LOCUS BBa_K561003 4407 bp DNA linear 22-Dec-2024 DEFINITION PfdhF+RFP+tetR+Pvgb+YFP+tetR+Ptet+CFP ACCESSION BBa_K561003 VERSION BBa_K561003.1 FEATURES Location/Qualifiers promoter 1..99 /label=BBa_K387003 misc_feature 112..115 /label=conserved RBS 108..119 /label=BBa_B0034 CDS 126..800 /label=mrfp1 misc_feature 807..831 /label=Help:Barcodes CDS 126..831 /label=BBa_E1010 misc_feature 108..831 /label=BBa_K093005 misc_feature 844..847 /label=conserved RBS 840..851 /label=BBa_B0034 CDS 861..1477 /label=tetR misc_feature 1478..1511 /label=SsrA misc_feature 1518..1542 /label=Help:Barcodes CDS 858..1517 /label=BBa_C0040 stem_loop 1562..1605 /label=stem_loop misc_feature 1551..1630 /label=BBa_B0010 terminator 1551..1630 /label=BBa_B0010 stem_loop 1646..1665 /label=T7 TE misc_feature 1672..1672 /label=stop polyA_site 1666..1679 /label=polya misc_feature 1639..1679 /label=BBa_B0012 terminator 1639..1679 /label=BBa_B0012 misc_feature 840..1679 /label=BBa_P0440 misc_feature 1..1679 /label=BBa_K561002 promoter 1688..1822 /label=vgb promoter promoter 1688..1822 /label=BBa_K561001 RBS 1831..1843 /label=RBS-3\Weak RBS 1837..1840 /label=RBS misc_feature 1831..1843 /label=BBa_B0032 RBS 1831..1843 /label=BBa_B0032 CDS 1850..2572 /label=BBa_E0030 misc_feature 1831..2572 /label=BBa_I15017 misc_feature 2585..2588 /label=conserved RBS 2581..2592 /label=BBa_B0034 CDS 2602..3218 /label=tetR misc_feature 3219..3252 /label=SsrA misc_feature 3259..3283 /label=Help:Barcodes CDS 2599..3258 /label=BBa_C0040 stem_loop 3303..3346 /label=stem_loop misc_feature 3292..3371 /label=BBa_B0010 terminator 3292..3371 /label=BBa_B0010 stem_loop 3387..3406 /label=T7 TE misc_feature 3413..3413 /label=stop polyA_site 3407..3420 /label=polya misc_feature 3380..3420 /label=BBa_B0012 terminator 3380..3420 /label=BBa_B0012 misc_feature 2581..3420 /label=BBa_P0440 misc_feature 1688..3420 /label=BBa_K561000 misc_feature 3429..3447 /label=TetR 1 misc_feature 3429..3482 /label=BBa_R0040 promoter 3448..3453 /label=-35 misc_feature 3454..3472 /label=TetR 2 promoter 3471..3476 /label=-10 promoter 3429..3482 /label=BBa_R0040 misc_feature 3495..3498 /label=conserved RBS 3491..3502 /label=BBa_B0034 CDS 3509..4270 /label=CFP (LVA) misc_feature 4227..4264 /label=SsrA misc_feature 4265..4270 /label=2 misc_feature 3577..3577 /label=A misc_feature 3509..4270 /label=BBa_E0022 misc_feature 3509..4270 /label=BBa_E0022 stem_loop 4290..4333 /label=stem_loop misc_feature 4279..4358 /label=BBa_B0010 terminator 4279..4358 /label=BBa_B0010 stem_loop 4374..4393 /label=T7 TE misc_feature 4400..4400 /label=stop polyA_site 4394..4407 /label=polya misc_feature 4367..4407 /label=BBa_B0012 terminator 4367..4407 /label=BBa_B0012 misc_feature 3429..4407 /label=BBa_I13602 ORIGIN 1 tgaataaata gtgcccgtaa tatcagggaa tgaccccaca taaaatgtgg cataaaagat 61 gcatactgta gtcgagagcg cgtatgcgtg atttgattat actagagaaa gaggagaaat 121 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 181 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 241 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 301 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 361 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 421 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 481 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 541 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 601 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 661 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 721 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 781 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagaga 841 aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac agcgcattag 901 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag 961 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct 1021 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct 1081 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg 1141 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa 1201 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca 1261 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta 1321 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg 1381 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat 1441 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg 1501 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca 1561 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1621 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 1681 actagagaca ggacgctggg gttaaaagta tttgagtttt gatgtggatt aagttttaag 1741 aggcaataaa gattataata agtgctgcta caccatactg atgtatggca aaaccataat 1801 aatgaactta aggaagaccc tctactagag tcacacagga aagtactaga tggtgagcaa 1861 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 1921 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 1981 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 2041 cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt 2101 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 2161 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 2221 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 2281 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 2341 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 2401 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta 2461 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 2521 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat aatactagag 2581 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 2641 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 2701 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 2761 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 2821 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 2881 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 2941 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 3001 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 3061 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 3121 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 3181 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 3241 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 3301 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3361 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 3421 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc 3481 actactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg 3541 ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc 3601 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 3661 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg 3721 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 3781 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 3841 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 3901 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt 3961 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa 4021 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 4081 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 4141 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 4201 tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt 4261 agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 4321 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 4381 cttcgggtgg gcctttctgc gtttata //