##gff-version 3
##sequence-region BBa_K561004 1 3813
BBa_K561004	.	ribosome_entry_site	1595	1606	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K561004	.	conserved_region	1599	1602	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K561004	.	CDS	126	1586	.	+	0	ID=BBa_K118022;Name=BBa_K118022
BBa_K561004	.	CDS	126	1583	.	+	0	ID=annotation1996993;Name=cex coding sequence;Parent=BBa_K118022
BBa_K561004	.	point_mutation	906	911	.	+	0	ID=annotation1996994;Name=PstI site removed;Parent=BBa_K118022
BBa_K561004	.	CDS	1613	2965	.	+	0	ID=BBa_K118023;Name=BBa_K118023
BBa_K561004	.	CDS	1613	2962	.	+	0	ID=annotation1996991;Name=cenA coding sequence;Parent=BBa_K118023
BBa_K561004	.	promoter	1	99	.	+	0	ID=BBa_K387003;Name=BBa_K387003
BBa_K561004	.	engineered_region	2974	3813	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K561004	.	CDS	2992	3651	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K561004	.	tag	3612	3645	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K561004	.	engineered_tag	3652	3676	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K561004	.	CDS	2995	3611	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K561004	.	terminator	3685	3764	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K561004	.	stem_loop	3696	3739	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K561004	.	engineered_region	3685	3764	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K561004	.	ribosome_entry_site	2974	2985	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K561004	.	conserved_region	2978	2981	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K561004	.	terminator	3773	3813	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K561004	.	polyA_site	3800	3813	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K561004	.	engineered_region	3773	3813	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K561004	.	stem_loop	3780	3799	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K561004	.	stop_codon	3806	3806	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K561004	.	ribosome_entry_site	108	119	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K561004	.	conserved_region	112	115	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K561004
tgaataaatagtgcccgtaatatcagggaatgaccccacataaaatgtggcataaaagatgcatactgtagtcgagagc
gcgtatgcgtgatttgattatactagagaaagaggagaaatactagatgcctaggaccacgcccgcacccggccacccg
gcccgcggcgcccgcaccgctctgcgcacgacgctcgccgccgcggcggcgacgctcgtcgtcggcgccacggtcgtgc
tgcccgcccaggccgcgaccacgctcaaggaggccgccgacggcgccggccgggacttcggcttcgcgctcgaccccaa
ccggctctcggaggcgcagtacaaggcgatcgccgacagcgagttcaacctcgtcgtcgccgagaacgcgatgaagtgg
gacgccaccgagccctcgcagaacagcttctccttcggcgcgggcgaccgcgtcgcgagctacgccgccgacaccggca
aggagctgtacggccacacgctcgtatggcactcgcagctgcccgactgggcgaagaacctcaacggctccgcgttcga
gagcgcgatggtcaaccacgtgacgaaggtcgccgaccacttcgagggcaaggtcgcgtcgtgggacgtcgtcaacgag
gcgttcgccgacggcggcggccgccggcaggactcggcgttccagcagaagctcggcaacggctacatcgagaccgcgt
tccgggcggcacgtgcggcggacccgaccgccaagctgtgcatcaacgactacaacgtcgagggcatcaacgcgaagag
caactcgctctacgacctcgtcaaggacttcaaggcgcgcggcgtcccgctcgactgcgtcgggttccagtcgcacctc
atcgtcggccaggtgccgggcgacttccggcagaacctgcaacggttcgcggacctgggcgtggacgtgcgcatcaccg
agctcgacatccgcatgcggacgccctccgacgcgaccaagctcgcgacccaggcggccgactacaagaaggtcgtgca
ggcctgcatgcaggtgacccgctgccagggcgtgaccgtctggggcatcaccgacaagtactcgtgggtgccggacgtc
ttcccgggcgagggggccgcgctggtgtgggacgcgagctacgccaagaagccggcctacgccgccgtgatggaggcct
tcggcgcgagcccgacgccgacgcccaccacgccgaccccgacgcccacgacgccgacgccgaccccgacgtccggtcc
ggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcc
tccgctccggtcgacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacgg
tcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagtt
cggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggc
taataatactagagaaagaggagaaatactagatgtccacccgcagaaccgccgcagcgctgctggcggccgcggccgt
cgccgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccggctgccgcgtcgactacgccgtcacc
aaccagtggcccggcggcttcggcgccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagctcgact
ggacctacaccgcaggccagcggatccagcagctgtggaacggcaccgcgtcgaccaacggcggccaggtctccgtcac
cagcctgccctggaacggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtgggccgggtccaac
ccgacgccggcgtcgttctcgctcaacggcaccacctgcacgggcaccgtgccgacgaccagccccacgccgaccccga
cgccgacgacccccacgccgacgccgaccccgacccccacccccacgccgacggtcacgccgcagccgaccagcggctt
ctacgtcgacccgacgacgcagggctaccgcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatc
gcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgcacgcgcaggccgaggtcgccgactacaccg
gccgcgccgtcgcggccgggaagacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcgcactccgg
cggtggtgtgtccgagtccgagtacgcgcgctgggtcgacaccgtcgcgcagggcatcaagggcaacccgatcgtgatc
ctcgagcccgacgcgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttcctcaagtacgccgcca
agtcgctcaccctcaagggcgcgcgcgtctacatcgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaa
ccgcctcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcgaactaccagacgacggcggacagc
aaggcgtacggccagcagatctcgcagcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacggct
cgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgcccggtcgcggtgaacgacggctccggcctgga
cgcgctcctgtgggtcaagctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggccagtggtggcag
gagatcgccctggagatggcgcgcaacgccaggtggtaataatactagagaaagaggagaaatactagatgtccagatt
agataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactc
gcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcca
ttgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgc
taaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacag
tatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcg
ctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctac
tactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattc
ggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacg
ctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata