##gff-version 3
##sequence-region BBa_K561003 1 4407
BBa_K561003	.	engineered_region	1688	3420	.	+	0	ID=BBa_K561000;Name=BBa_K561000
BBa_K561003	.	engineered_region	1831	2572	.	+	0	ID=BBa_I15017;Name=BBa_I15017;Parent=BBa_K561000
BBa_K561003	.	ribosome_entry_site	1831	1843	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I15017
BBa_K561003	.	ribosome_entry_site	1837	1840	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K561003	.	ribosome_entry_site	1831	1843	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K561003	.	engineered_region	1831	1843	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K561003	.	CDS	1850	2572	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I15017
BBa_K561003	.	engineered_region	2581	3420	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K561000
BBa_K561003	.	terminator	3292	3371	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K561003	.	engineered_region	3292	3371	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K561003	.	stem_loop	3303	3346	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K561003	.	terminator	3380	3420	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K561003	.	stem_loop	3387	3406	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K561003	.	stop_codon	3413	3413	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K561003	.	engineered_region	3380	3420	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K561003	.	polyA_site	3407	3420	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K561003	.	CDS	2599	3258	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K561003	.	tag	3219	3252	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K561003	.	engineered_tag	3259	3283	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K561003	.	CDS	2602	3218	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K561003	.	ribosome_entry_site	2581	2592	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K561003	.	conserved_region	2585	2588	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K561003	.	promoter	1688	1822	.	+	0	ID=BBa_K561001;Name=BBa_K561001;Parent=BBa_K561000
BBa_K561003	.	promoter	1688	1822	.	+	0	ID=annotation2146939;Name=vgb promoter;Parent=BBa_K561001
BBa_K561003	.	engineered_region	1	1679	.	+	0	ID=BBa_K561002;Name=BBa_K561002
BBa_K561003	.	engineered_region	108	831	.	+	0	ID=BBa_K093005;Name=BBa_K093005;Parent=BBa_K561002
BBa_K561003	.	ribosome_entry_site	108	119	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K093005
BBa_K561003	.	conserved_region	112	115	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K561003	.	CDS	126	831	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K093005
BBa_K561003	.	engineered_tag	807	831	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K561003	.	CDS	126	800	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K561003	.	engineered_region	840	1679	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K561002
BBa_K561003	.	terminator	1551	1630	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K561003	.	engineered_region	1551	1630	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K561003	.	stem_loop	1562	1605	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K561003	.	terminator	1639	1679	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K561003	.	stem_loop	1646	1665	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K561003	.	stop_codon	1672	1672	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K561003	.	engineered_region	1639	1679	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K561003	.	polyA_site	1666	1679	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K561003	.	CDS	858	1517	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K561003	.	tag	1478	1511	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K561003	.	engineered_tag	1518	1542	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K561003	.	CDS	861	1477	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K561003	.	ribosome_entry_site	840	851	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K561003	.	conserved_region	844	847	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K561003	.	promoter	1	99	.	+	0	ID=BBa_K387003;Name=BBa_K387003;Parent=BBa_K561002
BBa_K561003	.	engineered_region	3429	4407	.	+	0	ID=BBa_I13602;Name=BBa_I13602
BBa_K561003	.	promoter	3429	3482	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13602
BBa_K561003	.	promoter	3471	3476	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K561003	.	non_covalent_binding_site	3454	3472	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K561003	.	non_covalent_binding_site	3429	3447	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K561003	.	promoter	3448	3453	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K561003	.	engineered_region	3429	3482	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K561003	.	terminator	4279	4358	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13602
BBa_K561003	.	engineered_region	4279	4358	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K561003	.	stem_loop	4290	4333	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K561003	.	ribosome_entry_site	3491	3502	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13602
BBa_K561003	.	conserved_region	3495	3498	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K561003	.	terminator	4367	4407	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13602
BBa_K561003	.	stem_loop	4374	4393	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K561003	.	stop_codon	4400	4400	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K561003	.	engineered_region	4367	4407	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K561003	.	polyA_site	4394	4407	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K561003	.	engineered_region	3509	4270	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_I13602
BBa_K561003	.	stop_codon	4265	4270	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K561003	.	CDS	3509	4270	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K561003	.	point_mutation	3577	3577	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K561003	.	engineered_region	3509	4270	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K561003	.	tag	4227	4264	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
>BBa_K561003
tgaataaatagtgcccgtaatatcagggaatgaccccacataaaatgtggcataaaagatgcatactgtagtcgagagc
gcgtatgcgtgatttgattatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttc
atgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgt
acgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgca
gttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggt
ttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtg
agttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttg
ggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagac
ggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccg
acatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactc
caccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgtccagatta
gataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcg
cccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccat
tgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagt
atgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgc
tgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctact
actgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcg
gccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgc
tttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagacaggacgctggggttaaaagtatttgagttttgatgtggattaagtttta
agaggcaataaagattataataagtgctgctacaccatactgatgtatggcaaaaccataataatgaacttaaggaaga
ccctctactagagtcacacaggaaagtactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcct
ggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaag
ctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcc
tgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtcca
ggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtg
aaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaaca
gccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgagga
cggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccac
tacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccg
ccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagaaagaggagaaatactagatgtccaga
ttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaac
tcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagc
cattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataac
gctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaac
agtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcag
cgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacct
actactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttat
tcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaacta
cgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatact
gagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcct
ggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaag
ctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcg
tgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtcca
ggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtg
aaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatca
gccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgagga
cggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccac
tacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccg
ccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagctta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata