##gff-version 3
##sequence-region BBa_K562009 1 3287
BBa_K562009	.	sequence_feature	2469	2486	.	+	0	ID=annotation2129541;Name=Hexa-Histidine Tag
BBa_K562009	.	CDS	2511	2786	.	+	0	ID=annotation2129543;Name=pduJ gene
BBa_K562009	.	engineered_region	1	2495	.	+	0	ID=annotation2129530;Name=BBa_K562008
BBa_K562009	.	sequence_feature	2154	2171	.	+	0	ID=annotation2129538;Name=Hexa-Histidine Tag
BBa_K562009	.	ribosome_entry_site	2184	2189	.	+	0	ID=annotation2129539;Name=synthetic RBS
BBa_K562009	.	ribosome_entry_site	107	112	.	+	0	ID=annotation2129531;Name=synthetic RBS
BBa_K562009	.	CDS	119	403	.	+	0	ID=annotation2129532;Name=pduA gene
BBa_K562009	.	CDS	400	1209	.	+	0	ID=annotation2129533;Name=pduB gene
BBa_K562009	.	ribosome_entry_site	1240	1245	.	+	0	ID=annotation2129535;Name=synthetic RBS
BBa_K562009	.	ribosome_entry_site	2499	2504	.	+	0	ID=annotation2129542;Name=synthetic RBS
BBa_K562009	.	sequence_feature	3270	3287	.	+	0	ID=annotation2129545;Name=Hexa-Histidine Tag
BBa_K562009	.	CDS	1806	2153	.	+	0	ID=annotation2129537;Name=pduU gene
BBa_K562009	.	CDS	2811	3269	.	+	0	ID=annotation2129544;Name=pduK gene
BBa_K562009	.	sequence_feature	1210	1227	.	+	0	ID=annotation2129534;Name=Hexa-Histidine Tag
BBa_K562009	.	CDS	1252	1806	.	+	0	ID=annotation2129536;Name=pduT gene
BBa_K562009	.	CDS	2196	2468	.	+	0	ID=annotation2129540;Name=pduN gene
>BBa_K562009
tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatc
atcatctaccacagaggaggatcacacagaggaacaggtatgcaacaagaagcactaggaatggtagaaaccaaaggct
taaccgcagccatagaggccgctgatgcaatggttaagtcagccaatgtgatgttagtgggctatgaaaagattggctc
cgggctggtaaccgtcatcgtgcgcggcgatgttggcgcggtcaaagcggccaccgatgcaggtgccgcagccgcacgc
aacgtgggtgaagtgaaagccgtacacgtcatcccacgccctcacaccgatgtagaaaaaatcttaccgaagggaatta
gccaatgagcagcaatgagctggtggaacagatcatggcgcaggtgattgcccgtgtggcaacgccggaacaacaggcc
atccctggtcaaccccaacctatacgagagacggctatggcagaaaaaagctgtagtttaacggaatttgtcgggaccg
ccattggcgataccctgggcctggttatcgccaacgtcgataccgccctgctggacgcgatgaaacttgagaagcgcta
tcgttccatcggcattctcggcgcccgcaccggcgcaggcccgcacattatggcggcggacgaagcggtgaaagccacc
aacaccgaagtggtcagcattgagctgccgcgcgataccaaaggcggcgcgggccacggttcgctgattattttaggcg
gtaacgacgtttccgacgtcaagcgcggtatcgaagtcgcgctgaaagagctggaccgcaccttcggtgatgtttacgg
caacgaagccggacatatcgagctgcaatacaccgctcgcgccagctatgcgctggaaaaagcgttcggcgcgccgatt
ggccgcgcctgcggcattatcgtcggcgctccggcttccgtcggcgtactgatggccgataccgcgctgaagtcagcca
acgttgaagtcgtggcgtacagctctccggcgcacggcaccagctttagtaacgaagctattctggtgatttccggcga
ctccggcgcggtccgtcaggcggtgacctccgcccgcgaaatcggcaaaaccgtccttgcgaccctcggttctgaaccg
aaaaacgatcgtccgtcctacatccatcaccatcaccatcactaaggatcacacagaggaacaggtatgtctcaggcta
taggaattttagaactcaccagcatcgccaaaggcatggagcttggtgatgccatgctgaaaagcgccaacgtcgatct
actggtcagcaagaccatctgtccggggaagtttttactgatgctgggcggcgatatcggcgctatccagcaggccatt
gaaaccggtacgtcgcaggccggcgagatgctcgtcgacagcctggtgctggcgaatattcatcccagcgtacttccgg
ccatcagcggcctgaacagcgtcgataagcgccaggcggtaggtatcgtcgaaacctggagcgtggcggcctgcattag
cgccgcagaccgggcggtgaaaggctcaaacgtcaccctggtgcgggtgcatatggccttcggtatcggcggtaaatgc
tacatggtggtggcgggcgacgtttctgacgtcaataacgccgtgacggttgccagcgaaagcgcgggcgagaaagggt
tgttggtttaccgttcggtgatcccacgcccgcatgaagccatgtggcgacagatggtggaggggtaatggaaagacaa
ccgacaacggatcgcatgattcaggaatacgtgccggggaaacaggtcactctcgcgcacctgattgctaatccaggga
aagatctctttaagaagctgggcctgcaagatgcagtgtccgccattggcatccttaccatcacgccgagcgaagcatc
gattatcgcctgcgatatcgccaccaaatccggcgcggtggaaattggttttctcgatcgctttaccggtgccgtggtg
ctgaccggcgacgtttctgccgtcgagtatgccctcaagcaggtaacgcgcacgctgggcgaaatgatgcagttcacca
cttgctcgatcacccggacgcatcaccatcaccatcactaaggatcacacagaggaacaggtatgcatctggcacgagt
cacgggcgcggttgtctccacgcaaaaatcaccttctttgattgggaaaaagctgctgctggtgcgtcgggtcagcgcc
gatggcgaactccccgcctcgcccacctccggcgatgaagtggccgtggactccgtcggcgcgggcgtcggcgaactgg
ttttgctcagcggcggctccagcgccaggcacgttttttccgggccaaatgaggccattgacctcgccgttgtcggcat
tgtagatacgctttcgtgtcatcaccatcaccatcactaaggatcacacagaggaacaggtatgaataacgcactggga
ctggttgaaacaaaagggctggtcggcgccattgaagccgccgatgcaatggttaaatccgccaacgtacagctggtgg
gctacgaaaaaattggttctggcctggtgaccgtcatggttcgcggcgatgtcggcgcggttaaagcagccgtagatgc
cggcagcgcagcggcaagcgtcgtgggtgaagtgaaatcctgccacgttatcccgcgtccgcacagcgatgttgaggcc
attttaccgaaatcagcctaatcgatggcgaataaggagcaccgcgtgaagcaatcactgggattacttgaagtttgtg
gtctggcactggctatcagctgcgccgatatcatggcgaaatccgcttctatcacgctgctcgccctcgaaaagaccaa
tggttcaggctggatggtgattaaaattaccggtgatgtggcctccgttcaggcggctatcaccaccggtgcgcatttc
gccgaacagtggaatggcctggtggcccacaaggttatcgccaggcccggagaagggatcctgctcgcagagacaccct
ccccctccgtcattgaacctgagcctgaagcgtcagagatagctgatgtcgtttctgaagcgccagccgaagaggcccc
gcaggaatcagaactggtcagctgcaatctgtgtctggatccaaaatgtccgcgccagaagggcgaacctcgtacgctt
tgcattcattccggcaagcgaggtgaagcgcatcaccatcaccatcactaaggatcc