BBa_K562007 1 Salty_PduA Salty_PduABTU BMC Components 2011-09-15T11:00:00Z 2015-05-08T01:12:41Z Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence. This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the PduA and PduB proteins from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562003) AND proteins PduT and PduU. These are all components of the Salmonella bacterial microcompartment (BMC). The PduB and PduU gene product carry C-terminal 6-His affinity/epitope tags. Production of the PduB and PduU proteins has been verified by Western immunoblotting (anti-penta-His) and production of PduA, PduB, PduT and PduU have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-ABTU in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 It's complicated false Native PstI sites in pduB and pduU were removed by site-directed mutagenesis. The fragment also carries an engineered BamHI site at its extreme 3' end. false Frank Sargent annotation2129478 1 Hexa-Histidine Tag range2129478 1 2154 2171 annotation2129472 1 Hexa-Histidine Tag range2129472 1 1210 1227 annotation2129468 1 BBa_K562003 range2129468 1 1 1236 annotation2129469 1 synthetic RBS range2129469 1 107 112 annotation2129477 1 pduU gene range2129477 1 1806 2153 annotation2129470 1 pduA gene range2129470 1 119 403 annotation2129475 1 pduT gene range2129475 1 1252 1806 annotation2129474 1 synthetic RBS range2129474 1 1240 1245 annotation2129471 1 pduB gene range2129471 1 400 1209 BBa_K562007_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatgcaacaagaagcactaggaatggtagaaaccaaaggcttaaccgcagccatagaggccgctgatgcaatggttaagtcagccaatgtgatgttagtgggctatgaaaagattggctccgggctggtaaccgtcatcgtgcgcggcgatgttggcgcggtcaaagcggccaccgatgcaggtgccgcagccgcacgcaacgtgggtgaagtgaaagccgtacacgtcatcccacgccctcacaccgatgtagaaaaaatcttaccgaagggaattagccaatgagcagcaatgagctggtggaacagatcatggcgcaggtgattgcccgtgtggcaacgccggaacaacaggccatccctggtcaaccccaacctatacgagagacggctatggcagaaaaaagctgtagtttaacggaatttgtcgggaccgccattggcgataccctgggcctggttatcgccaacgtcgataccgccctgctggacgcgatgaaacttgagaagcgctatcgttccatcggcattctcggcgcccgcaccggcgcaggcccgcacattatggcggcggacgaagcggtgaaagccaccaacaccgaagtggtcagcattgagctgccgcgcgataccaaaggcggcgcgggccacggttcgctgattattttaggcggtaacgacgtttccgacgtcaagcgcggtatcgaagtcgcgctgaaagagctggaccgcaccttcggtgatgtttacggcaacgaagccggacatatcgagctgcaatacaccgctcgcgccagctatgcgctggaaaaagcgttcggcgcgccgattggccgcgcctgcggcattatcgtcggcgctccggcttccgtcggcgtactgatggccgataccgcgctgaagtcagccaacgttgaagtcgtggcgtacagctctccggcgcacggcaccagctttagtaacgaagctattctggtgatttccggcgactccggcgcggtccgtcaggcggtgacctccgcccgcgaaatcggcaaaaccgtccttgcgaccctcggttctgaaccgaaaaacgatcgtccgtcctacatccatcaccatcaccatcactaaggatcacacagaggaacaggtatgtctcaggctataggaattttagaactcaccagcatcgccaaaggcatggagcttggtgatgccatgctgaaaagcgccaacgtcgatctactggtcagcaagaccatctgtccggggaagtttttactgatgctgggcggcgatatcggcgctatccagcaggccattgaaaccggtacgtcgcaggccggcgagatgctcgtcgacagcctggtgctggcgaatattcatcccagcgtacttccggccatcagcggcctgaacagcgtcgataagcgccaggcggtaggtatcgtcgaaacctggagcgtggcggcctgcattagcgccgcagaccgggcggtgaaaggctcaaacgtcaccctggtgcgggtgcatatggccttcggtatcggcggtaaatgctacatggtggtggcgggcgacgtttctgacgtcaataacgccgtgacggttgccagcgaaagcgcgggcgagaaagggttgttggtttaccgttcggtgatcccacgcccgcatgaagccatgtggcgacagatggtggaggggtaatggaaagacaaccgacaacggatcgcatgattcaggaatacgtgccggggaaacaggtcactctcgcgcacctgattgctaatccagggaaagatctctttaagaagctgggcctgcaagatgcagtgtccgccattggcatccttaccatcacgccgagcgaagcatcgattatcgcctgcgatatcgccaccaaatccggcgcggtggaaattggttttctcgatcgctttaccggtgccgtggtgctgaccggcgacgtttctgccgtcgagtatgccctcaagcaggtaacgcgcacgctgggcgaaatgatgcagttcaccacttgctcgatcacccggacgcatcaccatcaccatcactaaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z