BBa_K562009 1 Salty_PduA Salty_PduABTUNJK BMC Components 2011-09-15T11:00:00Z 2015-05-08T01:12:41Z Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence. This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the PduA, PduB, PduT, PduU and PduN proteins from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562008) AND proteins PduJ and PduK. These are all components of the Salmonella bacterial microcompartment (BMC). The PduB, PduU, PduN and PduK gene products all carry C-terminal 6-His affinity/epitope tags. Production of the PduB, PduU, PduN and PduK proteins has been verified by Western immunoblotting (anti-penta-His) and production of PduA, PduB, PduT, PduU, PduN, PduJ and PduK has been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-ABTUNJK in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 It's complicated false Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3' end, but please note that pduK contains two native BamHI sites that have not been removed. false Frank Sargent annotation2129541 1 Hexa-Histidine Tag range2129541 1 2469 2486 annotation2129543 1 pduJ gene range2129543 1 2511 2786 annotation2129530 1 BBa_K562008 range2129530 1 1 2495 annotation2129538 1 Hexa-Histidine Tag range2129538 1 2154 2171 annotation2129539 1 synthetic RBS range2129539 1 2184 2189 annotation2129531 1 synthetic RBS range2129531 1 107 112 annotation2129532 1 pduA gene range2129532 1 119 403 annotation2129533 1 pduB gene range2129533 1 400 1209 annotation2129535 1 synthetic RBS range2129535 1 1240 1245 annotation2129542 1 synthetic RBS range2129542 1 2499 2504 annotation2129545 1 Hexa-Histidine Tag range2129545 1 3270 3287 annotation2129537 1 pduU gene range2129537 1 1806 2153 annotation2129544 1 pduK gene range2129544 1 2811 3269 annotation2129534 1 Hexa-Histidine Tag range2129534 1 1210 1227 annotation2129536 1 pduT gene range2129536 1 1252 1806 annotation2129540 1 pduN gene range2129540 1 2196 2468 BBa_K562009_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatgcaacaagaagcactaggaatggtagaaaccaaaggcttaaccgcagccatagaggccgctgatgcaatggttaagtcagccaatgtgatgttagtgggctatgaaaagattggctccgggctggtaaccgtcatcgtgcgcggcgatgttggcgcggtcaaagcggccaccgatgcaggtgccgcagccgcacgcaacgtgggtgaagtgaaagccgtacacgtcatcccacgccctcacaccgatgtagaaaaaatcttaccgaagggaattagccaatgagcagcaatgagctggtggaacagatcatggcgcaggtgattgcccgtgtggcaacgccggaacaacaggccatccctggtcaaccccaacctatacgagagacggctatggcagaaaaaagctgtagtttaacggaatttgtcgggaccgccattggcgataccctgggcctggttatcgccaacgtcgataccgccctgctggacgcgatgaaacttgagaagcgctatcgttccatcggcattctcggcgcccgcaccggcgcaggcccgcacattatggcggcggacgaagcggtgaaagccaccaacaccgaagtggtcagcattgagctgccgcgcgataccaaaggcggcgcgggccacggttcgctgattattttaggcggtaacgacgtttccgacgtcaagcgcggtatcgaagtcgcgctgaaagagctggaccgcaccttcggtgatgtttacggcaacgaagccggacatatcgagctgcaatacaccgctcgcgccagctatgcgctggaaaaagcgttcggcgcgccgattggccgcgcctgcggcattatcgtcggcgctccggcttccgtcggcgtactgatggccgataccgcgctgaagtcagccaacgttgaagtcgtggcgtacagctctccggcgcacggcaccagctttagtaacgaagctattctggtgatttccggcgactccggcgcggtccgtcaggcggtgacctccgcccgcgaaatcggcaaaaccgtccttgcgaccctcggttctgaaccgaaaaacgatcgtccgtcctacatccatcaccatcaccatcactaaggatcacacagaggaacaggtatgtctcaggctataggaattttagaactcaccagcatcgccaaaggcatggagcttggtgatgccatgctgaaaagcgccaacgtcgatctactggtcagcaagaccatctgtccggggaagtttttactgatgctgggcggcgatatcggcgctatccagcaggccattgaaaccggtacgtcgcaggccggcgagatgctcgtcgacagcctggtgctggcgaatattcatcccagcgtacttccggccatcagcggcctgaacagcgtcgataagcgccaggcggtaggtatcgtcgaaacctggagcgtggcggcctgcattagcgccgcagaccgggcggtgaaaggctcaaacgtcaccctggtgcgggtgcatatggccttcggtatcggcggtaaatgctacatggtggtggcgggcgacgtttctgacgtcaataacgccgtgacggttgccagcgaaagcgcgggcgagaaagggttgttggtttaccgttcggtgatcccacgcccgcatgaagccatgtggcgacagatggtggaggggtaatggaaagacaaccgacaacggatcgcatgattcaggaatacgtgccggggaaacaggtcactctcgcgcacctgattgctaatccagggaaagatctctttaagaagctgggcctgcaagatgcagtgtccgccattggcatccttaccatcacgccgagcgaagcatcgattatcgcctgcgatatcgccaccaaatccggcgcggtggaaattggttttctcgatcgctttaccggtgccgtggtgctgaccggcgacgtttctgccgtcgagtatgccctcaagcaggtaacgcgcacgctgggcgaaatgatgcagttcaccacttgctcgatcacccggacgcatcaccatcaccatcactaaggatcacacagaggaacaggtatgcatctggcacgagtcacgggcgcggttgtctccacgcaaaaatcaccttctttgattgggaaaaagctgctgctggtgcgtcgggtcagcgccgatggcgaactccccgcctcgcccacctccggcgatgaagtggccgtggactccgtcggcgcgggcgtcggcgaactggttttgctcagcggcggctccagcgccaggcacgttttttccgggccaaatgaggccattgacctcgccgttgtcggcattgtagatacgctttcgtgtcatcaccatcaccatcactaaggatcacacagaggaacaggtatgaataacgcactgggactggttgaaacaaaagggctggtcggcgccattgaagccgccgatgcaatggttaaatccgccaacgtacagctggtgggctacgaaaaaattggttctggcctggtgaccgtcatggttcgcggcgatgtcggcgcggttaaagcagccgtagatgccggcagcgcagcggcaagcgtcgtgggtgaagtgaaatcctgccacgttatcccgcgtccgcacagcgatgttgaggccattttaccgaaatcagcctaatcgatggcgaataaggagcaccgcgtgaagcaatcactgggattacttgaagtttgtggtctggcactggctatcagctgcgccgatatcatggcgaaatccgcttctatcacgctgctcgccctcgaaaagaccaatggttcaggctggatggtgattaaaattaccggtgatgtggcctccgttcaggcggctatcaccaccggtgcgcatttcgccgaacagtggaatggcctggtggcccacaaggttatcgccaggcccggagaagggatcctgctcgcagagacaccctccccctccgtcattgaacctgagcctgaagcgtcagagatagctgatgtcgtttctgaagcgccagccgaagaggccccgcaggaatcagaactggtcagctgcaatctgtgtctggatccaaaatgtccgcgccagaagggcgaacctcgtacgctttgcattcattccggcaagcgaggtgaagcgcatcaccatcaccatcactaaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z