LOCUS       BBa_K561002             1679 bp    DNA     linear       25-Nov-2024
DEFINITION  PfdhF+RFP+tetR
ACCESSION   BBa_K561002
VERSION     BBa_K561002.1  
FEATURES             Location/Qualifiers
     promoter        1..99
                     /label=BBa_K387003
     misc_feature    112..115
                     /label=conserved
     RBS             108..119
                     /label=BBa_B0034
     CDS             126..800
                     /label=mrfp1
     misc_feature    807..831
                     /label=Help:Barcodes
     CDS             126..831
                     /label=BBa_E1010
     misc_feature    108..831
                     /label=BBa_K093005
     misc_feature    844..847
                     /label=conserved
     RBS             840..851
                     /label=BBa_B0034
     CDS             861..1477
                     /label=tetR
     misc_feature    1478..1511
                     /label=SsrA
     misc_feature    1518..1542
                     /label=Help:Barcodes
     CDS             858..1517
                     /label=BBa_C0040
     stem_loop       1562..1605
                     /label=stem_loop
     misc_feature    1551..1630
                     /label=BBa_B0010
     terminator      1551..1630
                     /label=BBa_B0010
     stem_loop       1646..1665
                     /label=T7 TE
     misc_feature    1672..1672
                     /label=stop
     polyA_site      1666..1679
                     /label=polya
     misc_feature    1639..1679
                     /label=BBa_B0012
     terminator      1639..1679
                     /label=BBa_B0012
     misc_feature    840..1679
                     /label=BBa_P0440
ORIGIN
        1 tgaataaata gtgcccgtaa tatcagggaa tgaccccaca taaaatgtgg cataaaagat
       61 gcatactgta gtcgagagcg cgtatgcgtg atttgattat actagagaaa gaggagaaat
      121 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
      181 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
      241 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
      301 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
      361 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
      421 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
      481 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
      541 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
      601 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
      661 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
      721 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
      781 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagaga
      841 aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac agcgcattag
      901 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
      961 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
     1021 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
     1081 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
     1141 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
     1201 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
     1261 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
     1321 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
     1381 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
     1441 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg
     1501 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca
     1561 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1621 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//