LOCUS       BBa_K562008             2495 bp    DNA     linear       26-Nov-2024
DEFINITION  Salty_PduABTUN BMC Components
ACCESSION   BBa_K562008
VERSION     BBa_K562008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..2180
                     /label=BBa_K562007
     RBS             107..112
                     /label=synthetic RBS
     CDS             119..403
                     /label=pduA gene
     CDS             400..1209
                     /label=pduB gene
     misc_feature    1210..1227
                     /label=Hexa-Histidine Tag
     RBS             1240..1245
                     /label=synthetic RBS
     CDS             1252..1806
                     /label=pduT gene
     CDS             1806..2153
                     /label=pduU gene
     misc_feature    2154..2171
                     /label=Hexa-Histidine Tag
     RBS             2184..2189
                     /label=synthetic RBS
     CDS             2196..2468
                     /label=pduN gene
     misc_feature    2469..2486
                     /label=Hexa-Histidine Tag
ORIGIN
        1 tgtcggttgg cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat
       61 aatgcggctt tgtttaatca tcatctacca cagaggagga tcacacagag gaacaggtat
      121 gcaacaagaa gcactaggaa tggtagaaac caaaggctta accgcagcca tagaggccgc
      181 tgatgcaatg gttaagtcag ccaatgtgat gttagtgggc tatgaaaaga ttggctccgg
      241 gctggtaacc gtcatcgtgc gcggcgatgt tggcgcggtc aaagcggcca ccgatgcagg
      301 tgccgcagcc gcacgcaacg tgggtgaagt gaaagccgta cacgtcatcc cacgccctca
      361 caccgatgta gaaaaaatct taccgaaggg aattagccaa tgagcagcaa tgagctggtg
      421 gaacagatca tggcgcaggt gattgcccgt gtggcaacgc cggaacaaca ggccatccct
      481 ggtcaacccc aacctatacg agagacggct atggcagaaa aaagctgtag tttaacggaa
      541 tttgtcggga ccgccattgg cgataccctg ggcctggtta tcgccaacgt cgataccgcc
      601 ctgctggacg cgatgaaact tgagaagcgc tatcgttcca tcggcattct cggcgcccgc
      661 accggcgcag gcccgcacat tatggcggcg gacgaagcgg tgaaagccac caacaccgaa
      721 gtggtcagca ttgagctgcc gcgcgatacc aaaggcggcg cgggccacgg ttcgctgatt
      781 attttaggcg gtaacgacgt ttccgacgtc aagcgcggta tcgaagtcgc gctgaaagag
      841 ctggaccgca ccttcggtga tgtttacggc aacgaagccg gacatatcga gctgcaatac
      901 accgctcgcg ccagctatgc gctggaaaaa gcgttcggcg cgccgattgg ccgcgcctgc
      961 ggcattatcg tcggcgctcc ggcttccgtc ggcgtactga tggccgatac cgcgctgaag
     1021 tcagccaacg ttgaagtcgt ggcgtacagc tctccggcgc acggcaccag ctttagtaac
     1081 gaagctattc tggtgatttc cggcgactcc ggcgcggtcc gtcaggcggt gacctccgcc
     1141 cgcgaaatcg gcaaaaccgt ccttgcgacc ctcggttctg aaccgaaaaa cgatcgtccg
     1201 tcctacatcc atcaccatca ccatcactaa ggatcacaca gaggaacagg tatgtctcag
     1261 gctataggaa ttttagaact caccagcatc gccaaaggca tggagcttgg tgatgccatg
     1321 ctgaaaagcg ccaacgtcga tctactggtc agcaagacca tctgtccggg gaagttttta
     1381 ctgatgctgg gcggcgatat cggcgctatc cagcaggcca ttgaaaccgg tacgtcgcag
     1441 gccggcgaga tgctcgtcga cagcctggtg ctggcgaata ttcatcccag cgtacttccg
     1501 gccatcagcg gcctgaacag cgtcgataag cgccaggcgg taggtatcgt cgaaacctgg
     1561 agcgtggcgg cctgcattag cgccgcagac cgggcggtga aaggctcaaa cgtcaccctg
     1621 gtgcgggtgc atatggcctt cggtatcggc ggtaaatgct acatggtggt ggcgggcgac
     1681 gtttctgacg tcaataacgc cgtgacggtt gccagcgaaa gcgcgggcga gaaagggttg
     1741 ttggtttacc gttcggtgat cccacgcccg catgaagcca tgtggcgaca gatggtggag
     1801 gggtaatgga aagacaaccg acaacggatc gcatgattca ggaatacgtg ccggggaaac
     1861 aggtcactct cgcgcacctg attgctaatc cagggaaaga tctctttaag aagctgggcc
     1921 tgcaagatgc agtgtccgcc attggcatcc ttaccatcac gccgagcgaa gcatcgatta
     1981 tcgcctgcga tatcgccacc aaatccggcg cggtggaaat tggttttctc gatcgcttta
     2041 ccggtgccgt ggtgctgacc ggcgacgttt ctgccgtcga gtatgccctc aagcaggtaa
     2101 cgcgcacgct gggcgaaatg atgcagttca ccacttgctc gatcacccgg acgcatcacc
     2161 atcaccatca ctaaggatca cacagaggaa caggtatgca tctggcacga gtcacgggcg
     2221 cggttgtctc cacgcaaaaa tcaccttctt tgattgggaa aaagctgctg ctggtgcgtc
     2281 gggtcagcgc cgatggcgaa ctccccgcct cgcccacctc cggcgatgaa gtggccgtgg
     2341 actccgtcgg cgcgggcgtc ggcgaactgg ttttgctcag cggcggctcc agcgccaggc
     2401 acgttttttc cgggccaaat gaggccattg acctcgccgt tgtcggcatt gtagatacgc
     2461 tttcgtgtca tcaccatcac catcactaag gatcc
//