-3' reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTATTATTAAAATCCAAGACTATCCAACAAATCGT-3' Besides, we used special protocal of PCR: 96℃ 10min 96℃ 30s, 56℃ 30s, 72℃ 1min for 16 cycles and each cycle the annealing temperature increases by 1℃ 96℃ 30s, 72℃ 30s, 72℃ 1min for 20 cycles 72℃ 10min
star: false
creator: Yinghong Lan
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: aptamer
sequence annotation: rbs
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K557001_sequence/1

aptamer
type: SequenceAnnotation
persistent identity: annotation2149326
display id: annotation2149326
version: 1
top level: BBa_K557001
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149326/range2149326/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K557001/annotation2149326/range2149326/1
type: Range
persistent identity: range2149326
display id: range2149326
version: 1
top level: BBa_K557001
direction: direction/forward
start: 1
end: 46
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2149327
display id: annotation2149327
version: 1
top level: BBa_K557001
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149327/range2149327/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K557001/annotation2149327/range2149327/1
type: Range
persistent identity: range2149327
display id: range2149327
version: 1
top level: BBa_K557001
direction: direction/forward
start: 47
end: 57
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2149328
display id: annotation2149328
version: 1
top level: BBa_K557001
location: https://synbiohub.org/public/igem/BBa_K557001/annotation2149328/range2149328/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K557001/annotation2149328/range2149328/1
type: Range
persistent identity: range2149328
display id: range2149328
version: 1
top level: BBa_K557001
direction: direction/forward
start: 58
end: 60
orientation: inline

BBa_K228003
type: ComponentDefinition
persistent identity: BBa K228003
display id: BBa_K228003
version: 1
was generated by: iGEM to SBOL conversion
description: Bistable part
created: 2009-09-05T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: The part is made up of the standard parts from partsregistry, and some of the sub-parts are inverted by PCR.
owned by: james
owned by: myers
mutable description: It is a part which has two stable states--CI and CI434 and the orginal state can be either of the two. CI repressor is expressed after a CI434 repressible promoter, while the CI434 is expressed after a CI repressible promoter, thus the system can only stay in one state, when one is expressed and the other is repressed. The two states can be swicthed in certain conditions. In other words, the orginal state can be changed by exogenetic triggering. For example, the orginal state is CI, then you can use an gene which exogenetically expresses CI434 to trigger the bistable part into the state of CI434.
discontinued: false
dominant: true
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4403
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Shan Shen
type: DnaRegion
role: SO:0000804
role: partType/Device
sequence annotation: mRFP
sequence annotation: rbs
sequence annotation: lamda CI repressor
sequence annotation: CI434 regulated promoter
sequence annotation: CI434 OR1
sequence annotation: CI regulated promoter
sequence annotation: CI434 repressor
sequence annotation: eGFP
sequence: https://synbiohub.org/public/igem/BBa_K228003_sequence/1

mRFP
type: SequenceAnnotation
persistent identity: annotation2028122
display id: annotation2028122
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2028122/range2028122/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228003/annotation2028122/range2028122/1
type: Range
persistent identity: range2028122
display id: range2028122
version: 1
top level: BBa_K228003
direction: direction/reverse
start: 1
end: 681
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2028127
display id: annotation2028127
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2028127/range2028127/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K228003/annotation2028127/range2028127/1
type: Range
persistent identity: range2028127
display id: range2028127
version: 1
top level: BBa_K228003
direction: direction/reverse
start: 688
end: 700
orientation: inline

lamda CI repressor
type: SequenceAnnotation
persistent identity: annotation2028126
display id: annotation2028126
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2028126/range2028126/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228003/annotation2028126/range2028126/1
type: Range
persistent identity: range2028126
display id: range2028126
version: 1
top level: BBa_K228003
direction: direction/reverse
start: 707
end: 1420
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2028128
display id: annotation2028128
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2028128/range2028128/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K228003/annotation2028128/range2028128/1
type: Range
persistent identity: range2028128
display id: range2028128
version: 1
top level: BBa_K228003
direction: direction/reverse
start: 1426
end: 1432
orientation: inline

CI434 regulated promoter
type: SequenceAnnotation
persistent identity: annotation2035764
display id: annotation2035764
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2035764/range2035764/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K228003/annotation2035764/range2035764/1
type: Range
persistent identity: range2035764
display id: range2035764
version: 1
top level: BBa_K228003
direction: direction/reverse
start: 1439
end: 1490
orientation: inline

CI434 OR1
type: SequenceAnnotation
persistent identity: annotation2035763
display id: annotation2035763
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2035763/range2035763/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K228003/annotation2035763/range2035763/1
type: Range
persistent identity: range2035763
display id: range2035763
version: 1
top level: BBa_K228003
direction: direction/reverse
start: 1439
end: 1454
orientation: inline

CI regulated promoter
type: SequenceAnnotation
persistent identity: annotation2035515
display id: annotation2035515
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2035515/range2035515/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K228003/annotation2035515/range2035515/1
type: Range
persistent identity: range2035515
display id: range2035515
version: 1
top level: BBa_K228003
direction: direction/forward
start: 1491
end: 1539
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2035614
display id: annotation2035614
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2035614/range2035614/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K228003/annotation2035614/range2035614/1
type: Range
persistent identity: range2035614
display id: range2035614
version: 1
top level: BBa_K228003
direction: direction/forward
start: 1546
end: 1554
orientation: inline

CI434 repressor
type: SequenceAnnotation
persistent identity: annotation2028124
display id: annotation2028124
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2028124/range2028124/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228003/annotation2028124/range2028124/1
type: Range
persistent identity: range2028124
display id: range2028124
version: 1
top level: BBa_K228003
direction: direction/forward
start: 1561
end: 2196
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2028125
display id: annotation2028125
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2028125/range2028125/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K228003/annotation2028125/range2028125/1
type: Range
persistent identity: range2028125
display id: range2028125
version: 1
top level: BBa_K228003
direction: direction/forward
start: 2203
end: 2211
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2028123
display id: annotation2028123
version: 1
top level: BBa_K228003
location: https://synbiohub.org/public/igem/BBa_K228003/annotation2028123/range2028123/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228003/annotation2028123/range2028123/1
type: Range
persistent identity: range2028123
display id: range2028123
version: 1
top level: BBa_K228003
direction: direction/forward
start: 2219
end: 2938
orientation: inline

https://synbiohub.org/public/igem/BBa_K557010_sequence/1
type: Sequence
persistent identity: BBa K557010 sequence
display id: BBa_K557010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtacagtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaacagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggctccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatggcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgctttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatctttgcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaagatgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttccgctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagttgcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctggtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataaaggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagtgggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaacgcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaatcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaacttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatggctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgcatttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaaaataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaaaacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaactacgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagtaaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcagggaagagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgtggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacagaggctactagagttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K117001_sequence/1
type: Sequence
persistent identity: BBa K117001 sequence
display id: BBa_K117001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatatccctccctgacttgacagagaaaataatggcgagtactttttgatctgtacataaaaccagtggttttatgtacagtattaactatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggagatggacgtggccacaacagtggcgcacataacacaggtggtaacattaatggcggccctacggggcttggtggaaatggtggggcttctgacggctccggatggagttcggaaaataacccatggggtggcggttccggtagtggtgttcactggggaggtggctccggccatggcaatggcggggggaatagtaattccggtggtggcagcaattcatccgtagcagcacctatggcatttggttttcctgctttggctgctcctggtgccggaactcttggtatttccgtatctggtgaggctttatctgcggcaatagcggatatctttgcagccctgaaaggtccgtttaaattcagcgcatggggtattgcgctttacggcattctgccatctgaaatagcaaaagatgacccgaatatgatgtcaaagattgtgacgtcattaccggcagaaacagtgacgaatgttcaggtcagtacacttccgctggaccaggcaactgtcagcgtgacgaaacgtgtgacagatgttgtgaaggacacacgacagcatattgccgtagttgcaggtgtgcctatgagtgtgcctgttgtgaatgccaaaccaacacgtactccaggtgtatttcacgcatcatttcctggtgtcccttctctgacagtcagcactgtcaaaggccttccggtgtcaacaacccttccccgtggtattacggaggataaaggccggactgccgttcctgcgggatttacctttggtggtggttcacatgaagcggtgattcgctttccgaaagaaagtgggcagaagccggtttatgtatcagtgacagatgttcttaccccagcacaggtaaaacaacgtcaggatgaggaaaaacgcctccagcaggaatggaatgacgcacatccggtggaagtggctgaacgcaattatgaacaggcccgcgcagaactgaatcaggctaataaagatgttgccagaaaccaggaacgacaggctaaagctgttcaggtttataattctcgtaaaagtgaacttgatgcagcgaataaaactcttgctgatgcaaaggctgaaataaaacaattcgagcgatttgcccgagaaccaatggctgctggtcacagaatgtggcaaatggcagggcttaaggcccagcgggcacagacggatgtaaataataagaaggctgcatttgatgctgctgcaaaagaaaagtcagatgctgatgttgcattgagttctgcgttggagcgccgcaaacagaaagaaaataaagaaaaggacgctaaagccaaattagataaggagagtaaacggaataagccagggaaggcaacaggtaaaggaaaacctgtcaataataagtggttaaataatgcaggtaaagacttaggttctcctgttccagatcgtatagctaataaactacgtgataaggagtttaaaagtttcgatgattttcgtaagaaattctgggaagaagtgtcaaaagatcctgagttaagtaaacaatttagtaggaacaataatgatcgaatgaaggttggaaaagcgcccaagactagaacccaggatgtttcagggaagagaacttcattcgagcttcatcatgagaagccgatcagccaaaatggtggtgtctatgatatggataacatcagcgtggtaacacctaaaaggcatattgatattcaccgaggtaaataatatggaactgaaaaatagtattagtgattacacagaggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K557009_sequence/1
type: Sequence
persistent identity: BBa K557009 sequence
display id: BBa_K557009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K117000_sequence/1
type: Sequence
persistent identity: BBa K117000 sequence
display id: BBa_K117000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaataacagggattattttattgcttcttgcagccattattcttgctgcatgtcaggcaaactatatccgtgatgttcagggcgggacagtatcaccgtcgtcaactgctgaactgaccggagtggaaacgcagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K557002_sequence/1
type: Sequence
persistent identity: BBa K557002 sequence
display id: BBa_K557002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K557001_sequence/1
type: Sequence
persistent identity: BBa K557001 sequence
display id: BBa_K557001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagacttggattttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228003_sequence/1
type: Sequence
persistent identity: BBa K228003 sequence
display id: BBa_K228003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttcctcctactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactagatactaaggaggttgtatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactgg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z