LOCUS       BBa_K560000             1676 bp    DNA     linear       22-Nov-2024
DEFINITION  Blue Light Sensor+PtrpL+mRFP1
ACCESSION   BBa_K560000
VERSION     BBa_K560000.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23117
     RBS             44..56
                     /label=RBS B0032
     misc_feature    745..749
                     /label=stop codon
     CDS             61..744
                     /label=LovTAP K360022
     stem_loop       768..814
                     /label=Terminator B0010
     stem_loop       847..873
                     /label=Terminator B0012
     polyA_site      874..887
                     /label=AAA
     misc_feature    44..887
                     /label=BBa_K360121
     misc_feature    924..940
                     /label=trpR binding site -3.5
     misc_feature    916..935
                     /label=trpR binding site  -11.5
     misc_feature    908..925
                     /label=trpR binding site  -19.5
     promoter        898..906
                     /label=-35 
     promoter        923..930
                     /label=-10
     promoter        896..944
                     /label=BBa_K360023
     misc_feature    957..960
                     /label=conserved
     RBS             953..964
                     /label=BBa_B0034
     CDS             971..1645
                     /label=mrfp1
     misc_feature    1652..1676
                     /label=Help:Barcodes
     CDS             971..1676
                     /label=BBa_E1010
ORIGIN
        1 ttgacagcta gctcagtcct agggattgtg ctagctacta gagtcacaca ggaaagtact
       61 atgttggcta ctacacttga acgtattgag aagaactttg tcattactga cccaaggttg
      121 ccagataatc cctttatatt cgcgtccgat agtttcttgc agttgacaga atatagccgt
      181 gaagaaattt tgggaagaaa ttgtcgtttt ctacaaggtc ctgaaactga tcgcgcgaca
      241 gtgagaaaaa ttagagatgc catagataac caaacagagg tcactgttca gctgattaat
      301 tatacaaaga gtggtaaaaa gttctggaac ctctttcact tgcagcctat gcgagatcag
      361 aagggagatg tccagtactt tattggggtt cagttggatg gaactgagca tgtccgagat
      421 gctgccgaga gagagggagt catgctgatt aagaaaaccg ccgaaaatat tgatgaggcg
      481 gcatttgtcg acctgcttaa gaatgcctac caaaacgatc tccatttacc gttgttaaac
      541 ctgatgctga cgccagatga gcgcgaagcg ttggggactc gcgtgcgtat tgtcgaagag
      601 ctgttgcgcg gcgaaatgag ccagcgtgag ttaaaaaatg aactcggcgc gggcatcgcg
      661 acgattacgc gtggatctaa cagcctgaaa gccgcgcccg tggagctgcg ccagtggctg
      721 gaagaggtgt tgctgaaaag cgattaataa tactagagcc aggcatcaaa taaaacgaaa
      781 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      841 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagaggctgt
      901 tgacaattaa tcatcgaact agttaactag tacgcaagtt cacgtactag agaaagagga
      961 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
     1021 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
     1081 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
     1141 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
     1201 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
     1261 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
     1321 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
     1381 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1441 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1501 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1561 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1621 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgc
//