BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_K559010
1
BBa_K559010
Halorhodopsin complete system
2011-09-28T11:00:00Z
2015-05-08T01:12:41Z
Halorhodopsin comes from part BBa_K559000, which is come from genomic sequence of N.pharaonis (genebank ID:J05199.1)
Halorhodopsin gene allow chloride ion to be pumped in by the level of light intensity. Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized, and the bidirectional terminator is added for high efficiency termination of both forward and backward transcription
false
false
_727_
0
6123
9
It's complicated
true
The double terminator wass tested to stop effectively on the transcription of both forward and backward transcription to make our protein generator be a controlled system
false
Jacky, Fong Chuen, Loo
component2177581
1
BBa_B0014
component2177574
1
BBa_K559001
annotation2177581
1
BBa_B0014
range2177581
1
737
831
annotation2177574
1
BBa_K559001
range2177574
1
1
728
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K559001
1
BBa_K559001
Halorhodopsin under T7 promoter
2011-09-28T11:00:00Z
2015-05-08T01:12:41Z
Halorhodopsin come from N.pharaonis, gene bank J05199.1
Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor
false
false
_727_
0
6123
9
It's complicated
false
The coding sequence of N.pharaonis J05199.1 is selected from 61nt to 700nt and the T7 promotor placed in front by 3A assembly
false
Jacky, Fong Chuen, Loo
annotation2177551
1
T7 promotor
range2177551
1
1
16
annotation2177555
1
RBS
range2177555
1
66
77
annotation2177554
1
Halorhodopsin
range2177554
1
89
728
annotation2177553
1
lac repressor
range2177553
1
22
42
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K559001_sequence
1
taatacgactcactataggggaattgtgagcggataacaattccccaataattttgtttaactttaagaagaagatatagtactagagatgactgagacattgccaccggtaacggaatcggctgttgcgctacaggcggaggtgacccagagggagctgttcgagttcgttctcaacgaccccctcctcgccagttcgctgtatattaatatcgcactggcagggctgtcgatactgcttttcgtgttcatgacgcgcggactcgacgacccacgggcgaaactcatcgccgtttcgacgattttggtgccggtggtctctatcgcgagctacaccggccttgcatcggggctcaccatcagcgtcctcgagatgccagccggccacttcgccgaggggtcctcggtgatgctcggcggcgaagaggtagacggcgtcgtgacgatgtggggccgctatctgacgtgggccctttcgacaccgatgatactgctggcgcttgggctgcttgctggctctaacgccacgaagctctttaccgccatcaccttcgacatcgcgatgtgtgtcaccggcctcgcagccgcgctgacgacctcttcgcacctgatgcggtggttctggtacgccatcagttgtgcgtgtttcctcgtcgtcctctacatcctgctcgtcgagtgggcacaggacgccaaggctgccggtactgcggatatgttcaatacgc
BBa_K559010_sequence
1
taatacgactcactataggggaattgtgagcggataacaattccccaataattttgtttaactttaagaagaagatatagtactagagatgactgagacattgccaccggtaacggaatcggctgttgcgctacaggcggaggtgacccagagggagctgttcgagttcgttctcaacgaccccctcctcgccagttcgctgtatattaatatcgcactggcagggctgtcgatactgcttttcgtgttcatgacgcgcggactcgacgacccacgggcgaaactcatcgccgtttcgacgattttggtgccggtggtctctatcgcgagctacaccggccttgcatcggggctcaccatcagcgtcctcgagatgccagccggccacttcgccgaggggtcctcggtgatgctcggcggcgaagaggtagacggcgtcgtgacgatgtggggccgctatctgacgtgggccctttcgacaccgatgatactgctggcgcttgggctgcttgctggctctaacgccacgaagctctttaccgccatcaccttcgacatcgcgatgtgtgtcaccggcctcgcagccgcgctgacgacctcttcgcacctgatgcggtggttctggtacgccatcagttgtgcgtgtttcctcgtcgtcctctacatcctgctcgtcgagtgggcacaggacgccaaggctgccggtactgcggatatgttcaatacgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z