LOCUS       BBa_K561000             1733 bp    DNA     linear       22-Nov-2024
DEFINITION  Pvgb+YFP+tetR
ACCESSION   BBa_K561000
VERSION     BBa_K561000.1  
FEATURES             Location/Qualifiers
     promoter        1..135
                     /label=vgb promoter
     promoter        1..135
                     /label=BBa_K561001
     RBS             144..156
                     /label=RBS-3\Weak
     RBS             150..153
                     /label=RBS
     misc_feature    144..156
                     /label=BBa_B0032
     RBS             144..156
                     /label=BBa_B0032
     CDS             163..885
                     /label=BBa_E0030
     misc_feature    144..885
                     /label=BBa_I15017
     misc_feature    898..901
                     /label=conserved
     RBS             894..905
                     /label=BBa_B0034
     CDS             915..1531
                     /label=tetR
     misc_feature    1532..1565
                     /label=SsrA
     misc_feature    1572..1596
                     /label=Help:Barcodes
     CDS             912..1571
                     /label=BBa_C0040
     stem_loop       1616..1659
                     /label=stem_loop
     misc_feature    1605..1684
                     /label=BBa_B0010
     terminator      1605..1684
                     /label=BBa_B0010
     stem_loop       1700..1719
                     /label=T7 TE
     misc_feature    1726..1726
                     /label=stop
     polyA_site      1720..1733
                     /label=polya
     misc_feature    1693..1733
                     /label=BBa_B0012
     terminator      1693..1733
                     /label=BBa_B0012
     misc_feature    894..1733
                     /label=BBa_P0440
ORIGIN
        1 acaggacgct ggggttaaaa gtatttgagt tttgatgtgg attaagtttt aagaggcaat
       61 aaagattata ataagtgctg ctacaccata ctgatgtatg gcaaaaccat aataatgaac
      121 ttaaggaaga ccctctacta gagtcacaca ggaaagtact agatggtgag caagggcgag
      181 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      241 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      301 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
      361 tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga cttcttcaag
      421 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      481 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      541 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      601 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      661 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      721 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
      781 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      841 gccgccggga tcactctcgg catggacgag ctgtacaagt aataatacta gagaaagagg
      901 agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc
      961 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag
     1021 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca
     1081 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag
     1141 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag
     1201 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat
     1261 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg
     1321 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag
     1381 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat
     1441 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat
     1501 tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag
     1561 tagcttaata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa
     1621 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1681 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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