LOCUS BBa_K560016 3007 bp DNA linear 23-Dec-2024 DEFINITION BsaI Golden Gate Assembly_Backbone ACCESSION BBa_K560016 VERSION BBa_K560016.1 FEATURES Location/Qualifiers misc_feature 575..580 /label=BsaI misc_feature 128..133 /label=BsaI CDS 154..309 /label=LacZ-α CDS 784..1794 /label=spect misc_feature 1871..2480 /label=pBR322_origin ORIGIN 1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac 61 gacgttgtaa aacgacggcc agtcttaagc gtctcatggc ctgaccccgg accaagtggt 121 ggctatcgag accggcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact 181 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 241 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 301 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc 361 ccctcgaggt cctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa 421 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 481 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 541 attgcgttgc gctcactgcc cgctttccac cggtggtctc ctgttgccgg tgctgtgcca 601 ggaccatggc ctgaccccgg accaagtggt ggctatcgag acgtctagac cagccaggac 661 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg 721 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag 781 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca 841 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca 901 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat 961 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc 1021 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg 1081 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt 1141 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg 1201 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta 1261 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa 1321 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg 1381 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta 1441 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta 1501 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta 1561 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat 1621 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa 1681 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg 1741 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg 1801 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac 1861 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1921 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1981 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 2041 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 2101 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 2161 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 2221 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 2281 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2341 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2401 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2461 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 2521 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 2581 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 2641 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 2701 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 2761 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 2821 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 2881 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 2941 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 3001 gttttat //