BBa_K562003 1 Salty_PduA Salty_PduAB BMC Components 2011-09-15T11:00:00Z 2015-05-08T01:12:41Z Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence. This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the PduA and PduB proteins from Salmonella enterica serovar Typhimurium LT2. These are components of the Salmonella bacterial microcompartment (BMC). The PduB gene product carries a C-terminal 6-His affinity/epitope tag. Production of the PduB protein has been verified by Western immunoblotting (anti-penta-His) and production of both PduA and PduB have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-AB in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 In stock false There were two native PstI sites within the pduB gene. These were removed using PCR-based site-directed mutagenesis. Care was taken not to alter the primary sequence of the gene product. The fragment also carries an engineered BamHI site at its extreme 3' end. false Frank Sargent annotation2129415 1 Hexa-Histidine Tag range2129415 1 1210 1227 annotation2129413 1 pduA gene range2129413 1 119 403 annotation2129412 1 synthetic RBS range2129412 1 107 112 annotation2129411 1 BBa_K562000 range2129411 1 1 103 annotation2129414 1 pduB gene range2129414 1 400 1209 BBa_K562003_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatgcaacaagaagcactaggaatggtagaaaccaaaggcttaaccgcagccatagaggccgctgatgcaatggttaagtcagccaatgtgatgttagtgggctatgaaaagattggctccgggctggtaaccgtcatcgtgcgcggcgatgttggcgcggtcaaagcggccaccgatgcaggtgccgcagccgcacgcaacgtgggtgaagtgaaagccgtacacgtcatcccacgccctcacaccgatgtagaaaaaatcttaccgaagggaattagccaatgagcagcaatgagctggtggaacagatcatggcgcaggtgattgcccgtgtggcaacgccggaacaacaggccatccctggtcaaccccaacctatacgagagacggctatggcagaaaaaagctgtagtttaacggaatttgtcgggaccgccattggcgataccctgggcctggttatcgccaacgtcgataccgccctgctggacgcgatgaaacttgagaagcgctatcgttccatcggcattctcggcgcccgcaccggcgcaggcccgcacattatggcggcggacgaagcggtgaaagccaccaacaccgaagtggtcagcattgagctgccgcgcgataccaaaggcggcgcgggccacggttcgctgattattttaggcggtaacgacgtttccgacgtcaagcgcggtatcgaagtcgcgctgaaagagctggaccgcaccttcggtgatgtttacggcaacgaagccggacatatcgagctgcaatacaccgctcgcgccagctatgcgctggaaaaagcgttcggcgcgccgattggccgcgcctgcggcattatcgtcggcgctccggcttccgtcggcgtactgatggccgataccgcgctgaagtcagccaacgttgaagtcgtggcgtacagctctccggcgcacggcaccagctttagtaacgaagctattctggtgatttccggcgactccggcgcggtccgtcaggcggtgacctccgcccgcgaaatcggcaaaaccgtccttgcgaccctcggttctgaaccgaaaaacgatcgtccgtcctacatccatcaccatcaccatcactaaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z