BBa_K562011 1 PduD40-mCh Salty_PduD40-mCherry 2011-09-16T11:00:00Z 2015-05-08T01:12:41Z Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and an unknown cloning vector. This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to mCherry. The mCherry gene product also carries a C-terminal HA epitope tag. Production of PduD40-mCherry has been verified by Western immunoblotting (anti-mCherry). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-mCh in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 It's complicated false The natural pstI site within mCherry has been removed. false Frank Sargent annotation2130317 1 BBa_K562002 range2130317 1 1 244 annotation2130333 1 HA epitope tag range2130333 1 950 976 annotation2130320 1 mCherry range2130320 1 245 949 annotation2130318 1 synthetic RBS range2130318 1 107 112 annotation2130319 1 PduD40 Tag range2130319 1 119 238 BBa_K562011_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatggaaattaatgaaaaattgctgcgccagataattgaagacgtactccgcgatatgaagggcagcgataaacccgtctcgtttaatgcgcctgcggcatccacagcaccacagaccgcttctagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctacaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtatccgtacgatgtgccggactatgcgtaaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z