BBa_K562010
1
PduD20-mCh
Salty_PduD20-mCherry
2011-09-16T11:00:00Z
2015-05-08T01:12:41Z
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and an unknown cloning vector encoding mCherry.
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 20 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562001), which is itself fused in-frame to mCherry. The mCherry gene product also carries a C-terminal HA epitope tag. Production of PduD20-mCherry has been verified by Western immunoblotting (anti-mCherry). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D20-mCh in the Sargent Laboratory, Dundee, UK.
false
false
_730_
0
8083
9
It's complicated
false
The single natural pstI site within the mCherry gene has been removed.
false
Frank Sargent
annotation2130312
1
HA epitope tag
range2130312
1
890
916
annotation2130308
1
BBa_K562001
range2130308
1
1
184
annotation2130309
1
synthetic RBS
range2130309
1
107
112
annotation2130311
1
mCherry
range2130311
1
185
889
annotation2130310
1
PduD20 Tag
range2130310
1
119
178
BBa_K562010_sequence
1
tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatggaaattaatgaaaaattgctgcgccagataattgaagacgtactccgcgatatgaagtctagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctacaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtatccgtacgatgtgccggactatgcgtaaggatcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z