BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K561001 1 BBa_K561001 vgb promoter, microaerobic 2011-09-28T11:00:00Z 2015-05-08T01:12:41Z vgb promoter comes from Vitreoscilla sp. Released HQ 2013 vgb promoter is induced under microaerobic conditions. false false _729_ 0 8637 9 In stock false We construct this promoter, according to the reference: "Construction of highly efficient E. coli expression systems containing low oxygen induced promoter and partition region" by Liu Tao et al. false HUA Yijie, MA Keyue, XIA Yu annotation2146939 1 vgb promoter range2146939 1 1 135 BBa_P0440 1 BBa_P0440 PoPS -> TetR [S0151] 2004-04-26T11:00:00Z 2015-05-08T01:14:10Z Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Randy Rettberg component944896 1 BBa_C0040 component944912 1 BBa_B0012 component944902 1 BBa_B0010 component944886 1 BBa_B0034 annotation944902 1 BBa_B0010 range944902 1 712 791 annotation944896 1 BBa_C0040 range944896 1 19 678 annotation944886 1 BBa_B0034 range944886 1 1 12 annotation944912 1 BBa_B0012 range944912 1 800 840 BBa_K561005 1 BBa_K561005 Pvgb+bglX+tetR 2011-10-02T11:00:00Z 2015-05-08T01:12:41Z vgb promoter from Vitreoscilla sp. Middle Layer of the Rainbo-film expression system for cellulose degradation. Beta-glucosidase gene (bglX) form genome sequence of Gram negative bacterium Cytophaga hutchinsonii (iGEM08_Edinburgh) is under the regulation of anaerobic promoter fdhF. The expression of tetR will repress Ptet, which in the device of upper layer expression in the biofilm. false false _729_ 0 6270 9 It's complicated false Beta-glucosidase is expressed in the middle layer of Rainbo-film expression system, catalyzing the conversion of cellobiose to D-glucose. false LI Ye, XIA Yu component2150315 1 BBa_K118028 component2150310 1 BBa_K561001 component2150328 1 BBa_P0440 component2150312 1 BBa_B0032 annotation2150315 1 BBa_K118028 range2150315 1 163 2442 annotation2150312 1 BBa_B0032 range2150312 1 144 156 annotation2150328 1 BBa_P0440 range2150328 1 2451 3290 annotation2150310 1 BBa_K561001 range2150310 1 1 135 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_C0040 1 tetR tetracycline repressor from transposon Tn10 (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999) Released HQ 2013 Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P> false true _1_ 0 24 7 In stock false References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman. annotation23329 1 tetR range23329 1 4 620 annotation23330 1 SsrA range23330 1 621 654 annotation2213989 1 Help:Barcodes range2213989 1 661 685 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K118028 1 BBa_K118028 beta-glucosidase gene bglX (chu_2268) from Cytophaga hutchinsonii 2008-10-22T11:00:00Z 2015-05-08T01:09:37Z Genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii. Released HQ 2013 This is a beta-glucosidase gene (bglX, locus chu_2268) from the genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii. false false _192_ 0 837 163 In stock true No special considerations. true Chris French annotation1985223 1 bglX coding sequence range1985223 1 1 2277 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K561001_sequence 1 acaggacgctggggttaaaagtatttgagttttgatgtggattaagttttaagaggcaataaagattataataagtgctgctacaccatactgatgtatggcaaaaccataataatgaacttaaggaagaccctc BBa_B0034_sequence 1 aaagaggagaaa BBa_B0032_sequence 1 tcacacaggaaag BBa_K561005_sequence 1 acaggacgctggggttaaaagtatttgagttttgatgtggattaagttttaagaggcaataaagattataataagtgctgctacaccatactgatgtatggcaaaaccataataatgaacttaaggaagaccctctactagagtcacacaggaaagtactagatgaaaaaaataaccgtattgatttccatctggctcagtgcagccgcttttttctcttgtgagaaaaaaacagaagcggggagtaaacctcaggcctttgataaagaagtacaggatctgttaaaaaatatgtcgctggaagaaaaagccggacagatgacgcagatcgatatccgtaatttattgaacaacggatatggaaataccgatgaaaaattagataccgccagattaaaagaagccattcaaacctatcatgtcggttctatattaaattgcattcaggcatacacacctgaaaaatgggttgaattgatttctcagatccagaacgaagcattgcaatcacctaataaaattccggttttatacggaacagatgccatgcatggtgttggctttattaaagatgccgtattgtttccgcataacataggtatggctgcatcccgcaacgatcaattggtttcgcaggcagctcaggtaacgtcaacagaagcgcgttccgttggccttacctggaattttgctccggtgcttgacgtaggcagagaaccttactggtcgcgctttgaagaaacctttggtgaagatgtatacataacaacacaaatgggttcagcagccgtacaaatgatggaaggcagtgacctgacgtctaaaacaaacatagcttcctgcctgaaacactttattggatattcagcacccaaaaatggtatagaccgcacacaatcacacattcctgaaattgtgctgcgcgaatattatctgcccccgttcagagaagcgatcaataaaggcgcttcatccattatgatcaactctgctgagatcaatggcattccgtgtcacggcaacaaatggctgttaacagatctgctccgtacagagcttggatttaccggaatggttgtatcggattgggaagatgtgatccgtttgcacacctggcataaagtagctgctacgccgaaagaagccgtaatgatggccgttaacgcaggtgtggatatgagcatggtgccgaatgattactcgtttccgaaatatctggtagaattggtgaaagaaggaaaagtttctatggcgcgcatcgacgaagcggtgggacgtattctgaccctgaaaatcaaactgggcttgatgaaaaatccattgccatccattgcagatgttggtgtagtgggttctgatgcacatcaacagatcgcactaaatgcagcgcgtgaatcaattacattattgaagaacgataaaaatattctgcctcttgcaaaagataaaaaaatactattggttggtccggccgctaacagtttatctgcgctgcacagttcatggtcatacacatggcagggaagcaatgaatcgctgtatcctgaaacaactaaaacaatccgtgaggcattagaagcgagcggtaataaagctaacatcagaacgaatgcgactacaggatttgatgatgcagcaaattatgatgtttcctttattcaaaaaaatacagcaggggtggatgtaattattgtgtgtgtaggtgaagctgcctatgccgaacagcctggagtaatcaaggatctcaatctgccggaagcgcagaagcaattgattgttgctgcaaagaaaacaggcaaacctgtcattgtatgcctggtagagggcaggccgcgtttgtttccggaagaagaagcacttgcagatgctgtaataatgtgctaccgtccgggcagcaaaggtgcagacgcatttgcagagattctatacggtgacatcaatcccagcggtaagcttccgttcacctatccgagatacgacggagatattactacatacgattataaatttaaggaaaccgagcagcagttaaaaccgggcgtgagtgaatttgttgcgttcaatccgcaatggccattcggacatggcttgagctacacaacgtttgcatattcaaaccttaacgtgaacaaaagtaattttacgaagaacgatagtgtgctggtaacggtagacataagcaatacaggagcacgtacaggtaagatagccgtagagttatattccagagaccacttcgcatcgattacgccttctgaaagacgcttacgtaaatacacaaagattgaactgaaagcaggagagaagcaaaccgtttcgtttacgatcaaagcagcggatctgcaatttgtaaataaagatctgaaaacagtaaccgaagcaggcgcttttgatctgatgatcggaaatctccagacagaaatatattttaatgagtaataatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0040_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_P0440_sequence 1 aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K118028_sequence 1 atgaaaaaaataaccgtattgatttccatctggctcagtgcagccgcttttttctcttgtgagaaaaaaacagaagcggggagtaaacctcaggcctttgataaagaagtacaggatctgttaaaaaatatgtcgctggaagaaaaagccggacagatgacgcagatcgatatccgtaatttattgaacaacggatatggaaataccgatgaaaaattagataccgccagattaaaagaagccattcaaacctatcatgtcggttctatattaaattgcattcaggcatacacacctgaaaaatgggttgaattgatttctcagatccagaacgaagcattgcaatcacctaataaaattccggttttatacggaacagatgccatgcatggtgttggctttattaaagatgccgtattgtttccgcataacataggtatggctgcatcccgcaacgatcaattggtttcgcaggcagctcaggtaacgtcaacagaagcgcgttccgttggccttacctggaattttgctccggtgcttgacgtaggcagagaaccttactggtcgcgctttgaagaaacctttggtgaagatgtatacataacaacacaaatgggttcagcagccgtacaaatgatggaaggcagtgacctgacgtctaaaacaaacatagcttcctgcctgaaacactttattggatattcagcacccaaaaatggtatagaccgcacacaatcacacattcctgaaattgtgctgcgcgaatattatctgcccccgttcagagaagcgatcaataaaggcgcttcatccattatgatcaactctgctgagatcaatggcattccgtgtcacggcaacaaatggctgttaacagatctgctccgtacagagcttggatttaccggaatggttgtatcggattgggaagatgtgatccgtttgcacacctggcataaagtagctgctacgccgaaagaagccgtaatgatggccgttaacgcaggtgtggatatgagcatggtgccgaatgattactcgtttccgaaatatctggtagaattggtgaaagaaggaaaagtttctatggcgcgcatcgacgaagcggtgggacgtattctgaccctgaaaatcaaactgggcttgatgaaaaatccattgccatccattgcagatgttggtgtagtgggttctgatgcacatcaacagatcgcactaaatgcagcgcgtgaatcaattacattattgaagaacgataaaaatattctgcctcttgcaaaagataaaaaaatactattggttggtccggccgctaacagtttatctgcgctgcacagttcatggtcatacacatggcagggaagcaatgaatcgctgtatcctgaaacaactaaaacaatccgtgaggcattagaagcgagcggtaataaagctaacatcagaacgaatgcgactacaggatttgatgatgcagcaaattatgatgtttcctttattcaaaaaaatacagcaggggtggatgtaattattgtgtgtgtaggtgaagctgcctatgccgaacagcctggagtaatcaaggatctcaatctgccggaagcgcagaagcaattgattgttgctgcaaagaaaacaggcaaacctgtcattgtatgcctggtagagggcaggccgcgtttgtttccggaagaagaagcacttgcagatgctgtaataatgtgctaccgtccgggcagcaaaggtgcagacgcatttgcagagattctatacggtgacatcaatcccagcggtaagcttccgttcacctatccgagatacgacggagatattactacatacgattataaatttaaggaaaccgagcagcagttaaaaccgggcgtgagtgaatttgttgcgttcaatccgcaatggccattcggacatggcttgagctacacaacgtttgcatattcaaaccttaacgtgaacaaaagtaattttacgaagaacgatagtgtgctggtaacggtagacataagcaatacaggagcacgtacaggtaagatagccgtagagttatattccagagaccacttcgcatcgattacgccttctgaaagacgcttacgtaaatacacaaagattgaactgaaagcaggagagaagcaaaccgtttcgtttacgatcaaagcagcggatctgcaatttgtaaataaagatctgaaaacagtaaccgaagcaggcgcttttgatctgatgatcggaaatctccagacagaaatatattttaatgagtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z