LOCUS       BBa_K558001             3047 bp    DNA     linear       22-Nov-2024
DEFINITION  s70/PDC/ADH Ethanol Generator
ACCESSION   BBa_K558001
VERSION     BBa_K558001.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=Part:BBa_J23101 
     RBS             37..51
                     /label=RBS
     CDS             52..1757
                     /label=PDC Coding Region
     misc_feature    1756..1761
                     /label=2x Stop
     misc_feature    1764..1771
                     /label=NotI Cut Site
     misc_feature    2952..3047
                     /label=BBa_B0014
     misc_feature    1772..1778
                     /label=XhoI Cut Site
     misc_feature    2941..2952
                     /label=4x Stop
     misc_feature    52..54
                     /label=start
     misc_feature    1762..1791
                     /label=Stuffer Region
     CDS             1792..2940
                     /label=ADH Coding Region
     misc_feature    1792..1794
                     /label=start
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagcataaa ggaggtaaat aatgagttat
       61 actgtcggta cctatttagc ggagcggctt gtccagattg gtctcaagca tcacttcgca
      121 gtcgcgggcg actacaacct cgtccttctt gacaacctgc ttttgaacaa aaacatggag
      181 caggtttatt gctgtaacga actgaactgc ggtttcagtg cagaaggtta tgctcgtgcc
      241 aaaggcgcag cagcagccgt cgttacctac agcgtcggtg cgctttccgc atttgatgct
      301 atcggtggcg cctatgcaga aaaccttccg gttatcctga tctccggtgc tccgaacaac
      361 aatgatcacg ctgctggtca cgtgttgcat cacgctcttg gcaaaaccga ctatcactat
      421 cagttggaaa tggccaagaa catcacggcc gcagctgaag cgatttacac cccagaagaa
      481 gctccggcta aaatcgatca cgtgattaaa actgctcttc gtgagaagaa gccggtttat
      541 ctcgaaatcg cttgcaacat tgcttccatg ccctgcgccg ctcctggacc ggcaagcgca
      601 ttgttcaatg acgaagccag cgacgaagct tctttgaatg cagcggttga agaaaccctg
      661 aaattcatcg ccaaccgcga caaagttgcc gtcctcgtcg gcagcaagct gcgcgcagct
      721 ggtgctgaag aagctgctgt caaatttgct gatgctctcg gtggcgcagt tgctaccatg
      781 gctgctgcaa aaagcttctt cccagaagaa aacccgcatt acatcggtac ctcatggggt
      841 gaagtcagct atccgggcgt tgaaaagacg atgaaagaag ccgatgcggt tatcgctctg
      901 gctcctgtct tcaacgacta ctccaccact ggttggacgg atattcctga tcctaagaaa
      961 ctggttctcg ctgaaccgcg ttctgtcgtc gttaacggcg ttcgcttccc cagcgttcat
     1021 ctgaaagact atctgacccg tttggctcag aaagtttcca agaaaaccgg tgctttggac
     1081 ttcttcaaat ccctcaatgc aggtgaactg aagaaagccg ctccggctga tccgagtgct
     1141 ccgttggtca acgcagaaat cgcccgtcag gtcgaagctc ttctgacccc gaacacgacg
     1201 gttattgctg aaaccggtga ctcttggttc aatgctcagc gcatgaagct cccgaacggt
     1261 gctcgcgttg aatatgaaat gcagtggggt cacatcggtt ggtccgttcc tgccgccttc
     1321 ggttatgccg tcggtgctcc ggaacgtcgc aacatcctca tggttggtga tggttccttc
     1381 cagctgacgg ctcaggaagt cgctcagatg gttcgcctga aactgccggt tatcatcttc
     1441 ttgatcaata actatggtta caccatcgaa gttatgatcc atgatggtcc gtacaacaac
     1501 atcaagaact gggattatgc cggtctgatg gaagtgttca acggtaacgg tggttatgac
     1561 agcggtgctg gtaaaggcct gaaggctaaa accggtggcg aactggcaga agctatcaag
     1621 gttgctctgg caaacaccga cggcccaacc ctgatcgaat gcttcatcgg tcgtgaagac
     1681 tgcactgaag aattggtcaa atggggtaag cgcgttgctg ccgccaacag ccgtaagcct
     1741 gttaacaagc tcctctaata agcggccgcc tcgagataaa ggaggtaaat aatggcttcc
     1801 tcaacttttt atattccttt cgtcaacgaa atgggggaag gttcgcttga aaaagcaatc
     1861 aaggatctta acggcagcgg ctttaaaaat gcgctgatcg tttctgatgc tttcatgaac
     1921 aaatccggtg ttgtgaagca ggttgctgac ctgttgaaag cacagggtat taattctgct
     1981 gtttatgatg gcgttatgcc gaacccgact gttaccgcag ttctggaagg ccttaagatc
     2041 ctgaaggata acaattcaga tttcgtcatc tccctcggtg gtggttctcc ccatgactgc
     2101 gccaaggcca tcgctctggt cgcaaccaat ggtggtgaag tcaaagacta cgaaggtatc
     2161 gacaaatcta agaaacctgc cctgcctttg atgtcaatca acacgacggc tggtacggct
     2221 tctgaaatga cgcgtttctg catcatcact gatgaagtcc gtcacgttaa gatggccatt
     2281 gttgaccgtc acgttacccc gatggtttcc gtcaacgatc ctctgttgat ggttggtatg
     2341 ccaaaaggcc tgaccgccgc caccggtatg gatgctctga cccacgcatt tgaagcctat
     2401 tcttcaacgg cagctactcc gatcaccgat gcttgcgcct tgaaggctgc gtccatgatc
     2461 gctaagaatc tgaagaccgc ttgcgacaac ggtaaggata tgccagctcg tgaagctatg
     2521 gcttatgccc aattcctcgc tggtatggcc ttcaacaacg cttcgcttgg ttatgtccat
     2581 gctatggctc accagttggg cggctactac aacctgccgc atggtgtctg caacgctgtt
     2641 ctgcttccgc atgttctggc ttataacgcc tctgtcgttg ctggtcgtct gaaagacgtt
     2701 ggtgttgcta tgggtctcga tatcgccaat ctcggtgata aagaaggcgc agaagccacc
     2761 attcaggctg ttcgcgatct agctgcttcc attggtattc cagcaaatct gaccgagctg
     2821 ggtgctaaga aagaagatgt gccgcttctt gctgaccacg ctctgaaaga tgcttgtgct
     2881 ctgaccaacc cgcgtcaggg tgatcagaaa gaagttgaag aactcttcct gagcgctttc
     2941 taataataat aatcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     3001 gagagaatat aaaaagccag attattaatc cggctttttt attattt
//