##gff-version 3
##sequence-region BBa_K561000 1 1733
BBa_K561000	.	engineered_region	144	885	.	+	0	ID=BBa_I15017;Name=BBa_I15017
BBa_K561000	.	CDS	163	885	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I15017
BBa_K561000	.	ribosome_entry_site	144	156	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I15017
BBa_K561000	.	ribosome_entry_site	144	156	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K561000	.	engineered_region	144	156	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K561000	.	ribosome_entry_site	150	153	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K561000	.	promoter	1	135	.	+	0	ID=BBa_K561001;Name=BBa_K561001
BBa_K561000	.	promoter	1	135	.	+	0	ID=annotation2146939;Name=vgb promoter;Parent=BBa_K561001
BBa_K561000	.	engineered_region	894	1733	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K561000	.	terminator	1693	1733	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K561000	.	stop_codon	1726	1726	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K561000	.	polyA_site	1720	1733	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K561000	.	stem_loop	1700	1719	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K561000	.	engineered_region	1693	1733	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K561000	.	terminator	1605	1684	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K561000	.	engineered_region	1605	1684	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K561000	.	stem_loop	1616	1659	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K561000	.	CDS	912	1571	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K561000	.	engineered_tag	1572	1596	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K561000	.	CDS	915	1531	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K561000	.	tag	1532	1565	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K561000	.	ribosome_entry_site	894	905	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K561000	.	conserved_region	898	901	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K561000
acaggacgctggggttaaaagtatttgagttttgatgtggattaagttttaagaggcaataaagattataataagtgct
gctacaccatactgatgtatggcaaaaccataataatgaacttaaggaagaccctctactagagtcacacaggaaagta
ctagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggc
cacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccg
gcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgacca
catgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgac
ggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgact
tcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaa
gcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactac
cagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagca
aagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacga
gctgtacaagtaataatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcg
cattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcc
tacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatact
cacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaa
gtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagc
ctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgc
gtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattac
gacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatt
agaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatag
tgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata