BBa_K562012 1 PduD20-GFP Salty_PduD20-GFP 2011-09-16T11:00:00Z 2015-05-08T01:12:41Z Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and an unknown cloning vector encoding GFP. This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 20 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562001), which is itself fused in-frame to GFP. The GFP gene product also carries a C-terminal HA epitope tag. Production of PduD20-GFP has been verified by Western immunoblotting (anti-GFP). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D20-GFP in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 It's complicated false None false Frank Sargent annotation2130995 1 HA epitope tag range2130995 1 896 922 annotation2130992 1 synthetic RBS range2130992 1 107 112 annotation2130993 1 PduD20 Tag range2130993 1 119 178 annotation2130994 1 GFP range2130994 1 185 895 annotation2130991 1 BBa_K562001 range2130991 1 1 184 BBa_K562012_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatggaaattaatgaaaaattgctgcgccagataattgaagacgtactccgcgatatgaagtctagaagtaaaggagaagaacttttcactggagtggtcccagttcttgttgaattagatggcgatgttaatgggcaaaaattctctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaattttatttgcactactgggaagctacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttctcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattttacaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaaatggaatacaactataactcacataatgtatacatcatgggagacaaaccaaagaatggcatcaaagttaacttcaaaattagacacaacattaaagatggaagcgttcaattagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttccaaagatcccaacgaaaagagagatcacatgatccttcttgagtttgtaacagctgctaggattacacatggcatggatgaactatacaaatatccgtacgatgtgccggactatgcgtaaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z