##gff-version 3
##sequence-region BBa_K561005 1 3290
BBa_K561005	.	CDS	163	2442	.	+	0	ID=BBa_K118028;Name=BBa_K118028
BBa_K561005	.	CDS	163	2439	.	+	0	ID=annotation1985223;Name=bglX coding sequence;Parent=BBa_K118028
BBa_K561005	.	engineered_region	2451	3290	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K561005	.	terminator	3162	3241	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K561005	.	stem_loop	3173	3216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K561005	.	engineered_region	3162	3241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K561005	.	CDS	2469	3128	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K561005	.	engineered_tag	3129	3153	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K561005	.	CDS	2472	3088	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K561005	.	tag	3089	3122	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K561005	.	ribosome_entry_site	2451	2462	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K561005	.	conserved_region	2455	2458	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K561005	.	terminator	3250	3290	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K561005	.	polyA_site	3277	3290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K561005	.	stop_codon	3283	3283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K561005	.	stem_loop	3257	3276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K561005	.	engineered_region	3250	3290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K561005	.	ribosome_entry_site	144	156	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K561005	.	ribosome_entry_site	144	156	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K561005	.	engineered_region	144	156	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K561005	.	ribosome_entry_site	150	153	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K561005	.	promoter	1	135	.	+	0	ID=BBa_K561001;Name=BBa_K561001
BBa_K561005	.	promoter	1	135	.	+	0	ID=annotation2146939;Name=vgb promoter;Parent=BBa_K561001
>BBa_K561005
acaggacgctggggttaaaagtatttgagttttgatgtggattaagttttaagaggcaataaagattataataagtgct
gctacaccatactgatgtatggcaaaaccataataatgaacttaaggaagaccctctactagagtcacacaggaaagta
ctagatgaaaaaaataaccgtattgatttccatctggctcagtgcagccgcttttttctcttgtgagaaaaaaacagaa
gcggggagtaaacctcaggcctttgataaagaagtacaggatctgttaaaaaatatgtcgctggaagaaaaagccggac
agatgacgcagatcgatatccgtaatttattgaacaacggatatggaaataccgatgaaaaattagataccgccagatt
aaaagaagccattcaaacctatcatgtcggttctatattaaattgcattcaggcatacacacctgaaaaatgggttgaa
ttgatttctcagatccagaacgaagcattgcaatcacctaataaaattccggttttatacggaacagatgccatgcatg
gtgttggctttattaaagatgccgtattgtttccgcataacataggtatggctgcatcccgcaacgatcaattggtttc
gcaggcagctcaggtaacgtcaacagaagcgcgttccgttggccttacctggaattttgctccggtgcttgacgtaggc
agagaaccttactggtcgcgctttgaagaaacctttggtgaagatgtatacataacaacacaaatgggttcagcagccg
tacaaatgatggaaggcagtgacctgacgtctaaaacaaacatagcttcctgcctgaaacactttattggatattcagc
acccaaaaatggtatagaccgcacacaatcacacattcctgaaattgtgctgcgcgaatattatctgcccccgttcaga
gaagcgatcaataaaggcgcttcatccattatgatcaactctgctgagatcaatggcattccgtgtcacggcaacaaat
ggctgttaacagatctgctccgtacagagcttggatttaccggaatggttgtatcggattgggaagatgtgatccgttt
gcacacctggcataaagtagctgctacgccgaaagaagccgtaatgatggccgttaacgcaggtgtggatatgagcatg
gtgccgaatgattactcgtttccgaaatatctggtagaattggtgaaagaaggaaaagtttctatggcgcgcatcgacg
aagcggtgggacgtattctgaccctgaaaatcaaactgggcttgatgaaaaatccattgccatccattgcagatgttgg
tgtagtgggttctgatgcacatcaacagatcgcactaaatgcagcgcgtgaatcaattacattattgaagaacgataaa
aatattctgcctcttgcaaaagataaaaaaatactattggttggtccggccgctaacagtttatctgcgctgcacagtt
catggtcatacacatggcagggaagcaatgaatcgctgtatcctgaaacaactaaaacaatccgtgaggcattagaagc
gagcggtaataaagctaacatcagaacgaatgcgactacaggatttgatgatgcagcaaattatgatgtttcctttatt
caaaaaaatacagcaggggtggatgtaattattgtgtgtgtaggtgaagctgcctatgccgaacagcctggagtaatca
aggatctcaatctgccggaagcgcagaagcaattgattgttgctgcaaagaaaacaggcaaacctgtcattgtatgcct
ggtagagggcaggccgcgtttgtttccggaagaagaagcacttgcagatgctgtaataatgtgctaccgtccgggcagc
aaaggtgcagacgcatttgcagagattctatacggtgacatcaatcccagcggtaagcttccgttcacctatccgagat
acgacggagatattactacatacgattataaatttaaggaaaccgagcagcagttaaaaccgggcgtgagtgaatttgt
tgcgttcaatccgcaatggccattcggacatggcttgagctacacaacgtttgcatattcaaaccttaacgtgaacaaa
agtaattttacgaagaacgatagtgtgctggtaacggtagacataagcaatacaggagcacgtacaggtaagatagccg
tagagttatattccagagaccacttcgcatcgattacgccttctgaaagacgcttacgtaaatacacaaagattgaact
gaaagcaggagagaagcaaaccgtttcgtttacgatcaaagcagcggatctgcaatttgtaaataaagatctgaaaaca
gtaaccgaagcaggcgcttttgatctgatgatcggaaatctccagacagaaatatattttaatgagtaataatactaga
gaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtc
ggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaa
ataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggga
aagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagta
catttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttttt
cactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatca
agtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgat
caccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaa
gtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata