##gff-version 3
##sequence-region BBa_K562008 1 2486
BBa_K562008	.	engineered_region	1	2180	.	+	0	ID=annotation2129495;Name=BBa_K562007
BBa_K562008	.	CDS	1806	2153	.	+	0	ID=annotation2129502;Name=pduU gene
BBa_K562008	.	CDS	400	1209	.	+	0	ID=annotation2129498;Name=pduB gene
BBa_K562008	.	ribosome_entry_site	107	112	.	+	0	ID=annotation2129496;Name=synthetic RBS
BBa_K562008	.	CDS	2196	2468	.	+	0	ID=annotation2129510;Name=pduN gene
BBa_K562008	.	CDS	119	403	.	+	0	ID=annotation2129497;Name=pduA gene
BBa_K562008	.	sequence_feature	1210	1227	.	+	0	ID=annotation2129499;Name=Hexa-Histidine Tag
BBa_K562008	.	sequence_feature	2469	2486	.	+	0	ID=annotation2129512;Name=Hexa-Histidine Tag
BBa_K562008	.	sequence_feature	2154	2171	.	+	0	ID=annotation2129503;Name=Hexa-Histidine Tag
BBa_K562008	.	ribosome_entry_site	1240	1245	.	+	0	ID=annotation2129500;Name=synthetic RBS
BBa_K562008	.	CDS	1252	1806	.	+	0	ID=annotation2129501;Name=pduT gene
BBa_K562008	.	ribosome_entry_site	2184	2189	.	+	0	ID=annotation2129507;Name=synthetic RBS
>BBa_K562008
tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatc
atcatctaccacagaggaggatcacacagaggaacaggtatgcaacaagaagcactaggaatggtagaaaccaaaggct
taaccgcagccatagaggccgctgatgcaatggttaagtcagccaatgtgatgttagtgggctatgaaaagattggctc
cgggctggtaaccgtcatcgtgcgcggcgatgttggcgcggtcaaagcggccaccgatgcaggtgccgcagccgcacgc
aacgtgggtgaagtgaaagccgtacacgtcatcccacgccctcacaccgatgtagaaaaaatcttaccgaagggaatta
gccaatgagcagcaatgagctggtggaacagatcatggcgcaggtgattgcccgtgtggcaacgccggaacaacaggcc
atccctggtcaaccccaacctatacgagagacggctatggcagaaaaaagctgtagtttaacggaatttgtcgggaccg
ccattggcgataccctgggcctggttatcgccaacgtcgataccgccctgctggacgcgatgaaacttgagaagcgcta
tcgttccatcggcattctcggcgcccgcaccggcgcaggcccgcacattatggcggcggacgaagcggtgaaagccacc
aacaccgaagtggtcagcattgagctgccgcgcgataccaaaggcggcgcgggccacggttcgctgattattttaggcg
gtaacgacgtttccgacgtcaagcgcggtatcgaagtcgcgctgaaagagctggaccgcaccttcggtgatgtttacgg
caacgaagccggacatatcgagctgcaatacaccgctcgcgccagctatgcgctggaaaaagcgttcggcgcgccgatt
ggccgcgcctgcggcattatcgtcggcgctccggcttccgtcggcgtactgatggccgataccgcgctgaagtcagcca
acgttgaagtcgtggcgtacagctctccggcgcacggcaccagctttagtaacgaagctattctggtgatttccggcga
ctccggcgcggtccgtcaggcggtgacctccgcccgcgaaatcggcaaaaccgtccttgcgaccctcggttctgaaccg
aaaaacgatcgtccgtcctacatccatcaccatcaccatcactaaggatcacacagaggaacaggtatgtctcaggcta
taggaattttagaactcaccagcatcgccaaaggcatggagcttggtgatgccatgctgaaaagcgccaacgtcgatct
actggtcagcaagaccatctgtccggggaagtttttactgatgctgggcggcgatatcggcgctatccagcaggccatt
gaaaccggtacgtcgcaggccggcgagatgctcgtcgacagcctggtgctggcgaatattcatcccagcgtacttccgg
ccatcagcggcctgaacagcgtcgataagcgccaggcggtaggtatcgtcgaaacctggagcgtggcggcctgcattag
cgccgcagaccgggcggtgaaaggctcaaacgtcaccctggtgcgggtgcatatggccttcggtatcggcggtaaatgc
tacatggtggtggcgggcgacgtttctgacgtcaataacgccgtgacggttgccagcgaaagcgcgggcgagaaagggt
tgttggtttaccgttcggtgatcccacgcccgcatgaagccatgtggcgacagatggtggaggggtaatggaaagacaa
ccgacaacggatcgcatgattcaggaatacgtgccggggaaacaggtcactctcgcgcacctgattgctaatccaggga
aagatctctttaagaagctgggcctgcaagatgcagtgtccgccattggcatccttaccatcacgccgagcgaagcatc
gattatcgcctgcgatatcgccaccaaatccggcgcggtggaaattggttttctcgatcgctttaccggtgccgtggtg
ctgaccggcgacgtttctgccgtcgagtatgccctcaagcaggtaacgcgcacgctgggcgaaatgatgcagttcacca
cttgctcgatcacccggacgcatcaccatcaccatcactaaggatcacacagaggaacaggtatgcatctggcacgagt
cacgggcgcggttgtctccacgcaaaaatcaccttctttgattgggaaaaagctgctgctggtgcgtcgggtcagcgcc
gatggcgaactccccgcctcgcccacctccggcgatgaagtggccgtggactccgtcggcgcgggcgtcggcgaactgg
ttttgctcagcggcggctccagcgccaggcacgttttttccgggccaaatgaggccattgacctcgccgttgtcggcat
tgtagatacgctttcgtgtcatcaccatcaccatcactaaggatcc